Patents by Inventor John H. Leamon

John H. Leamon has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20190203263
    Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
    Type: Application
    Filed: January 16, 2019
    Publication date: July 4, 2019
    Inventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steve Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
  • Patent number: 10240192
    Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
    Type: Grant
    Filed: June 23, 2016
    Date of Patent: March 26, 2019
    Assignee: 454 Life Sciences Corporation
    Inventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steven Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
  • Patent number: 10209250
    Abstract: A method for performing a combined protein and nucleic acid assay on a target captured by a capture agent, includes providing a microfluidic device having a microfluidic channel network having at least one microfluidic channel, the channel arranged to receive fluid, the device having at least two micro-particles disposed in fixed position in the channel, the micro-particles being functionalized with a capture agent for the assay, one of the micro-particles in the channel being functionalized with an antibody or antigen capture agent and another of the micro-particles being functionalized with a nucleic acid capture agent.
    Type: Grant
    Filed: June 30, 2017
    Date of Patent: February 19, 2019
    Assignee: CyVek, Inc.
    Inventors: Martin A. Putnam, John H. Leamon, Jeffrey T. Branciforte, Charles O. Stanwood
  • Publication number: 20170307601
    Abstract: A method for performing a combined protein and nucleic acid assay on a target captured by a capture agent, includes providing a microfluidic device having a microfluidic channel network having at least one microfluidic channel, the channel arranged to receive fluid, the device having at least two micro-particles disposed in fixed position in the channel, the micro-particles being functionalized with a capture agent for the assay, one of the micro-particles in the channel being functionalized with an antibody or antigen capture agent and another of the micro-particles being functionalized with a nucleic acid capture agent.
    Type: Application
    Filed: June 30, 2017
    Publication date: October 26, 2017
    Inventors: Martin A. Putnam, John H. Leamon, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 9759718
    Abstract: A microfluidic device comprising a microfluidic channel network sealed on one side by a membrane sheet, the sheet having PDMS defining at least the surface sealing the channel, the membrane sheet on its opposite side sealing one side of a pneumatic channel, the pneumatic channel arranged to enable pneumatic deflection of a deflectable portion of the membrane sheet into contact with an opposed surface to control flow in a channel of the network, the membrane sheet confining in a channel of the network at least one micro-particle, micro-length tube or glass nano reactor, functionalized with a capture agent, that has been inserted into that channel. A microfluidic device having a microfluidic channel containing at least two micro-particles, micro-length tubes or glass nano reactors, one functionalized with nucleic acid and another with antibody or antigen.
    Type: Grant
    Filed: September 6, 2014
    Date of Patent: September 12, 2017
    Assignee: CyVek, Inc.
    Inventors: Martin A. Putnam, John H. Leamon, Jeffrey T. Branciforte, Charles O. Stanwood
  • Publication number: 20160298175
    Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
    Type: Application
    Filed: June 23, 2016
    Publication date: October 13, 2016
    Inventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steven Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
  • Publication number: 20150099672
    Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
    Type: Application
    Filed: October 20, 2014
    Publication date: April 9, 2015
    Inventors: JAN BERKA, YI-JU CHEN, JOHN H. LEAMON, STEVEN LEFKOWITZ, KENTON L. LOHMAN, VINOD B. MAKHIJANI, JONATHAN M. ROTHBERG, GARY J. SARKIS, MAITHREYAN SRINIVASAN, MICHAEL P. WEINER
  • Publication number: 20150087559
    Abstract: A microfluidic device comprising a microfluidic channel network sealed on one side by a membrane sheet, the sheet having PDMS defining at least the surface sealing the channel, the membrane sheet on its opposite side sealing one side of a pneumatic channel, the pneumatic channel arranged to enable pneumatic deflection of a deflectable portion of the membrane sheet into contact with an opposed surface to control flow in a channel of the network, the membrane sheet confining in a channel of the network at least one micro-particle, micro-length tube or glass nano reactor, functionalized with a capture agent, that has been inserted into that channel. A microfluidic device having a microfluidic channel containing at least two micro-particles, micro-length tubes or glass nano reactors, one functionalized with nucleic acid and another with antibody or antigen.
    Type: Application
    Filed: September 6, 2014
    Publication date: March 26, 2015
    Inventors: Martin A. Putnam, John H. Leamon, Jeffrey T. Branciforte, Charles O. Stanwood
  • Patent number: 8906617
    Abstract: The invention provides apparatuses and methods of use thereof for sequencing nucleic acids subjected to a force, and thus considered under tension. The methods may employ but are not dependent upon incorporation of extrinsically detectably labeled nucleotides.
    Type: Grant
    Filed: March 9, 2011
    Date of Patent: December 9, 2014
    Assignee: Life Technologies Corporation
    Inventors: Jonathan M. Rothberg, John H. Leamon, John F. Davidson, Antoine M. Van Oijen, Wolfgang Hinz, Melville Davey, Bradley Hann, Jonathan Schultz
  • Patent number: 8790876
    Abstract: An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
    Type: Grant
    Filed: April 20, 2007
    Date of Patent: July 29, 2014
    Assignee: 454 Life Sciences Corporation
    Inventors: John H. Leamon, Kenton L. Lohman, Jonathan M. Rothberg, Michael P. Weiner
  • Patent number: 8765380
    Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
    Type: Grant
    Filed: September 14, 2012
    Date of Patent: July 1, 2014
    Assignee: 454 Life Sciences Corporation
    Inventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steve Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyn Srinivasan, Michael P. Weiner
  • Publication number: 20140162885
    Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
    Type: Application
    Filed: September 25, 2013
    Publication date: June 12, 2014
    Applicant: 454 LIFE SCIENCES CORPORATION
    Inventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steven Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
  • Patent number: 8748102
    Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the beads. Also disclosed are kits and apparatuses for performing the methods of the invention.
    Type: Grant
    Filed: February 23, 2011
    Date of Patent: June 10, 2014
    Assignee: 454 Life Sciences Corporation
    Inventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steve Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
  • Patent number: 8524057
    Abstract: Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates.
    Type: Grant
    Filed: July 28, 2011
    Date of Patent: September 3, 2013
    Assignee: Life Technologies Corporation
    Inventors: Jonathan M. Rothberg, Wolfgang Hinz, Kim L. Johnson, James M. Bustillo, John H. Leamon, Jonathan Schultz
  • Publication number: 20130217004
    Abstract: Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
    Type: Application
    Filed: September 12, 2012
    Publication date: August 22, 2013
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jonathan M. ROTHBERG, Wolfgang Hinz, John F. Davidson, Antoine M. van Oijen, John H. Leamon, Martin Huber
  • Publication number: 20130078638
    Abstract: Disclosed are methods for nucleic acid amplification wherein nucleic acid templates, beads, and amplification reaction solution are emulsified and the nucleic acid templates are amplified to provide clonal copies of the nucleic acid templates attached to the heads. Also disclosed are kits and apparatuses for performing the methods of the invention.
    Type: Application
    Filed: September 14, 2012
    Publication date: March 28, 2013
    Inventors: Jan Berka, Yi-Ju Chen, John H. Leamon, Steve Lefkowitz, Kenton L. Lohman, Vinod B. Makhijani, Jonathan M. Rothberg, Gary J. Sarkis, Maithreyan Srinivasan, Michael P. Weiner
  • Publication number: 20120238475
    Abstract: An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
    Type: Application
    Filed: March 13, 2012
    Publication date: September 20, 2012
    Inventors: John H. Leamon, Kenton L. Lohman, Jonathan M. Rothberg, Michael P. Weiner
  • Patent number: 8158359
    Abstract: An apparatus and method for performing rapid DNA sequencing, such as genomic sequencing, is provided herein. The method includes the steps of preparing a sample DNA for genomic sequencing, amplifying the prepared DNA in a representative manner, and performing multiple sequencing reaction on the amplified DNA with only one primer hybridization step.
    Type: Grant
    Filed: June 24, 2010
    Date of Patent: April 17, 2012
    Assignee: 454 Lice Sciences Corporation
    Inventors: John H. Leamon, Kenton L. Lohman, Jonathan M. Rothberg, Michael P. Weiner
  • Publication number: 20110281741
    Abstract: Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
    Type: Application
    Filed: February 15, 2011
    Publication date: November 17, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jonathan M. ROTHBERG, Wolfgang HINZ, John F. DAVIDSON, Antoine M. van OIJEN, John H. LEAMON, Martin HUBER
  • Publication number: 20110281737
    Abstract: Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions.
    Type: Application
    Filed: February 15, 2011
    Publication date: November 17, 2011
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Jonathan M. ROTHBERG, Wolfgang Hinz, John F. Davidson, Antoine M. van Oijen, John H. Leamon, Martin Huber