Abstract: This invention provides compositions and methods for rapid separation, isolation and purification of nucleic acids from biological samples using anionic exchange media. The method can utilize commercially available strong or weak anion exchanger materials with selected solutions of known ionic strength for adsorption and elution. The instant method is particularly advantageous as it permits the purification and identification of shorter fragments of nucleic acids from bodily fluids which, until now, had not been identified.

Abstract: This invention provides compositions and methods for rapid isolation and purification of nucleopolymers from biological samples using anionic exchange media. The method of this invention can utilize commercially available anion exchanger materials with selected solutions of known ionic strength for absorption and elution. The medium/nucleoprotein bound complex may be optionally stored or transported, and is subsequently treated with eluents to remove undesirable proteins and inorganic salts. The partially purified complex may also be stored or transported. This method is particularly advantageous since it allows rapid isolation and purification of soluble nucleic acids from a large volume of biological fluids with easy handling and storage, stabilizing the samples against degradation prior to analysis. It also permits the purification and identification of shorter fragments of nucleic acids from bodily fluids which, until now, had not been identified.

Abstract: This invention provides compositions and methods for rapid separation, isolation and purification of nucleic acids from biological samples using anionic exchange media. The method can utilize commercially available strong or weak anion exchanger materials with selected solutions of known ionic strength for adsorption and elution. The instant method is particularly advantageous as it permits the purification and identification of shorter fragments of nucleic acids from bodily fluids which, until now, had not been identified.

Abstract: Disclosed is a method and system for detecting a zombie attack in a network having a plurality of computers. The method and system include a network analysis module for determining, for each computer, a working set of email addresses associated with emails sent by each computer. A zombie attack is detected by determining at least one of: 1) at least one computer in the plurality is transmitting more than a threshold rate of emails, 2) that at least one of the computers is transmitting more than a first threshold number of emails to email addresses outside of its associated working set, 3) that a first threshold number of computers in the plurality are transmitting email messages to email addresses outside of their associated working set, and 4) that more than a second threshold number of computers are transmitting more than a second threshold number of emails to a recipient computer.

Abstract: This invention provides compositions and methods for rapid separation, isolation and purification of nucleopolymers, especially nucleic acids, from biological samples using anionic exchange media. The method of the present invention can utilize commercially available strong or weak anion exchanger materials with selected solutions of known ionic strength for adsorption and elution. The medium/nucleoprotein bound complex may be optionally stored or transported, but is subsequently treated with appropriate eluents to remove any undesirable proteins, including destabilizing enzymes, and inorganic salts and the like. The partially purified complex may also be stored or transported prior to further processing.

Abstract: Disclosed is a method and system for detecting a zombie attack in a network having a plurality of computers. The method and system include a network analysis module for determining, for each computer, a working set of email addresses associated with emails sent by each computer. A zombie attack is detected by determining at least one of: 1) at least one computer in the plurality is transmitting more than a threshold rate of emails, 2) that at least one of the computers is transmitting more than a first threshold number of emails to email addresses outside of its associated working set, 3) that a first threshold number of computers in the plurality are transmitting email messages to email addresses outside of their associated working set, and 4) that more than a second threshold number of computers are transmitting more than a second threshold number of emails to a recipient computer.

Abstract: Disclosed are compositions and methods for proximity-mediated rolling circle amplification and for real-time detection of proximity-mediated rolling circle amplification products. Rolling circle amplification (RCA) refers to nucleic acid amplification reactions involving replication of a circular nucleic acid template to form a long strand with tandem repeats of the sequence complementary to the circular template. In proximity-mediated RCA, binding guide conjugates are brought into close proximity, generally by associating them to the same analyte or to two analytes in close proximity. The binding guide conjugates comprise a specific binding molecule and a guide oligonucleotide. The guide oligonucleotides are complementary to guide complement portions on half circle probes.