Abstract: A boom assembly for a demolition machine includes a main support having a support cylinder configured to raise and lower the main support, an outer boom arm attached to the main support, an inner boom arm received within the outer boom arm, the inner boom arm axially moveable relative to the outer boom arm via an inner boom cylinder, and a hydraulically actuated tool implement. Hydraulic lines for the hydraulically actuated tool implement are received within the inner boom arm.

Abstract: A demolition machine includes a deck having a front edge, a rear edge, a left side, and a right side, with the deck defining a central axis along a direction extending from the rear edge to the front edge and equally spaced from the left side and the right side of the deck, a drive assembly secured to the deck, with the drive assembly rotatable relative to the deck, an engine received by the deck and positioned at one of the left side and right side of the deck, an energy reservoir and hydraulic reservoir each received by the deck and positioned on an opposite side of the deck from the engine, and a boom assembly secured to a boom support structure of the deck. The boom assembly is moveable relative to the deck, with the boom support structure positioned on the central axis.

Abstract: A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dNTPs according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dNTPs according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

Abstract: Herein is disclosed a method for ink or coating density compensation by means of applying calculated additional ink/coating to the pre-decorated substrate or film, and by additionally, if needed, apply colour-correction to pre-decoration print files to compensate for higher-than-desired ink density colour shifts. The method includes the steps of providing a flat polymer web having a uniform grid pattern of grid markers printed thereon, and then thermally transforming the web to form a three-dimensional target grid. The target grid is then digitized and the change in the spacing of the grid markers is measured and recorded. The recorded transformed spacing for each grid marker is then used to modify the initial graphics file to compensate for ink-density irregularities resulting from the shrinking and stretching deformations of the substrate during thermal transformation.

Abstract: Herein is disclosed a method for ink or coating density compensation by means of applying calculated additional ink/coating to the pre-decorated substrate or film, and by additionally, if needed, apply colour-correction to pre-decoration print files to compensate for higher-than-desired ink density colour shifts. The method includes the steps of providing a flat polymer web having a uniform grid pattern of grid markers printed thereon, and then thermally transforming the web to form a three-dimensional target grid. The target grid is then digitized and the change in the spacing of the grid markers is measured and recorded. The recorded transformed spacing for each grid marker is then used to modify the initial graphics file to compensate for ink-density irregularities resulting from the shrinking and stretching deformations of the substrate during thermal transformation.

Abstract: A demolition machine includes a deck having a front edge, a rear edge, a left side, and a right side, with the deck defining a central axis along a direction extending from the rear edge to the front edge and equally spaced from the left side and the right side of the deck, a drive assembly secured to the deck, with the drive assembly rotatable relative to the deck, an engine received by the deck and positioned at one of the left side and right side of the deck, an energy reservoir and hydraulic reservoir each received by the deck and positioned on an opposite side of the deck from the engine, and a boom assembly secured to a boom support structure of the deck. The boom assembly is moveable relative to the deck, with the boom support structure positioned on the central axis.

Abstract: A method for transforming a two-dimensional graphic image into a two-dimensional distorted image which can then be used to recreate an accurate representation of the two-dimensional graphic image when applied onto a 3D surface. The method includes the steps of producing and identifying a target 3D grid from a plurality of flat polymer webs each having a grid pattern printed thereon, the target 3D grid bearing an accurate representation of the 3D surface. The three-dimensional shape of the target grid is then digitized to form a geometric model of the target grid as a series of data points. Creating a uniform smooth 3D model from the series of data points and then texture mapping the two-dimensional graphic image onto the uniform smooth 3D model to create a texture map. The two-dimensional distorted image is then created from the texture map and the two-dimensional graphic image.

Abstract: A method for transforming a two-dimensional graphic image into a two-dimensional distorted image which can then be used to recreate an accurate representation of the two-dimensional graphic image when applied onto a 3D surface. The method includes the steps of producing and identifying a target 3D grid from a plurality of flat polymer webs each having a grid pattern printed thereon, the target 3D grid bearing an accurate representation of the 3D surface. The three-dimensional shape of the target grid is then digitized to form a geometric model of the target grid as a series of data points. Creating a uniform smooth 3D model from the series of data points and then texture mapping the two-dimensional graphic image onto the uniform smooth 3D model to create a texture map. The two-dimensional distorted image is then created from the texture map and the two-dimensional graphic image.

Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Abstract: A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dNTPs according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dNTPs according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.

Abstract: In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such embodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.

Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Abstract: A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dNTPs according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dNTPs according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.

Abstract: In some embodiments, the disclosure relates generally to methods as well as related compositions, systems, kits and apparatus comprising linking proteins to target compounds and/or to locations of interest using tethers. For example, the tether can be used to link the protein to a target compound, for example, to link an enzyme to a substrate. Similarly, the tether can be used to link the protein at or near a desired location on a surface. In one group of embodiments, the tether includes a polynucleotide and the target compound or location on the surface includes another polynucleotide that is capable of hybridizing to the tether. In such embodiments, the tether can be used to link the protein to the target compound or location using nucleic acid hybridization.

Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Abstract: Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions.