Abstract: Provided herein are systems, methods, and cryoprotective solutions for reversible cryopreservation of biological specimens, whole organs, and whole organisms. Exemplary methods include loading a cryoprotective agent into the biological specimen, cooling the biological specimen to a cryogenic temperature for preservation, storing the biological specimen at a preservation temperature state to preserve the biological specimen, rewarming the biological specimen by increasing a temperature of the biological specimen above the preservation temperature state, and unloading the cryoprotective agent from the biological specimen. The cooling is performed at a first rate to reduce ice formation, substantially homogeneously to reduce propensity for cracking of the preserved biological specimen, and at a first pressure to prevent or reduce ice expansion within the preserved biological specimen.

Abstract: Provided herein are systems, methods, and cryoprotective solutions for reversible cryopreservation of biological specimens, whole organs, and whole organisms. Exemplary methods include loading a cryoprotective agent into the biological specimen, cooling the biological specimen to a cryogenic temperature for preservation, storing the biological specimen at a preservation temperature state to preserve the biological specimen, rewarming the biological specimen by increasing a temperature of the biological specimen above the preservation temperature state, and unloading the cryoprotective agent from the biological specimen. The cooling is performed at a first rate to reduce ice formation, substantially homogeneously to reduce propensity for cracking of the preserved biological specimen, and at a first pressure to prevent or reduce ice expansion within the preserved biological specimen.

Abstract: A method and system for prioritizing, securing, and reducing the amount of data transmitted and stored during the creation of a backup copy of file data. Sectors in which changes have been made are identified as are the actual changes made to the sectors. Only the actual changes within each changed sector, along with the HCS number and a byte offset identifying the location within the sector at which the changes occur, are transmitted to the backup site. Files that are to be transmitted to the backup site are prioritized according to ratings based on predetermined criteria. Higher rated files are transmitted to the backup site prior to lower rated files. The files that are to be transmitted to the backup site are encoded and double encrypted. All instances of predetermined client-specific data elements within each file are identified and replaced by a corresponding code prior to encryption.