Abstract: The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.

Abstract: The present invention relates to a nanofilter formed by using a porous ultrafiltration membrane as a precursor, and carefully controlling reaction conditions so as to maintain sufficient hydrophilic nature of the membrane while causing the pore structure to close to a nanofilter range (less than 400 Daltons). This produces a solvent stable cellulose nanofiltration membrane capable of operating at satisfactory flux in aqueous solutions, and being low binding to organic biomaterials.

Abstract: The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.

Abstract: The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.

Abstract: The present invention relates to a nanofilter formed by using a porous ultrafiltration membrane as a precursor, and carefully controlling reaction conditions so as to maintain sufficient hydrophilic nature of the membrane while causing the pore structure to close to a nanofilter range (less than 400 Daltons). This produces a solvent stable cellulose nanofiltration membrane capable of operating at satisfactory flux in aqueous solutions, and being low binding to organic biomaterials.

Abstract: The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.

Abstract: The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.

Abstract: The present invention provides porous media or membranes having a surface coating that includes a cross-linked terpolymer which has a superior combination of properties, including heat stable biomolecule resistant adsorptive properties, resistance to strong alkaline solutions, and low levels of extractable matter. In some preferred embodiments, the porous media is a porous membrane.

Abstract: Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.

Abstract: Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.

Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

Abstract: The present invention provides porous media or membranes having a surface coating that includes a cross-linked terpolymer which has a superior combination of properties, including heat stable biomolecule resistant adsorptive properties, resistance to strong alkaline solutions, and low levels of extractable matter. In some preferred embodiments, the porous media is a porous membrane.

Abstract: The present invention provides novel and improved stimulus responsive polymers and methods of using the same for the purification of biomolecules.

Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

Abstract: This invention relates to a general method to surface modify porous membranes with a free radical polymerized polymer coating in a layer which is less than the thickness of the membrane. Another object of this invention relates to a general method to surface modify porous membranes with a free radical polymerized polymer coating to a predetermined and controlled depth from a first facial surface. Another object of this invention is to provide a general method to surface modify porous membranes with a free radical polymerized polymer coating into a pattern on one or both facial surfaces, the pattern maintaining its form to a controlled depth into the membrane. Membrane modification is spatially controlled through the use of a polymerization preventing agent (PPA), which prevents modification from occurring in defined spatially volumes of the membrane. The invention relates to porous membranes surface modified by the methods described.

Abstract: Hydrophilic membrane particularly suited for blotting applications, preferably Western blotting. A pre-wet hydrophobic membrane substrate, preferably made of PVDF, is contacted with a monomer solution and subjected to a UV-initiated free radical polymerization step to render the substrate permanently hydrophilic. The resulting membrane exhibits low background fluorescence, high protein binding, excellent retention of protein sample spot morphology, and extended dynamic range (high signal-to-noise ratio, enhanced sample detectability). The membrane demonstrates comparable or higher performance in Western blotting applications than conventional nitrocellulose Western blotting membranes, particularly for protein detection at low sample concentrations, and is directly water-wettable, eliminating the need for an alcohol pre-wet step prior to use.

Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.

Abstract: The present invention provides porous media or membranes having a surface coating that includes a cross-linked terpolymer which has a superior combination of properties, including heat stable biomolecule resistant adsorptive properties, resistance to strong alkaline solutions, and low levels of extractable matter. In some preferred embodiments, the porous media is a porous membrane.

Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.