Patents by Inventor John H. Leamon
John H. Leamon has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220356518Abstract: Methods and compositions for preparing nucleic acid libraries for nucleic acid sequencing are provided. In some embodiments, disclosed herein is a universal nucleic acid adaptor and methods of using same.Type: ApplicationFiled: April 29, 2022Publication date: November 10, 2022Applicant: Quantum-Si IncorporatedInventors: Jeffrey Sabina, Jonathan M. Rothberg, John H. Leamon
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Publication number: 20220120743Abstract: Described herein in an embodiment is a lateral flow assay strip for detecting the presence of one or more target nucleic acid sequences (e.g., one or more nucleic acid sequences of a pathogen, such as a coronavirus or an influenza virus). The lateral flow assay strip may comprise a first test line configured to detect a first target nucleic acid sequence and a flow control line configured to detect a liquid. In some cases, the first test line comprises a biological capture reagent (e.g., an immobilized antibody). In some cases, the flow control line comprises a non-biological fluid indicator (i.e., a non-antibody reagent). The non-biological fluid indicator may, for example, comprise a pH-sensitive material, a moisture-sensitive material, and/or a chemically-sensitive material. The flow control line may provide a visual indication of whether a fluidic sample was successfully transported from a first end of the lateral flow assay strip to the first test line.Type: ApplicationFiled: October 15, 2021Publication date: April 21, 2022Applicant: Detect, Inc.Inventors: Amy Cornell, Todd Roswech, John H. Leamon
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Publication number: 20220113307Abstract: Aspects of the disclosure relate to compositions and methods for amplifying and/or detecting one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens), and use of a trap region or reagent to reduce the level of one or more target nucleic acid analytes in an amplified sample prior to or in conjunction with detection. In some embodiments, the pathogens are viral, bacterial, fungal, parasitic, or protozoan pathogens, such as SARS-CoV-2 or an influenza virus. In some embodiments, the methods comprise isothermal amplification of a target nucleic acid and subsequent reduction in the level of one or more target nucleic acid analytes and detection of the amplification products.Type: ApplicationFiled: October 8, 2021Publication date: April 14, 2022Applicant: Detect, Inc.Inventors: Amy Cornell, John H. Leamon, Jonathan M. Rothberg, Henry Kemble, James A. Ball
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Publication number: 20210379591Abstract: Methods and devices for preparing target molecules (e.g., target nucleic acids or target proteins) from a biological sample are provided herein. In some embodiments, methods and devices involve sample lysis, sample fragmentation, enrichment of target molecule(s), and/or functionalization of target molecule(s).Type: ApplicationFiled: April 21, 2021Publication date: December 9, 2021Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, John H. Leamon, Jonathan C. Schultz, Michele Millham, Caixia Lv, Haidong Huang, Roger Nani, Omer Ad, Brian Reed, Matthew Dyer, Robert E. Boer
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Publication number: 20210354133Abstract: Methods and devices for preparing target molecules (e.g., target nucleic acids or target proteins) from a biological sample are provided herein. In some embodiments, methods and devices involve sample lysis, sample fragmentation, enrichment of target molecule(s), and/or functionalization of target molecule(s).Type: ApplicationFiled: April 21, 2021Publication date: November 18, 2021Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, John H. Leamon, Jonathan C. Schultz, Michele Millham, Caixia Lv, Haidong Huang, Roger Nani, Omer Ad, Brian Reed, Matthew Dyer, Robert E. Boer
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Publication number: 20210354134Abstract: Methods and devices for preparing target molecules (e.g., target nucleic acids or target proteins) from a biological sample are provided herein. In some embodiments, methods and devices involve sample lysis, sample fragmentation, enrichment of target molecule(s), and/or functionalization of target molecule(s).Type: ApplicationFiled: April 21, 2021Publication date: November 18, 2021Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, John H. Leamon, Jonathan C. Schultz, Michele Millham, Caixia Lv, Haidong Huang, Roger Nani, Omer Ad, Brian Reed, Matthew Dyer, Robert E. Boer
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Publication number: 20210331170Abstract: Methods and devices for preparing target molecules (e.g., target nucleic acids or target proteins) from a biological sample are provided herein. In some embodiments, methods and devices involve sample lysis, sample fragmentation, enrichment of target molecule(s), and/or functionalization of target molecule(s).Type: ApplicationFiled: April 21, 2021Publication date: October 28, 2021Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, John H. Leamon, Jonathan C. Schultz, Michele Millham, Caixia Lv, Haidong Huang, Roger Nani, Omer Ad, Brian Reed, Matthew Dyer, Robert E. Boer
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Publication number: 20210291176Abstract: Provided herein, in some embodiments, are rapid diagnostic tests to detect one or more target nucleic acid sequences (e.g., a nucleic acid sequence of one or more pathogens). In some embodiments, the pathogens are viral, bacterial, fungal, parasitic, or protozoan pathogens, such as SARS-CoV-2 or an influenza virus. Further embodiments provide methods of detecting genetic abnormalities. Diagnostic tests comprising a sample-collecting component, one or more reagents (e.g., lysis reagents, nucleic acid amplification reagents), and a detection component (e.g., a component comprising a lateral flow assay strip) are provided.Type: ApplicationFiled: March 16, 2021Publication date: September 23, 2021Applicant: Detect, Inc.Inventors: Todd Roswech, John H. Leamon, Jonathan M. Rothberg
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Publication number: 20210291177Abstract: A reagent carrier includes: a cap configured to fit on an opening of a reaction vessel; and a reagent confined by the cap. The cap may be configured to release the reagent when a user manipulates the cap while the cap is fitted on the opening of the reaction vessel. For example, the reagent may be released when the user twists the cap from a first position to a second position or when the user pushes on a surface of the cap. The cap may be configured to seal the opening of the reaction vessel when the cap is fitted on the opening of the reaction vessel. In some implementations, the cap may be a blister cap containing the reagent. In some implementations, the cap may include a cage containing the reagent. In some implementations, the cap may include a deformable structure containing the reagent.Type: ApplicationFiled: March 16, 2021Publication date: September 23, 2021Applicant: Detect, Inc.Inventors: Jonathan M. Rothberg, Spencer Glantz, Benjamin Rosenbluth, Todd Roswech, Eric Kauderer-Abrams, Matthew Dyer, Jose Camara, Owen Kaye-Kauderer, John H. Leamon
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Publication number: 20210164035Abstract: Methods and devices for enriching and analyzing target molecules from a sample are provided herein. In some embodiments, methods and devices involve enrichment of target molecules (e.g., using SCODA) and subsequent detection and analysis using sequencing.Type: ApplicationFiled: October 28, 2020Publication date: June 3, 2021Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, John H. Leamon, Jonathan C. Schultz, Michele Millham, Caixia Lv, Xiaxiao Ma
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Publication number: 20210121874Abstract: Embodiments described herein generally relate to apparatuses, cartridges, and pumps for peristaltic pumping of fluids and associated methods, systems, and devices. The pumping of fluids is, in certain cases, an important aspect of a variety of applications, such as bioanalytical applications (e.g., biological sample analysis, sequencing, identification). The inventive features described herein may, in some embodiments, provide an ability to pump fluids in ways that combine certain advantages of robotic fluid handling systems (e.g., automation, programmability, configurability, flexibility) with certain advantages of microfluidics (e.g., small fluid volumes with high fluid resolution, precision, monolithic consumables, limiting of the wetting of components to consumables).Type: ApplicationFiled: October 28, 2020Publication date: April 29, 2021Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, Jonathan C. Schultz, John H. Leamon, Todd Roswech, Xiaxiao Ma
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Publication number: 20210121875Abstract: Embodiments described herein generally relate to apparatuses, cartridges, and pumps for peristaltic pumping of fluids and associated methods, systems, and devices. The pumping of fluids is, in certain cases, an important aspect of a variety of applications, such as bioanalytical applications (e.g., biological sample analysis, sequencing, identification). The inventive features described herein may, in some embodiments, provide an ability to pump fluids in ways that combine certain advantages of robotic fluid handling systems (e.g., automation, programmability, configurability, flexibility) with certain advantages of microfluidics (e.g., small fluid volumes with high fluid resolution, precision, monolithic consumables, limiting of the wetting of components to consumables).Type: ApplicationFiled: October 28, 2020Publication date: April 29, 2021Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, Jonathan C. Schultz, John H. Leamon, Todd Roswech, Xiaxiao Ma
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Publication number: 20210121879Abstract: Methods and devices for isolating or enriching target molecules from a sample are provided herein. In some embodiments, methods and devices further involve detection, analysis and/or sequencing of a target molecule.Type: ApplicationFiled: October 28, 2020Publication date: April 29, 2021Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, John H. Leamon, Jonathan C. Schultz, Michele Millham, Caixia Lv, Xiaxiao Ma
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Patent number: 10982274Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.Type: GrantFiled: January 16, 2019Date of Patent: April 20, 2021Assignee: Roche Molecular Systems, Inc.Inventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steven Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
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Publication number: 20200123594Abstract: Methods and devices for enriching and analyzing target molecules from a sample are provided herein. In some embodiments, methods and devices involve enrichment of target molecules (e.g., using SCODA) and subsequent detection and analysis using sequencing.Type: ApplicationFiled: October 29, 2019Publication date: April 23, 2020Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, John H. Leamon, Jonathan C. Schultz, Michele Millham, Cassie Lv, Xiaxiao Ma
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Publication number: 20200123593Abstract: Methods and devices for isolating or enriching target molecules from a sample are provided herein. In some embodiments, methods and devices further involve detection, analysis and/or sequencing of a target molecule.Type: ApplicationFiled: October 29, 2019Publication date: April 23, 2020Applicant: Quantum-Si IncorporatedInventors: Jonathan M. Rothberg, John H. Leamon, Jonathan C. Schultz, Michele Millham, Cassie Lv, Xiaxiao Ma
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Publication number: 20190203263Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.Type: ApplicationFiled: January 16, 2019Publication date: July 4, 2019Inventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steve Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
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Patent number: 10240192Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.Type: GrantFiled: June 23, 2016Date of Patent: March 26, 2019Assignee: 454 Life Sciences CorporationInventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steven Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
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Patent number: 10209250Abstract: A method for performing a combined protein and nucleic acid assay on a target captured by a capture agent, includes providing a microfluidic device having a microfluidic channel network having at least one microfluidic channel, the channel arranged to receive fluid, the device having at least two micro-particles disposed in fixed position in the channel, the micro-particles being functionalized with a capture agent for the assay, one of the micro-particles in the channel being functionalized with an antibody or antigen capture agent and another of the micro-particles being functionalized with a nucleic acid capture agent.Type: GrantFiled: June 30, 2017Date of Patent: February 19, 2019Assignee: CyVek, Inc.Inventors: Martin A. Putnam, John H. Leamon, Jeffrey T. Branciforte, Charles O. Stanwood
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Publication number: 20170307601Abstract: A method for performing a combined protein and nucleic acid assay on a target captured by a capture agent, includes providing a microfluidic device having a microfluidic channel network having at least one microfluidic channel, the channel arranged to receive fluid, the device having at least two micro-particles disposed in fixed position in the channel, the micro-particles being functionalized with a capture agent for the assay, one of the micro-particles in the channel being functionalized with an antibody or antigen capture agent and another of the micro-particles being functionalized with a nucleic acid capture agent.Type: ApplicationFiled: June 30, 2017Publication date: October 26, 2017Inventors: Martin A. Putnam, John H. Leamon, Jeffrey T. Branciforte, Charles O. Stanwood