Abstract: The invention relates to methods for identifying polypeptides and polynucleotides of interest, be they novel or variant polypeptides and polynucleotides, by expressing a plurality of polypeptides in an obligate or facultative anaerobe that is incapable of, or displays a reduction in, the oxidation of NADH and/or NADPH under anaerobic fermentation conditions and selecting an obligate or facultative anaerobe that grows or displays a growth advantage under said conditions. The invention is also concerned with novel enzymes per se, and their use in enzymatic production processes.

Abstract: A holding tank cover includes a gas permeable insulating membrane and a gas impermeable cover layer sized to overlay and conceal the gas permeable membrane. Together, the gas permeable membrane and the gas impermeable cover layer facilitate a controlled transmission of gas between an interior of the holding tank and an environment external to the holding tank.

Abstract: A holding tank cover includes a gas permeable insulating membrane and a gas impermeable cover layer sized to overlay and conceal the gas permeable membrane. Together, the gas permeable membrane and the gas impermeable cover layer facilitate a controlled transmission of gas between an interior of the holding tank and an environment external to the holding tank.

Abstract: A holding tank cover includes a gas permeable insulating membrane and a gas impermeable cover layer sized to overlay and conceal the gas permeable membrane. Together, the gas permeable membrane and the gas impermeable cover layer facilitate a controlled transmission of gas between an interior of the holding tank and an environment external to the holding tank.

Abstract: The present invention provides a bacterial expression system for expressing a nucleic acid comprising: (a) DNA encoding a group 5 RNA polymerase sigma factor; and (b) an expression cassette comprising a promoter recognised by the group 5 RNA polymerase sigma factor operably linked to a heterologous nucleic acid; wherein (a) and (b) are located on the same expression vector, separate expression vectors or are integrated into the bacterial host genome.

Abstract: A holding tank cover includes a gas permeable insulating membrane and a gas impermeable cover layer sized to overlay and conceal the gas permeable membrane. Together, the gas permeable membrane and the gas impermeable cover layer facilitate a controlled transmission of gas between an interior of the holding tank and an environment external to the holding tank.

Abstract: A boat hull cleaner having a handle (not shown) and a knuckle (see FIG. 1), the knuckle 1 having a first portion 2 and a second portion 3, the first portion 2 having a first channel and the second portion 3 having a second channel, the first portion 2 having a first connector part 4 to be joined to a first tubular part of the handle, and the second portion 3 having a second connector part 5 joined to a second tubular part of the handle; the arrangement being such that a fluid can be caused to flow within the first tubular part of the handle, into the first and second channels of the knuckle 1, and then into the second tubular part of the handle, and wherein the first and second portions 2 and 3 of the knuckle 1 can be adjusted with respect to one another such that the first and second tubular parts of the handle assume an angle with respect to one another.