Abstract: A method of sequencing a polynucleotide strand can include providing the polynucleotide strand with a primer annealed thereto and a polymerase operably bound to the polynucleotide strand; successively exposing the polynucleotide strand to the flow of four different dNTPs according to a first predetermined ordering; and successively exposing the polynucleotide strand to the flow of four different dNTPs according to a second predetermined ordering, wherein the second predetermined ordering is different from the first predetermined ordering.

Abstract: A computer-implemented method of differentiating between lymphoid blast cells and myeloid blast cells comprises: receiving a digital image containing one or more blast cells; applying a parametric model classifier to one or more portions of the digital image each containing a respective blast cell, the parametric model configured to generate an output indicative of whether each blast cell is a lymphoid blast cell or a myeloid blast cell. Computer-implemented methods of training a parametric model are also provided, as well as a clinical decision support system relying on the computer-implemented method of classifying blast cells.

Abstract: A method for making a laminated glazing panel is described. A bolt having a head portion and a stem portion extending therefrom is positioned in a hole in a sheet of glazing material so that a first surface of the head portion is faces in the direction of a first major surface of the sheet of glazing material and the stem portion extends beyond a second major surface of the sheet of glazing material. A sheet of adhesive interlayer material is positioned on the first major surface of the sheet of glazing material to cover the first surface of the head portion. Suitable lamination conditions are used to laminate the sheet of adhesive interlayer material to the sheet of glazing material. During lamination, fluid trapped between the first surface of the head portion and the sheet of adhesive interlayer material is removed via a fluid pathway associated with the stem portion. Laminated glazing panels made using the method are also described.

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

Abstract: A system for supplementing measurement results of automated analyzers is presented. The system includes a computer device configured for obtaining a result of a measurement performed by an automated analyzer, the computer device and the automated analyzer being located within a privileged computer network, obtaining a context related algorithm associated with the result of the measurement defining one or more triggering conditions and context related information from a computer device residing outside of the privileged computer network at the computer device and processing the result of the measurement by using the context related algorithm to generate a context specific supplement to the result of the measurement at the computer device.

Abstract: Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.

Abstract: A laminated glazing panel comprising first and second sheets of glazing material joined by an interlayer structure comprising a first sheet of adhesive interlayer material is described. Inboard of a peripheral edge of the first sheet of glazing material, the first sheet of glazing material has a hole therein. The laminated glazing panel further comprises a glazing fitting secured thereto, the glazing fitting comprising a head portion and an attachment feature. The head portion is positioned in the hole such that a first surface thereof faces the first sheet of adhesive interlayer material. Some of the first sheet of adhesive interlayer material is in the hole and is operatively associated with the first attachment feature thereby attaching the glazing fitting to the first sheet of adhesive interlayer material. Suitable glazing fittings and methods of making such laminated glazing panels are also described.

Abstract: Herein is disclosed a method for ink or coating density compensation by means of applying calculated additional ink/coating to the pre-decorated substrate or film, and by additionally, if needed, apply colour-correction to pre-decoration print files to compensate for higher-than-desired ink density colour shifts. The method includes the steps of providing a flat polymer web having a uniform grid pattern of grid markers printed thereon, and then thermally transforming the web to form a three-dimensional target grid. The target grid is then digitized and the change in the spacing of the grid markers is measured and recorded. The recorded transformed spacing for each grid marker is then used to modify the initial graphics file to compensate for ink-density irregularities resulting from the shrinking and stretching deformations of the substrate during thermal transformation.

Abstract: Herein is disclosed a method for ink or coating density compensation by means of applying calculated additional ink/coating to the pre-decorated substrate or film, and by additionally, if needed, apply colour-correction to pre-decoration print files to compensate for higher-than-desired ink density colour shifts. The method includes the steps of providing a flat polymer web having a uniform grid pattern of grid markers printed thereon, and then thermally transforming the web to form a three-dimensional target grid. The target grid is then digitized and the change in the spacing of the grid markers is measured and recorded. The recorded transformed spacing for each grid marker is then used to modify the initial graphics file to compensate for ink-density irregularities resulting from the shrinking and stretching deformations of the substrate during thermal transformation.

Abstract: A laminated glazing panel comprising first and second sheets of glazing material joined by an interlayer structure comprising a first sheet of adhesive interlayer material is described. Inboard of a peripheral edge of the first sheet of glazing material, the first sheet of glazing material has a hole therein. The laminated glazing panel further comprises a glazing fitting secured thereto, the glazing fitting comprising a head portion and an attachment feature. The head portion is positioned in the hole such that a first surface thereof faces the first sheet of adhesive interlayer material. Some of the first sheet of adhesive interlayer material is in the hole and is operatively associated with the first attachment feature thereby attaching the glazing fitting to the first sheet of adhesive interlayer material. Suitable glazing fittings and methods of making such laminated glazing panels are also described.

Abstract: A method for making a laminated glazing panel is described. A bolt having a head portion and a stem portion extending therefrom is positioned in a hole in a sheet of glazing material so that a first surface of the head portion is faces in the direction of a first major surface of the sheet of glazing material and the stem portion extends beyond a second major surface of the sheet of glazing material. A sheet of adhesive interlayer material is positioned on the first major surface of the sheet of glazing material to cover the first surface of the head portion. Suitable lamination conditions are used to laminate the sheet of adhesive interlayer material to the sheet of glazing material. During lamination, fluid trapped between the first surface of the head portion and the sheet of adhesive interlayer material is removed via a fluid pathway associated with the stem portion. Laminated glazing panels made using the method are also described.

Abstract: A demolition machine includes a deck having a front edge, a rear edge, a left side, and a right side, with the deck defining a central axis along a direction extending from the rear edge to the front edge and equally spaced from the left side and the right side of the deck, a drive assembly secured to the deck, with the drive assembly rotatable relative to the deck, an engine received by the deck and positioned at one of the left side and right side of the deck, an energy reservoir and hydraulic reservoir each received by the deck and positioned on an opposite side of the deck from the engine, and a boom assembly secured to a boom support structure of the deck. The boom assembly is moveable relative to the deck, with the boom support structure positioned on the central axis.

Abstract: A method for transforming a two-dimensional graphic image into a two-dimensional distorted image which can then be used to recreate an accurate representation of the two-dimensional graphic image when applied onto a 3D surface. The method includes the steps of producing and identifying a target 3D grid from a plurality of flat polymer webs each having a grid pattern printed thereon, the target 3D grid bearing an accurate representation of the 3D surface. The three-dimensional shape of the target grid is then digitized to form a geometric model of the target grid as a series of data points. Creating a uniform smooth 3D model from the series of data points and then texture mapping the two-dimensional graphic image onto the uniform smooth 3D model to create a texture map. The two-dimensional distorted image is then created from the texture map and the two-dimensional graphic image.

Abstract: A method for transforming a two-dimensional graphic image into a two-dimensional distorted image which can then be used to recreate an accurate representation of the two-dimensional graphic image when applied onto a 3D surface. The method includes the steps of producing and identifying a target 3D grid from a plurality of flat polymer webs each having a grid pattern printed thereon, the target 3D grid bearing an accurate representation of the 3D surface. The three-dimensional shape of the target grid is then digitized to form a geometric model of the target grid as a series of data points. Creating a uniform smooth 3D model from the series of data points and then texture mapping the two-dimensional graphic image onto the uniform smooth 3D model to create a texture map. The two-dimensional distorted image is then created from the texture map and the two-dimensional graphic image.

Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Abstract: The present invention is a water purification system and method including a treatment tank with a water inlet, a chlorine source, and a heating element; at least one storage tank with at least one treated water outlet between the treatment tank and the storage tank(s) through which treated water passes therebetween, and a final water outlet(s) through which treated water leaves the system for the benefit of the end user. A power source powers all elements that require power, such as the heating element.

Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Abstract: The present invention is a water purification system and method including a treatment tank with a water inlet, a chlorine source, and a heating element; at least one storage tank with at least one treated water outlet between the treatment tank and the storage tank(s) through which treated water passes therebetween, and a final water outlet(s) through which treated water leaves the system for the benefit of the end user. A power source powers all elements that require power, such as the heating element.

Abstract: A fitting (100), for securing an elongate member to a support structure, at least substantially consisting of filamentary material. The fitting (100) is shaped to define closed bottom portions (122), (124); a front portion (110) having sides (162), (164) that are mutually connected by one bottom portion (122); a back portion (130) having sides (182), (184) that are mutually connected by another bottom portion (124); a closed top portion (142) connecting a side (162) of the front portion (110) to a side (182) of the back portion (130); and another closed top portion (144) connecting another side (164) of the front portion (110) to another side (184) of the back portion (130). The front portion (110) is spaced from the back portion (130) to define a receiving space for receiving the elongate member. The front portion (110) is in register with the back portion (130) such that the front portion (110) and the back portion (130) are skewerable by a fastener engageable with the support structure.

Abstract: The present invention is a water purification system and method including a treatment tank with a water inlet, a chlorine source, and a heating element; at least one storage tank with at least one treated water outlet between the treatment tank and the storage tank(s) through which treated water passes therebetween, and a final water outlet(s) through which treated water leaves the system for the benefit of the end user. A power source powers all elements that require power, such as the heating element.