Abstract: The present invention provides, in part, protein-drug conjugates comprising an anti-transferrin receptor (e.g., human transferrin receptor) antigen-binding protein (e.g., scFv, Fab) conjugated to a molecular cargo (e.g., polynucleotides, liposomes or lipid nanoparticles) for delivery of the molecular cargo to a targeted tissue (e.g., brain or muscle). Methods for treating various diseases or disorders, such as neurological diseases or muscular diseases, with the conjugates are provided.

Abstract: Provided herein are compositions comprising a nucleic acid encoding amyloid-beta precursor protein and/or a nucleic acid encoding presenilin-1, cells comprising the compositions, animals comprising the compositions, methods of making the cells and animals, methods of modeling Alzheimer's disease, methods of assessing a therapeutic candidate for the treatment of Alzheimer's disease or amelioration of a symptom or phenotype of Alzheimer's disease, and methods of assessing a therapeutic candidate for the prevention of Alzheimer's disease or prevention of a symptom or phenotype of Alzheimer's disease.

Abstract: A nozzle assembly includes a nozzle, a manifold, and wand body. The nozzle, manifold, and wand body can be coupled together to provide a robust spray drying system that can withstand elevated temperature and/or pressure feed stock. In some embodiments, an internal shoulder portion of the manifold can engage with a side of a nozzle collar to restrict distal movement of the nozzle relative to the manifold.

Abstract: Provided, in part, are an anti-human transferrin receptor antigen-binding proteins and fusion proteins comprising an anti-human transferrin receptor antigen-binding proteins (e.g., in scFv, Fab or antibody format) which may be fused to a payload for delivery of the payload to a targeted tissue (e.g., past the blood-brain barrier and to the brain). Payloads include, for example, alpha-glucosidase (GAA) polypeptide. Methods for treating various diseases with such molecules, e.g., glycogen storage diseases, such as Pompe Disease, with the fusions are provided.

Abstract: Nucleic acid constructs and compositions that allow insertion of a multidomain therapeutic protein (e.g., GAA fusion protein) coding sequence into a target genomic locus such as an endogenous ALB locus and/or expression of the multidomain therapeutic protein (e.g., GAA fusion protein) coding sequence are also provided. The nucleic acid constructs and compositions can be used in methods of integration of a multidomain therapeutic protein (e.g., GAA fusion protein) nucleic acid into a target genomic locus, methods of expression of a multidomain therapeutic protein (e.g., GAA fusion protein) in a cell, methods of reducing glycogen accumulation, methods of treating Pompe disease or GAA deficiency in a subject, and method of preventing or reducing the onset of a sign or symptom of Pompe disease in a subject, including neonatal cells and subjects.

Abstract: A nozzle assembly includes a nozzle, a manifold, and wand body. The nozzle, manifold, and wand body can be coupled together to provide a robust spray drying system that can withstand elevated temperature and/or pressure feed stock. In some embodiments, an internal shoulder portion of the manifold can engage with a side of a nozzle collar to restrict distal movement of the nozzle relative to the manifold.

Abstract: A beverage container having a slidably openable lid assembly is disclosed. The lid assembly basically comprises a base member and a cover member. The base member includes a wall portion releasably securing the lid assembly to the container and an opening in fluid communication with a beverage holding chamber in the container. The cover member is slidably coupled to the base member to slide from a closed position to an open position and vice versa. When the cover member is in the closed position it isolates the opening and the contiguous portions of the base member from the ambient surroundings. When the cover member is in the open position it exposes the opening to enable a user to drink the beverage via the opening. The cover includes an elastomeric member arranged to engage and seal the opening in the base member when the cover member is in the closed position.

Abstract: A beverage container having a slidably openable lid assembly is disclosed. The lid assembly basically comprises a base member and a cover member. The base member includes a wall portion releasably securing the lid assembly to the container and an opening in fluid communication with a beverage holding chamber in the container. The cover member is slidably coupled to the base member to slide from a closed position to an open position and vice versa. When the cover member is in the closed position it isolates the opening and the contiguous portions of the base member from the ambient surroundings. When the cover member is in the open position it exposes the opening to enable a user to drink the beverage via the opening. The cover includes an elastomeric member arranged to engage and seal the opening in the base member when the cover member is in the closed position.

Abstract: A beverage container having a slidably openable lid assembly is disclosed. The lid assembly basically comprises a base member and a cover member. The base member includes a wall portion releasably securing the lid assembly to the container and an opening in fluid communication with a beverage holding chamber in the container. The cover member is slidably coupled to the base member to slide from a closed position to an open position and vice versa. When the cover member is in the closed position it isolates the opening and the contiguous portions of the base member from the ambient surroundings. When the cover member is in the open position it exposes the opening to enable a user to drink the beverage via the opening. The cover includes an elastomeric member arranged to engage and seal the opening in the base member when the cover member is in the closed position.

Abstract: To estimate water depth, photos of shoaling ocean waves are analyzed in view of primary wave frequency and length, mean water level, and known error sources. Primary wave frequency and mean water level are found from independent surface-wave and tide-level hindcasting systems for the time and place of the photos. Primary wave length is calculated from the two-dimensional Fourier transform for various subregion sizes and maximizing the peak value as a function of subregion size. Error is reduced by analysis of the above parameters and also the noise of higher-frequency wind waves and other image artifacts such as fronts and slicks. Error residuals are reduced through statistical analysis, again using independent images. Robustness is significantly enhanced by collecting images on different days.