Patents by Inventor John C. Owicki
John C. Owicki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9683994Abstract: The present invention provides novel methods for performing pulsed field mobility shift assays in microfluidic devices. In particular, the methods of the invention utilize differences between electrophoretic mobilities (e.g., as between reactants and products, especially in non-fluorogenic reactions) in order to separate the species and thus analyze the reaction.Type: GrantFiled: July 9, 2012Date of Patent: June 20, 2017Assignee: Caliper Life Sciences, Inc.Inventors: Andrea W. Chow, John C. Owicki, J. Wallace Parce
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Publication number: 20120273353Abstract: The present invention provides novel methods for performing pulsed field mobility shift assays in microfluidic devices. In particular, the methods of the invention utilize differences between electrophoretic mobilities (e.g., as between reactants and products, especially in non-fluorogenic reactions) in order to separate the species and thus analyze the reaction.Type: ApplicationFiled: July 9, 2012Publication date: November 1, 2012Applicant: Caliper Life Sciences, Inc.Inventors: Andrea W. Chow, John C. Owicki, J. Wallace Parce
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Patent number: 8241883Abstract: The present invention provides novel microfluidic devices and methods for performing pulsed field mobility shift assays in microfluidic devices. In particular the devices and methods of the invention utilize differences between electrophoretic mobilities (e.g., as between reactants and products, especially in non-fluorogenic reactions) in order to separate the species and thus analyze the reaction.Type: GrantFiled: April 25, 2006Date of Patent: August 14, 2012Assignee: Caliper Life Sciences, Inc.Inventors: Andrea W. Chow, John C. Owicki, J. Wallace Parce
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Patent number: 7888292Abstract: Methods of detecting a component of interest, a change in charge, a pH, a cellular response using nanosensors are provided. Nanosensors, including nanowires and nanowire arrays comprising functionalized and/or non-functionalized nanowires are provided. Nanosensors are used for detection in cellular fragmentation, multiple concentration analysis, glucose detection, and intracellular analysis.Type: GrantFiled: October 18, 2007Date of Patent: February 15, 2011Assignee: Nanosys, Inc.Inventors: Larry Bock, R. Hugh Daniels, Stephen Empedocles, John C. Owicki
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Patent number: 6982431Abstract: Systems, and components thereof, for analyzing samples. These systems include apparatus and methods for generating, transmitting, detecting, and/or analyzing light, including without limitation high-throughput optical screening devices for analyzing samples at one or more assay sites. These systems also include apparatus and methods for supporting samples for analysis, including without limitation multiwell sample holders such as microplates.Type: GrantFiled: August 13, 2002Date of Patent: January 3, 2006Assignee: Molecular Devices CorporationInventors: Douglas N. Modlin, Glenn R. Edwards, John C. Owicki, Michael T. Taylor, Samuel A. Marquiss
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Patent number: 6825921Abstract: Apparatus and methods for combining multiple modes and methods of optical detection, postprocessing, and/or feedback loops in bioanalytical measurements. The methods may include (1) positioning a composition at an examination site in a multi-mode instrument; (2) detecting light transmitted form the composition using the multi-mode instrument in a first optical measurement mode; (3) detecting light transmitted from the composition using the multi-mode instrument in a second optical measurement mode, the second mode being different than the first mode; and (4) computing a quantity related to a property of the composition using the light detected in at least one of the optical measurement modes. These steps may be performed on one or more samples, simultaneously and/or sequentially. The quantity may relate to photoluminescence, chemiluminescence, absorption, and/or scattering, among others.Type: GrantFiled: November 10, 2000Date of Patent: November 30, 2004Assignee: Molecular Devices CorporationInventors: Douglas N. Modlin, John C. Owicki, Jon F. Petersen, Todd E. French, Carl L. Wright, Jeanne A. Ruiz, Lorne E. Bechtel
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Publication number: 20030127609Abstract: Systems, and components thereof, for analyzing samples. These systems include apparatus and methods for generating, transmitting, detecting, and/or analyzing light, including without limitation high-throughput optical screening devices for analyzing samples at one or more assay sites. These systems also include apparatus and methods for supporting samples for analysis, including without limitation multiwell sample holders such as microplates.Type: ApplicationFiled: August 13, 2002Publication date: July 10, 2003Inventors: Amer El-Hage, Christopher G. Cesar, Douglas N. Modlin, Glenn R. Edwards, John C. Owicki, Joh W. Meadows, Michael T. Taylor, Samuel A. Marquiss, Jonathan F. Petersen, David P. Stumbo
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Patent number: 6503719Abstract: Methods and apparatus for detecting polynucleotide hybridization in luminescence-based assays. The methods may include (1) contacting a sample polynucleotide with a reference polynucleotide at an assay site, where at least one of the polynucleotides is capable of emitting luminescence, (2) illuminating the assay site with light capable of stimulating such luminescence, (3) detecting light transmitted from the assay site, and (4) deriving information relating to the extent of hybridization between the sample and reference polynucleotides based on the detected light. The methods may further include (1) illuminating with and/or detecting polarized light, (2) deriving information relating to the sequence of the sample polynucleotide from the extent of hybridization, and (3) converting the light to a signal and distinguishing between a portion of the signal attributable to luminescence and a portion attributable to background.Type: GrantFiled: January 22, 2001Date of Patent: January 7, 2003Assignee: LJL BioSystems, Inc.Inventors: Douglas N. Modlin, Todd E. French, Jon F. Petersen, John C. Owicki
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Patent number: 6488892Abstract: Sample-holding devices and systems for efficiently utilizing relatively small-volume fluid samples.Type: GrantFiled: January 5, 2000Date of Patent: December 3, 2002Assignee: LJL BioSystems, Inc.Inventors: William G. Burton, Douglas N Modlin, Derrick A. Richardson, Jon F. Petersen, Joseph S. Leytes, John C. Owicki, Amer El-Hage, Lev J. Leytes
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Patent number: 6486947Abstract: Devices and methods for containing and analyzing small sample volumes that are sandwiched between solid surfaces. The devices may include an automated drive mechanism that controls the relative positions of the surfaces and an environmental-control mechanism that controls the humidity, temperature, and/or other environmental conditions around the small sample volume. In some embodiments, at least one of the surfaces has a light-transmissive window for allowing optical analysis of a sample contained between the surfaces.Type: GrantFiled: January 22, 2001Date of Patent: November 26, 2002Assignee: LJL BioSystems, Inc.Inventors: Douglas N. Modlin, Amer El-Hage, John C. Owicki
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Patent number: 6483582Abstract: Apparatus and methods for measuring light transmitted from a sample. The apparatus may include a stage, a light source, and a detector. The stage may be configured to hold a microplate having a plurality of sample wells. The apparatus may be configured to take frequency-domain time-resolved measurements of one or more of luminescence lifetimes and reorientational correlation times of a luminescent analyte in the sample.Type: GrantFiled: January 19, 2001Date of Patent: November 19, 2002Assignee: LJL BioSystems, Inc.Inventors: Douglas N. Modlin, Todd E. French, John C. Owicki
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Publication number: 20020158212Abstract: Frequency-domain light detection systems and components and uses thereof for performing time-resolved luminescence assays. The systems may include methods for identifying and/or correcting for background and/or quenching, among others. The systems also may include apparatus for increasing duty cycle and/or sensitivity, among others.Type: ApplicationFiled: November 12, 2001Publication date: October 31, 2002Inventors: Todd E. French, John C. Owicki, Douglas N. Modlin
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Patent number: 6466316Abstract: Apparatus and methods for measuring light transmitted from a sample. The apparatus may include a stage, a light source, and a detector. The stage may be configured to hold a microplate having a plurality of sample wells. The apparatus may be configured to take measurements of one or more of absorbance, scattering, reflectance and luminescence. The apparatus may permit simultaneous measurements of two or more of these properties.Type: GrantFiled: January 19, 2001Date of Patent: October 15, 2002Assignee: LJL BioSystems, Inc.Inventors: Douglas N. Modlin, Jon F. Petersen, John C Owicki
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Publication number: 20010046673Abstract: Methods and apparatus for detecting polynucleotide hybridization in luminescence-based assays. The methods may include (1) allele-specific hybridization, using luminescence detection, (2) allele-specific oligonucleotide ligation, using dye-labeled oligonucleotide ligation with luminescence resonance energy transfer (FRET) detection, and (3) allele-specific nucleotide incorporation, using primer extension with luminescence polarization (FP) detection. More specifically, the methods may include (1) locating a sample containing a nucleic acid material at an examination site, (2) illuminating the sample, (3) detecting light transmitted from the sample, and (4) determining the presence, absence, and/or identity of a nucleic acid target in the sample using the light transmitted from the sample.Type: ApplicationFiled: January 25, 2001Publication date: November 29, 2001Applicant: LJL BioSystems, Inc.Inventors: Todd E. French, Douglas N. Modlin, John C. Owicki, James S. Richey, Lev J. Leytes, Enal S. Razvi, Yan Zhang-Klompus, Peter R. Panfili
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Publication number: 20010034025Abstract: Methods and apparatus for detecting polynucleotide hybridization in luminescence-based assays. The methods may include (1) contacting a sample polynucleotide with a reference polynucleotide at an assay site, where at least one of the polynucleotides is capable of emitting luminescence, (2) illuminating the assay site with light capable of stimulating such luminescence, (3) detecting light transmitted from the assay site, and (4) deriving information relating to the extent of hybridization between the sample and reference polynucleotides based on the detected light. The methods may further include (1) illuminating with and/or detecting polarized light, (2) deriving information relating to the sequence of the sample polynucleotide from the extent of hybridization, and (3) converting the light to a signal and distinguishing between a portion of the signal attributable to luminescence and a portion attributable to background.Type: ApplicationFiled: January 22, 2001Publication date: October 25, 2001Applicant: LJL BioSystems, Inc.Inventors: Douglas N. Modlin, Todd E. French, Jon F. Petersen, John C. Owicki
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Patent number: 6297018Abstract: Methods and apparatus for detecting polynucleotide hybridization in luminescence-based assays. The methods may include (1) allele-specific hybridization, using luminescence detection, (2) allele-specific oligonucleotide ligation, using dye-labeled oligonucleotide ligation with luminescence resonance energy transfer (FRET) detection, and (3) allele-specific nucleotide incorporation, using primer extension with luminescence polarization (FP) detection. More specifically, the methods may include (1) locating a sample containing a nucleic acid material at an examination site, (2) illuminating the sample, (3) detecting light transmitted from the sample, and (4) determining the presence, absence, and/or identity of a nucleic acid target in the sample using the light transmitted from the sample.Type: GrantFiled: January 28, 2000Date of Patent: October 2, 2001Assignee: LJL BioSystems, Inc.Inventors: Todd E. French, Douglas N. Modlin, John C. Owicki, James S. Richey, Lev J. Leytes, Enal S. Razvi
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Publication number: 20010021514Abstract: Luminescent metal-ligand complexes and/or complementary energy transfer acceptors for use in luminescence assays. The complexes and/or acceptors may be used in free, reactive, and/or conjugated form, alone or mixed with other compounds. Preferred luminescence assays include luminescence polarization and luminescence resonance energy transfer assays, among others.Type: ApplicationFiled: January 22, 2001Publication date: September 13, 2001Applicant: LJL BioSystems, Inc.Inventors: Ewald A. Terpetschnig, Dan-Hui Yang, John C. Owicki, Yan Zhang
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Publication number: 20010018194Abstract: Apparatus, methods, compositions, and kits for improved luminescence assays. These improvements include, among others, the use of mass labeling in luminescence polarization assays, diffusion enhancements in luminescence resonance energy transfer assays, and labeled and/or unlabeled particulates in various luminescence assays.Type: ApplicationFiled: January 23, 2001Publication date: August 30, 2001Applicant: LJL BioSystems, Inc.Inventors: Ewald A. Terpetschnig, John C. Owicki, Sudhir S. Deshpande
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Publication number: 20010016330Abstract: Apparatus and methods for identifying and correcting for quenching in luminescence assays using luminescence lifetimes and/or luminescence intensities. One aspect of the invention involves identifying quenching using combinations of luminescence lifetimes and/or intensities. Another aspect of the invention involves correcting for quenching by eliminating false positives or false negatives due to quenching in luminescence assays.Type: ApplicationFiled: January 19, 2001Publication date: August 23, 2001Applicant: LJL BioSystems, Inc.Inventors: John C. Owicki, Douglas N. Modlin, Todd E. French
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Publication number: 20010007496Abstract: Apparatus and methods for measuring light transmitted from a sample. The apparatus may include a stage, a light source, and a detector. The stage may be configured to hold a microplate having a plurality of sample wells. The apparatus may be configured to take measurements of one or more of absorbance, scattering, reflectance and luminescence. The apparatus may permit simultaneous measurements of two or more of these properties.Type: ApplicationFiled: January 19, 2001Publication date: July 12, 2001Applicant: LJL BioSystems, Inc.Inventors: Douglas N. Modlin, Jon F. Petersen, John C. Owicki