Abstract: Aspects of the invention relate to methods of producing small molecules for industrial application using natural organisms and engineered organisms.

Abstract: The invention relates to methods of producing eukaryotic cell libraries encoding a repertoire of binding molecules (“binders”), wherein the methods use a site-specific nuclease for targeted cleavage of cellular DNA to enhance site-specific integration of binder genes through endogenous cellular repair mechanisms. Populations of eukaryotic cells are produced in which a repertoire of genes encoding binders are integrated into a desired locus in cellular DNA (e.g., a genomic locus) allowing expression of the encoded binding molecule, thereby creating a population of cells expressing different binders.

Abstract: The invention relates to methods of producing eukaryotic cell libraries encoding a repertoire of binding molecules (“binders”), wherein the methods use a site-specific nuclease for targeted cleavage of cellular DNA to enhance site-specific integration of binder genes through endogenous cellular repair mechanisms. Populations of eukaryotic cells are produced in which a repertoire of genes encoding binders are integrated into a desired locus in cellular DNA (e.g., a genomic locus) allowing expression of the encoded binding molecule, thereby creating a population of cells expressing different binders.

Abstract: In general, the present invention is directed to systems for treating water or wastewater. In accordance with some embodiments of the present invention, the system may utilize a vessel with a plurality of filter beds, at least one inlet, and at least one outlet, and the system may include: a first filtration bed comprising a first granular and/or angular media, the first media having a high surface area and configured for biological and physical treatment of the water or wastewater; a second filtration bed including a second granular and/or angular media, the second media having a lower surface area than the first media; wherein the water or wastewater enters the vessel via the at least one inlet, flows through the first filtration bed and the second filtration bed, and exits the vessel through the at least one outlet.

Abstract: An air traffic control system, for use by a controller controlling multiple aircraft on landing or takeoff on a runway, comprising a processor, an input device and a display device, in which the separation between a first aircraft and a second aircraft immediately following it on the runway is determined taking into account the type of the first aircraft and its consequent impact on the landing beams used in poor visibility.

Abstract: In general, the present invention is directed to systems for treating water or wastewater. In accordance with some embodiments of the present invention, the system may utilize a vessel with a plurality of filter beds, at least one inlet, and at least one outlet, and the system may include: a first filtration bed comprising a first granular and/or angular media, the first media having a high surface area and configured for biological and physical treatment of the water or wastewater; a second filtration bed including a second granular and/or angular media, the second media having a lower surface area than the first media; wherein the water or wastewater enters the vessel via the at least one inlet, flows through the first filtration bed and the second filtration bed, and exits the vessel through the at least one outlet.

Abstract: The device of the present invention comprises an elongated tube which is adapted to be inserted into the body so that its internal end is positioned adjacent the tissue which is to be sutured. A plunger is sized to slide within the tube and includes at one end a grasping mechanism for releaseably grasping a pair of needles in spaced-apart relation to one another. The plunger is fitted within the tube and the needles are forced outwardly through the lower end of the tube so that they penetrate and extend through the tissue to be sutured. The needles are forced through the tissue to be sutured and are forced outwardly through the skin layer of the patient so that they can be grasped and pulled from the releaseable grasping mechanism of the plunger.