Abstract: A process for producing chitin nanocrystals (ChNCs) involves contacting a chitinous material with a sufficient amount of an inorganic persulfate to produce chitin nanocrystals from the chitinous material. The process permits one-spot production of ChNCs from biomasses such as crustaceans, fungi, mushrooms, insects or mixtures thereof. Chitin nanocrystals produced by the process comprise surface carboxylic groups and are not initially deacetylated.

Abstract: A process for producing chitin nanocrystals (ChNCs) involves contacting a chitinous material with a sufficient amount of an inorganic persulfate to produce chitin nanocrystals from the chitinous material. The process permits one-spot production of ChNCs from biomasses such as crustaceans, fungi, mushrooms, insects or mixtures thereof. Chitin nanocrystals produced by the process comprise surface carboxylic groups and are not initially deacetylated.

Abstract: A water soluble ligand bound polymer has been synthesized and used for purification and stabilization of trypsin, an easily autodigestible enzyme. The affinity polymer was formed by copolymerizing N-acryloyl-m-amino-benzamidine, and acrylamide in the absence of oxygen. Bound trypsin could be easily eluted by either arginine or benzamidine. At low temperature (<5.degree. C.), the polymer solution was very stable and retained its high capacity for trypsin binding after 6 months of storage. Trypsin can also be stored in this system for extended periods. Combining the principles of affinity chromatography and ultrafiltration, a process has been developed, using this polymer, for purification of trypsin. The purification process also features provisions for the recirculation of the eluant as well as the macroligand.