Abstract: The present invention relates generally to a novel human T-cell lymphotropic, or leukemia, virus type II (HTLV-II) isolate designated NRA. HTLV-IINRA was originally isolated from a patient with atypical hairy cell leukemia. Preliminary restriction analysis of this isolate demonstrated that it differs genetically from the prototypical HTLV-II isolate Mo. HTLV-IINRA proviral molecular clones were obtained and the entire nucleotide sequence of the virus ascertained. The claimed invention is particularly directed toward the gp46 and p21e envelope proteins encoded by the env gene. Methods and kits for the detection of HTLV-II antibodies employing these envelope proteins are also described.

Abstract: Compositions including lysates, DNA and amino acid sequences derived from HTLV-IINRA are provided. Assays for detecting HTLV infection that employ the disclosed HTLV-IINRA compositions are also provided. Kits for performing HTLV assays are further provided.

Abstract: The method of orienting plastic pipe fittings in predetermined positions by passing the fittings downwardly through an inclined, rotating tube having a relatively smooth-walled bore so that the frictional agitation of the gravitating articles permits them to settle into a predetermined position with the center of mass in the lowermost possible position. The rate of rotation and the angle of incline are adjusted for different articles, and tubes of three different sizes are provided for interchangeable use. Two pairs of supporting rollers on opposite sides of the tube position are engageable with axially spaced annular collars on the tubes, the collars being sized to hold the tubes at the same discharge level and one of the rollers of each pair being driven by a variable speed motor to rotate the tube.

Abstract: An immunoassay which is capable of simultaneously detecting any desired number of antigens of one infectious agent, or combinations of antigens of several infectious agents, or any desired number of immunoglobulins of one infectious agent, or combinations of immunoglobulins of several infectious agents on a single solid support. A test sample is contacted with a solid support on which one or more antigens are immobilized as discrete test sites. Antigen-antibody complexes are formed and detected on the solid support.