Abstract: An improved method of culturing cells for cell therapy applications that includes growing desired cells in the presence of antigen-presenting cells and/or feeder cells and with medium volume to surface area ratio of up to 1 ml/cm2 if the growth surface is not comprised of gas permeable material and up to 2 ml/cm2 if the growth surface is comprised of gas permeable material. The desired cells are at a surface density of less than 0.5×106 cells/cm2 at the onset of a production cycle, and the surface density of the desired cells plus the surface density of the antigen presenting cells and/or feeder cells are at least about 1.25×105 cells/cm2.

Abstract: A device to which skis can be selectively attached for easily transporting the skis from one location to another when the skis are not in use, as well as facilitating storage and preventing theft of the skis while they are being stored. The device also preferably imprints snow for identification or other purposes when employed to transport the skis over snow.

Abstract: The disclosed invention is an apparatus for analyzing the annulus effluent of a producing well. The apparatus is designed to measure the flow rate of the annulus effluent, sample the annulus effluent and determine the quantities of each of the various phases of the annulus effluent. The apparatus is preferably mounted on a mobile vehicle and comprises a means for measuring the flow rate of the annulus effluent, a liquid vapor separator to split the annulus effluent into streams of entrained liquid and vapor, a separator to split the entrained liquid into the phases of oil and water, a sampling loop for extracting a sample of annulus effluent vapor which contains a condensor and a separator for splitting the fluid into its various phases, a means for measuring the amounts of all of the phases of the annulus effluent, and a means to record the data gathered. The apparatus is very useful in determining whether a producing well should be shut-in, worked over or left to produce "as is".

Abstract: The disclosed invention is a method for analyzing the annulus effluent of a producing well in a steam flood and using the information derived therefrom to determine whether to workover the production well for greater production, whether to allow the well to continue producing "as is", or whether to shut-in the annulus or the tubing production and approximately when the well should be worked over or shut-in.

Abstract: A method for collecting a non-pressurized sample of fluid from a well is disclosed and an illustrative sampler for performing the method is shown comprising two elongated sampler chamber interconnected upper and lower valves wherein the upper valve is mounted externally of the chamber and has a vent therein for providing less internal fluid drag in the chamber for improved and faster purging, filling, without subjecting the fluid to high shear rates, and emptying of the sample chamber and for ensuring a non-pressurized true representation sample upon arrival at the surface.