Patents by Inventor John E. Blume

John E. Blume has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods and computer software for detecting splice variants
    Publication number: 20110208500
    Abstract: Methods and software products for analysis of alternative splicing are disclosed. In general the methods involve normalizing probe set or exon intensity to an expression level measurement of the gene. The methods may be used to identify tissue-specific alternative splicing events.
    Type: Application
    Filed: May 4, 2011
    Publication date: August 25, 2011
    Applicant: AFFYMETRIX, INC.
    Inventors: Alan Williams, Simon Cawley, John E. Blume, Hui Wang, Tyson Clark
  • Analysis of methylation using nucleic acid arrays
    Publication number: 20110166037
    Abstract: Arrays for genome-wide analysis of methylation are disclosed. IN a preferred aspect arrays comprising a plurality of probes complementary to a plurality of identified CpG islands in the human, mouse and rat genome are disclosed. The arrays may be used to detect methylation within cpG islands in samples from human, mouse and rat genomes.
    Type: Application
    Filed: January 27, 2011
    Publication date: July 7, 2011
    Applicant: AFFYMETRIX, INC.
    Inventors: Yanxiang Cao, Shivani Nautiyal, Charles G. Miyada, Christopher Davies, Gangwu Mei, Alan J Williams, Eric B. Schell, John E. Blume
  • Methods and computer software for detecting splice variants
    Patent number: 7962291
    Abstract: Methods and software products for analysis of alternative splicing are disclosed. In general the methods involve normalizing probe set or exon intensity to an expression level measurement of the gene. The methods may be used to identify tissue-specific alternative splicing events.
    Type: Grant
    Filed: October 2, 2006
    Date of Patent: June 14, 2011
    Assignee: Affymetrix, Inc.
    Inventors: Alan Williams, Simon Cawley, John E. Blume, Hui Wang, Tyson Clark
  • Analysis of methylation using nucleic acid arrays
    Patent number: 7901882
    Abstract: Arrays for genome-wide analysis of methylation are disclosed. In a preferred aspect arrays comprising a plurality of probes complementary to a plurality of identified CpG islands in the human, mouse and rat genome are disclosed. The arrays may be used to detect methylation within CpG islands in samples from human, mouse and rat genomes.
    Type: Grant
    Filed: April 2, 2007
    Date of Patent: March 8, 2011
    Assignee: Affymetrix, Inc.
    Inventors: Yanxiang Cao, Shivani Nautiyal, Charles G. Miyada, Christopher Davies, Gangwu Mei, Alan J. Williams, Eric B. Schell, John E. Blume
  • DNA LIGATION ON RNA TEMPLATE
    Publication number: 20100184618
    Abstract: Disclosed are methods and compositions for detection and amplification of nucleic acids, wherein two DNA strands hybridized to an RNA strand are ligated. In one aspect, the disclosed methods include removal of an energy source, such as ATP, upon charging a ligase to form an enzyme-AMP intermediate, and then adding substrate, which results in one complete round of RNA-templated DNA ligation. In another aspect, the ligation reaction is accomplished by use of a mixture of at least two different ligase enzymes. The disclosed methods and compositions for RNA-templated DNA ligation may be particularly useful for detection of RNA sequence variants, for example RNA splice variants, and for quantitative expression analysis.
    Type: Application
    Filed: January 19, 2010
    Publication date: July 22, 2010
    Applicant: AFFYMETRIX, INC.
    Inventors: EUGENI A. NAMSARAEV, XIN MIAO, JOHN E. BLUME
  • Polynucleotides encoding an isolated ?-islet cell two-pore domain potassium channel
    Patent number: 7491533
    Abstract: The present invention provides polynucleotides encoding potassium channels as well as methods of using the potassium channels to treat and diagnose diabetes and a predisposition for diabetes.
    Type: Grant
    Filed: March 3, 2006
    Date of Patent: February 17, 2009
    Assignee: Metabolex, Inc.
    Inventors: Jeffrey D. Johnson, John F. Palma, Anthony C. Schweitzer, John E. Blume
  • PANCREATIC ISLET TRANSCRIPTION FACTOR AND USES THEREOF
    Publication number: 20080318312
    Abstract: The present invention provides a pancreatic islet transcription factor and methods of treating and diagnosing diabetes.
    Type: Application
    Filed: June 27, 2008
    Publication date: December 25, 2008
    Applicant: METABOLEX, INC.
    Inventors: Jeffrey D. Johnson, John F. Palma, Anthony C. Schweitzer, John E. Blume
  • Pancreatic islet transcription factor and uses thereof
    Patent number: 7405289
    Abstract: The present invention provides a pancreatic islet transcription factor and methods of treating and diagnosing diabetes.
    Type: Grant
    Filed: November 13, 2003
    Date of Patent: July 29, 2008
    Assignee: Metabolex, Inc.
    Inventors: Jeffrey D. Johnson, John F. Palma, Anthony C. Schweitzer, John E. Blume
  • Methods of analysis of alternative splicing in mouse
    Patent number: 7341835
    Abstract: The invention provides nucleic acid sequences which are complementary, in one embodiment, to a wide variety of mouse genes. The invention provides the sequences in such a way as to make them available for a variety of analyses. In one embodiment the nucleic acid sequences provided are present as an array of probes that may be used to measure gene expression of different mature RNA isoforms from at least 5,000 alternatively spliced mouse genes. As such, the invention relates to diverse fields impacted by the nature of molecular interaction, including chemistry, biology, medicine, pharmacology and medical diagnostics.
    Type: Grant
    Filed: January 13, 2005
    Date of Patent: March 11, 2008
    Assignee: Affymetrix, Inc.
    Inventors: John E. Blume, Alan J. Williams
  • Methods and reagents for diagnosis and treatment of diabetes
    Patent number: 7294698
    Abstract: The invention relates to methods and reagents for diagnosing and treating diabetes.
    Type: Grant
    Filed: November 1, 2006
    Date of Patent: November 13, 2007
    Assignee: Metabolex, Inc.
    Inventors: Jeffrey D Johnson, John E. Blume, John F. Palma, Yun-Ping Zhou
  • Methods and reagents for diagnosis and treatment of diabetes
    Patent number: 7144985
    Abstract: The invention relates to methods of determining islet cell activity by detecting the level of Archipelin or a fragment thereof and comparing the level to a baseline level or range associated with a known islet cell activity. Such methods are useful in diagnosing and studying the development of diabetes.
    Type: Grant
    Filed: December 2, 2002
    Date of Patent: December 5, 2006
    Assignee: Metabolex, Inc.
    Inventors: Jeffrey D. Johnson, John E. Blume, John F. Palma, Yun-Ping Zhou
  • Isolated islet beta-cell two-pore domain potassium channel
    Patent number: 7053180
    Abstract: The present invention provides polynucleotides encoding potassium channels as well as methods of using the potassium channels to treat and diagnose diabetes and a predisposition for diabetes.
    Type: Grant
    Filed: June 10, 2003
    Date of Patent: May 30, 2006
    Assignee: Metabolex, Inc.
    Inventors: Jeffrey D. Johnson, John F. Palma, Anthony C. Schweitzer, John E. Blume
  • Methods and compositions for treating and diagnosing diabetes
    Publication number: 20040072216
    Abstract: The present invention provides polynucleotides encoding potassium channels as well as methods of using the potassium channels to treat and diagnose diabetes and a predisposition for diabetes.
    Type: Application
    Filed: June 10, 2003
    Publication date: April 15, 2004
    Applicant: METABOLEX, INC.
    Inventors: Jeffrey D. Johnson, John F. Palma, Anthony C. Schweitzer, John E. Blume
  • Nucleic acids encoding a G-protein coupled receptor involved in islet cell signaling
    Publication number: 20040002114
    Abstract: The invention provides isolated nucleic acid and amino acid sequences of human and murine islet cell G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, methods of screening for modulators of islet cell G-protein coupled receptors and methods of treating mammals with modulators of islet cell G-protein coupled receptor activity.
    Type: Application
    Filed: January 6, 2003
    Publication date: January 1, 2004
    Applicant: METABOLEX, INC.
    Inventors: Francine M. Gregoire, Jeffrey D. Johnson, John E. Blume
  • Methods and reagents for diagnosis and treatment of diabetes
    Publication number: 20030202976
    Abstract: The invention relates to methods and reagents for diagnosing and treating diabetes.
    Type: Application
    Filed: December 2, 2002
    Publication date: October 30, 2003
    Applicant: METABOLEX, INC.
    Inventors: Jeffrey D. Johnson, John E. Blume, John F. Palma, Yun-Ping Zhou