Abstract: In the field of staining methods for histology and cytology specimens, the improvements of the invention are three-fold. First included is a drying-clearing step whereby a specimen is cleared by drying. Thus, the use of hazardous chemical-clearing agents to remove washing-dehydrating solution prior to cover-slipping is eliminated. Second, in a staining method using spray staining equipment, the use of a low molarity, combination, bluing-washing solution which serves the dual purpose of a washing solution and a bluing reagent, eliminates the use of water in a staining procedure. Third, and also in a staining method using spray staining equipment, the use of a series of sprays of fresh, essentially anhydrous, washing-dehydrating solution to dehydrate a specimen replaces immersion in a series of washing-dehydrating solutions and thereby decreases the amount of chemical consumed and also avoids contamination of the reagent with water and specimen debris.

Abstract: A plurality of glass slides having specimens such as blood smears disposed thereon are prepared for microscopic viewing by an apparatus which automatically applies preparation solutions to the slides such as stains and buffers and thereafter automatically drys the completed slides. The staining and buffering solutions are deposited evenly over the surfaces of the slides carrying the specimens by a timed operation of one or more electro-mechanically actuated aerosol dispensers which produce fine sprays of the desired preparation solutions. Simultaneously with the generated spray, the plurality of slides are rotated at a relatively slow rate to cause a uniform distribution of the spray particles on the slide surfaces. Following the staining and buffering mode, an automatic drying mode is initiated in which the plurality of slides are rotated at a relatively higher speed for a timed interval after which the apparatus automatically turns off and the completed slides may be removed from the apparatus.

Abstract: A plurality of glass slides having specimens such as blood smears disposed thereon are prepared for microscopic viewing by an apparatus which automatically applies preparation solutions to the slides such as stains and buffers and thereafter automatically drys the completed slides. The staining and buffering solutions are deposited evenly over the surfaces of the slides carrying the specimens by a timed operation of one or more electromechanically actuated aerosol dispensers which produce fine sprays of the desired preparation solutions. Simultaneously with the generated spray, the plurality of slides are rotated at a relatively slow rate to cause a uniform distribution of the spray particles on the slide surfaces. Following the staining and buffering mode, an automatic drying mode is initiated in which the plurality of slides are rotated at a relatively higher speed for a timed interval after which the apparatus automatically turns off and the completed slides may be removed from the apparatus.