Abstract: Disclosed are systems and methods for alternately collecting a fluid in a plurality of containers without breaching its sterility. The system generally includes one or more collection modules, each having a valve and one or more container sets fluidically connected to the valve. Each container set includes first and second containers. The system also includes a control unit to control operation of the valve of each collection module to facilitate alternate collection of the fluid by the first and second containers of a container set. This may ensure that the fluid collected in one container is a true representative sample of the fluid collected in the container without compromising its sterile integrity.

Abstract: An aseptic connector includes first and second matable housings each comprising a plurality of connectors for fluid conduits. The fluid conduits enter the housings and may be force-fitted onto barbs or flanges on one side of the connectors, or may instead be molded directly onto the connectors. When the housings are joined, the opposite sides of the connectors mate to form fluid seals, thereby establishing fluid pathways between now-connected sets of tubes.

Abstract: Disclosed are tangential flow filtration (TFF) systems and methods. A system generally includes a TFF module, a control unit and one or more process-control components. In some cases, a system also includes a feed module. The control unit is electrically connected to the one or more process-control components and is configured to control the process of separation using the one or more process-control components. The systems and methods can maintain constant permeate flow rates to improve the predictability or consistence of the process, automatically switch membranes in case of membrane fouling, automatically promote membrane recovery, and/or permit operation of TFF systems in parallel with other processes in a manufacturing production.

Abstract: The invention provides anti-Ara h 2 antibodies (e.g., an anti-Ara h 2 neutralizing antibody) and methods of using the same, e.g., for treating and/or preventing peanut allergy or sensitivity. Also provided herein are anti-Ara h 2 antibodies and methods of using the same, e.g., for diagnostics and methods of monitoring peanut oral immunotherapy.

Abstract: An aseptic connector includes first and second matable housings each comprising a plurality of connectors for fluid conduits. The fluid conduits enter the housings and may be force-fitted onto barbs or flanges on one side of the connectors, or may instead be molded directly onto the connectors. When the housings are joined, the opposite sides of the connectors mate to form fluid seals, thereby establishing fluid pathways between now-connected sets of tubes.

Abstract: A method of tracking cell identity across analytical platforms uses stochastic barcoding (SB). SB uses a randomly generated code based on one or more of the number, color and position of beads encapsulated together with a set of cells of interest. SB use is demonstrated in an application where cells are transferred from a microwell array into a microtiter plate while keeping their identity, and obtained an average identification accuracy of 96% for transfer of 100 blocks. Model scaling of the method up to 1000 blocks demonstrated that SB is able to achieve approximately 90% accuracy.

Abstract: A method of tracking cell identity across analytical platforms uses stochastic barcoding (SB). SB uses a randomly generated code based on one or more of the number, color and position of beads encapsulated together with a set of cells of interest. SB use is demonstrated in an application where cells are transferred from a microwell array into a microtitre plate while keeping their identity, and obtained an average identification accuracy of 96% for transfer of 100 blocks. Model scaling of the method up to 1000 blocks demonstrated that SB is able to achieve approximately 90% accuracy.

Abstract: The present disclosure provides technologies for achieving cell patterning, as well as patterned cell arrays achieved with such technologies. Provided apparatus and methods are useful in a variety of contexts and provide particular advantages with respect to assessing living cell arrays and/or their responsiveness to stimuli, including identifying and/or characterizing complex cellular responses.

Abstract: A system for providing fully automated image segmentation and quality control of a protein array with no human interaction is presented. The system includes a memory and a processor configured by the memory. The processor is configured to receive an image file of the protein array, to geometrically distinguish a first block within the image, register a nominal location of the first block to the image by assigning a geometric code identifying the first block, draw a plurality of grid line on the array to delineate the first block from a second block, iteratively cluster a sub image for each feature in the delineated blocks to define a foreground area and a background area, identify pixels containing an artifact for each feature, and extract and return the signal intensity, variance, and quality of background and foreground pixels in each feature.

Abstract: A microarray formed in a planar surface of a moldable slab, the microarray including a plurality of microwell sets comprising a plurality of microwells formed in the planar surface of the moldable slab, each microwell being sized to contain at least a single cell, and a plurality of microchannels formed in the planar surface of the moldable slab, the plurality of microchannels being configured to permit liquid from a first region of the microarray to transit to a second region of the microarray.

Abstract: A microarray formed in a planar surface of a moldable slab, the microarray including a plurality of microwell sets comprising a plurality of microwells formed in the planar surface of the moldable slab, each microwell being sized to contain at least a single cell, and a plurality of microchannels formed in the planar surface of the moldable slab, the plurality of microchannels being configured to permit liquid from a first region of the microarray to transit to a second region of the microarray.

Abstract: A monomolecular rectifying wire is provided that includes a molecular conducting wire that has been modified to embed a rectifying diode integrally therein. The modified conducting wire includes an electron donating section, an electron accepting section, and an insulating group separating the electron donating and accepting sections. Monomolecular logical gates and more complex logic structures that operate by the conduction of electrical currents are constructed by combinations of molecular rectifying wires, molecular conducting wires, and molecular resistive wires to form a single molecule that electrically exhibits a desired Boolean logic function.

Abstract: The present invention of compounds of formula (I), ##STR1## a stereochemically isomeric form thereof, an N-oxide form thereof or a pharmaceutically acceptable acid addition salt thereof, R.sup.1 is C.sub.1-6 alkyloxy, C.sub.2-6 alkenyloxy or C.sub.2-6 alkynyloxy; R.sup.2 is hydrogen or C.sub.1-6 alkyloxy, or when taken together R.sup.1 and R.sup.2 may form a bivalent radical of formula wherein in said bivalent radicals one or two hydrogen atoms may be substituted with C.sub.1-6 alkyl; R.sup.3 is hydrogen or halo; R.sup.4 is hydrogen or C.sub.1-6 alkyl; L is C.sub.3-6 cycloalkyl, C.sub.5-6 cycloalkanone, C.sub.2-6 alkenyl optionally substituted with aryl, or L is a radical of formula -Alk-R.sup.5 -, Alk-X--R.sup.6, -Alk-Y--C(.dbd.O)--R.sup.8, or -Alk-Y--C(.dbd.O)--NR.sup.10 R.sup.11 wherein each Alk is C.sub.1-12 alkanediyl; and R.sup.5 is hydrogen, cyano, C.sub.1-6 alkylsulfonylamino, C.sub.3-6 cycloalkyl, C.sub.5-6 cycloalkanone, aryl, di(aryl)methyl or a heterocyclic ringsystem; R.sup.6 is hydrogen, C.sub.