Publication number: 20070092861
Abstract: Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, an ECM protein, or a biologically active fragment or variant thereof such as elastin, fibronectin, vitronectin, lamninin, collagen I, collagen III, collagen IV, and collagen VI. Also, optionally present on the surface is an active factor, preferably a polycationic polymer or a biologically active fragment or variant thereof, such as polyethyleneimine (PEI), poly-D-lysine (PDL), poly-L-lysine (PLL), poly-D-ornithine (PDO) or poly-L-ornithine (PLO). This surface is used in cell culture to promote cell attachment, survival, maintenance of function, and/or proliferation of primary liver cells. The invention also relates to methods utilizing this surface, such as methods for attachment, survival, maintenance of function, and/or proliferation of cells. Further disclosed is the use of the surface in cell culture with serum-free medium.
Type:
Application
Filed:
September 10, 2004
Publication date:
April 26, 2007
Inventors:
Richard Guarino, Sharon Presnell, Andrea Liebmann-Vinson, Mohammed Heidaran, John Hemperly, Stewart Jurgensen, Ronald Laethem, Neil Robbins
Publication number: 20050058687
Abstract: Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and/or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.
Type:
Application
Filed:
September 12, 2003
Publication date:
March 17, 2005
Applicant:
Becton, Dickinson and Company
Inventors:
Richard Guarino, Jonathan Rowley, Andrea Liebmann-Vinson, John Hemperly, Mohammad Heidaran, Sharon Presnell