Patents by Inventor John Dzenitis
John Dzenitis has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11779925Abstract: Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.Type: GrantFiled: December 13, 2021Date of Patent: October 10, 2023Assignee: Bio-Rad Laboratories, Inc.Inventors: Thomas Henry Cauley, III, John Dzenitis, Steve Hobbs, Stefano Schiaffino
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Publication number: 20230070234Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.Type: ApplicationFiled: November 14, 2022Publication date: March 9, 2023Applicant: Bio-Rad Laboratories, Inc.Inventors: David P. STUMBO, George CARMAN, Steve HOBBS, Anthony J. MAKAREWICZ, JR., Dmitri SIMONIAN, David GLADE, Joshua OEN, Denis PRISTINSKI, John DZENITIS
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Patent number: 11499183Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.Type: GrantFiled: June 28, 2018Date of Patent: November 15, 2022Assignee: Bio-Rad Laboratories, Inc.Inventors: David P. Stumbo, George Carman, Steve Hobbs, Anthony J. Makarewicz, Jr., Dmitri Simonian, David Glade, Joshua Oen, Denis Pristinski, John Dzenitis
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Publication number: 20220097068Abstract: Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.Type: ApplicationFiled: December 13, 2021Publication date: March 31, 2022Applicant: Bio-Rad Laboratories, Inc.Inventors: Thomas Henry CAULEY, III, John DZENITIS, Steve HOBBS, Stefano SCHIAFFINO
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Patent number: 11207685Abstract: Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.Type: GrantFiled: February 12, 2018Date of Patent: December 28, 2021Assignee: Bio-Rad Laboratories, Inc.Inventors: Thomas Henry Cauley, III, John Dzenitis, Steve Hobbs, Stefano Schiaffino
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Publication number: 20190002956Abstract: Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel.Type: ApplicationFiled: June 28, 2018Publication date: January 3, 2019Inventors: David P. STUMBO, George CARMAN, Steve HOBBS, Anthony J. MAKAREWICZ, JR., Dmitri SIMONIAN, David GLADE, Joshua OEN, Denis PRISTINSKI, John DZENITIS
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Publication number: 20180229239Abstract: Systems, methods, and devices for forming an array of emulsions. An exemplary device comprises a frame and at least one or a plurality of separate microfluidic modules mounted to the frame and each configured to form an array of emulsions. In some embodiments, each module may be mounted by snap-fit attachment. The device also may include the same sealing member bonded to a top side of each module and hermetically sealing each of the modules. Another exemplary microfluidic device for forming an array of emulsions comprises a stack of layers bonded together. The stack may comprise a port layer forming a plurality of ports. Each port may have a top rim formed by a protrusion that encircles the central axis of the port. The rims may be coplanar with one another to facilitate bonding of a sealing member to each rim.Type: ApplicationFiled: February 12, 2018Publication date: August 16, 2018Inventors: Trey CAULEY, John DZENITIS, Steve HOBBS, Stefano SCHIAFFINO
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Publication number: 20180209874Abstract: An assay performance system may include modules configured to store aqueous sample plates, conduct droplet generation or emulsification of aqueous samples, and to perform thermocycling and droplet reading functions. One or more samples may be emulsified and stored in an emulsified state for extended times prior to thermocycling. Accordingly, the assay performance system may include material handling systems and methods to accommodate the storage function.Type: ApplicationFiled: January 26, 2018Publication date: July 26, 2018Inventors: Sean Cater, John Dzenitis, Stefano Schiaffino, Pallavi Shah, Andy Utada
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Publication number: 20170189908Abstract: The present disclosure provides systems and methods for performing droplet assays with automatic calibration. An exemplary assay system may comprise a cartridge including a plurality of droplet generators to form emulsions of droplets having a same nominal volume. A tag may be associated with the cartridge and may encode calibration data or an identifier thereof. The calibration data may include a respective value specific to each droplet generator. The system further may include a detection system to detect a signal representing an analyte from droplets of each emulsion, and a reader to read the calibration data or the identifier from the tag. The system still further may include a processor configured to receive the signal and the calibration data and to calculate, for each emulsion, a concentration of an analyte using at least the signal and the respective value specific to the droplet generator that formed the emulsion.Type: ApplicationFiled: December 30, 2016Publication date: July 6, 2017Inventors: John Dzenitis, Stefano Schiaffino
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Publication number: 20060281101Abstract: A bio-briefcase system for analyzing a sample for the presence of biological agents. The bio-briefcase system comprises a housing, an immunoassay section operatively connected to the housing, and/or a nucleic acid assay section operatively connected to the housing.Type: ApplicationFiled: June 15, 2005Publication date: December 14, 2006Inventors: John Dzenitis, William Benett, Raymond Mariella, Steven Visuri, Kodumudi Venkateswaran
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Publication number: 20060057599Abstract: An autonomous monitoring apparatus for monitoring air, water, soil, or other substance for bioagents. A collector gathers a quantity of the air, water, soil, or other substance being monitored. A sample preparation system prepares a sample of the selected potential bioagent particles. The sample is analyzed by a system for detecting said bioagents.Type: ApplicationFiled: November 29, 2004Publication date: March 16, 2006Inventors: John Dzenitis, Steve Brown, Billy Colston, Dora Gutierrez, Bruce Henderer, Benjamin Hindson, Ramakrishna Madabhushi, Anthony Makarewicz, Don Masquelier, Mary McBride, Thomas Metz, Shanavaz Nasarabadi, Ujwal Setlur, Sally Smith, Kodumudi Venkateswaran
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Publication number: 20050059029Abstract: A method of making very long, double-stranded synthetic poly-nucleotides. A multiplicity of short oligonucleotides is provided. The short oligonucleotides are sequentially hybridized to each other. Enzymatic ligation of the oligonucleotides provides a contiguous piece of PCR-ready DNA of predetermined sequence.Type: ApplicationFiled: November 21, 2003Publication date: March 17, 2005Inventors: Raymond Mariella, Allen Christian, James Tucker, John Dzenitis, Alexandros Papavasiliou
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Publication number: 20050029308Abstract: A dispensing system delivers a precise amount of fluid for biological or chemical processing and/or analysis. Dispensing means moves the fluid. The dispensing means is operated by a pneumatic force. Connection means delivers the fluid to the desired location. An actuator means provides the pneumatic force to the dispensing means. Valving means transmits the pneumatic force from the actuator means to the dispensing means.Type: ApplicationFiled: July 16, 2003Publication date: February 10, 2005Inventors: William Benett, Peter Krulevitch, Steven Visuri, John Dzenitis, Kevin Ness