Abstract: A system for inoculating and transporting Neisseria gonorrhoeae, or other microbial organisms enables inoculation and transport of a specimen suspected of containing Neisseria gonorrhoeae with an InTray® GC tray. The tray comprises a floor wall having a first depression and a second depression. A Gonococcal medium is disposed in the first depression. A removable inner panel overlays the first and second depressions. A removable outer panel overlays the inner panel. After introducing Neisseria gonorrhoeae or a microbial organism into the Gonococcal medium, the panels reseal the tray. Neisseria gonorrhoeae is introduced into the Gonococcal medium for inoculation thereof. A carbon dioxide member in the second depression releases a CO2 gas. A carbon dioxide seal covers the second depression. The seal is punctured to release the CO2 gas that saturates the medium in the first depression. The CO2 gas inhibits degradation of the Gonococcal medium or other microbes that require CO2.