Abstract: A purified enzyme mixture useful for isolating cells or cell clusters from tissue is disclosed. The mixture includes at least two collagenase enzymes, at least two other proteases, and additional non-protease components. The mixture is purified by removing at least some of the non-protease components. The purified mixture may then be used to isolate cells or cell clusters from tissue. Also disclosed are the essential components of the purified enzyme mixture as well as preferred ranges and ratios of these essential components for isolating cells or cell clusters from tissue. Finally, a dissociation system is disclosed that can be used with the purified enzyme mixture to dissociate tissue and recover cells or cell clusters.

Abstract: A purified enzyme mixture useful for isolating cells or cell clusters from tissue is disclosed. The mixture includes at least two collagenase enzymes, at least two other proteases, and additional non-protease components. The mixture is purified by removing at least some of the non-protease components. The purified mixture may then be used to isolate cells or cell clusters from tissue. Also disclosed are the essential components of the purified enzyme mixture as well as preferred ranges and ratios of these essential components for isolating cells or cell clusters from tissue. Finally, a dissociation system is disclosed that can be used with the purified enzyme mixture to dissociate tissue and recover cells or cell clusters.

Abstract: A suspension unit for roll-out shelves, drawers, etc. is provided for furniture cabinets and the like of the type having a case with slotted interior uprights. The suspension unit includes an inner housing member connected with a drawer or other roll-out furniture member, and an outer housing member operably connected with the inner housing member for smooth longitudinal sliding translation between the same. The outer housing member has prongs which engage the slotted upright when shifted longitudinally in a first direction. A resiliently flexible lock arm is connected with the outer housing member, and is configured to assume a normally outwardly protruding position. The lock arm is laterally flexible into an inwardly retracted position, and is oriented generally opposite the direction of the prongs, such that when the prongs are inserted into the slotted uprights, the lock arm abuts and adjacent upright to shift the lock arm into the retracted position.

Abstract: A tissue plasminogen activator (t-PA) variant is prepared that has an amino acid deletion at positions 466 to 470 of the corresponding wild-type t-PA. This alteration renders the variant fibrin (and plasma clot) specific as compared to the corresponding wild-type t-PA. DNA sequences can be prepared that encode the variant, as well as expression vectors incorporating the DNA sequences, and host cells transformed with the expression vectors. The variant may be used in a pharmaceutical preparation to treat a vascular disease or condition or to prevent fibrin deposition or adhesion formation or reformation in mammals.

Abstract: A tissue plasminogen activator (t-PA) variant is prepared that has an amino acid deletion at positions 466 to 470 of the corresponding wild-type t-PA. This alteration renders the variant fibrin (and plasma clot) specific as compared to the corresponding wild-type t-PA. DNA sequences can be prepared that encode the variant, as well as expression vectors incorporating the DNA sequences, and host cells transformed with the expression vectors. The variant may be used in a pharmaceutical preparation to treat a vascular disease or condition or to prevent fibrin deposition or adhesin formation or reformation in mammals.

Abstract: A wire stripping tool with two facing jaw surfaces that slide over one another about a fixed pivot axis in a shearing motion. Arcuate grooves are formed in the facing surfaces at equal radii from the axis. The grooves lead inwardly from substantially radial leading edges. Wire fed between the leading edges is initially engaged by the leading edges and the insulative wire cover is cut through. Simultaneously, the wire is shifted in orientation from a position substantially perpendicular to the axis. One jaw member then operates against the body of the wire insulation while the opposite member slides the cut insulation axially off the wire core.