Patents by Inventor John HUGHES, JR.
John HUGHES, JR. has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11744251Abstract: A mixing apparatus may include a bowl defining at least one bowl opening for contents of the bowl to exit therefrom, and a valve assembly configured to selectively attach to the bowl at the bowl opening and including at least one base portion defining a base opening and configured to selectively receive a seal portion, the seal portion being movable between open and close positions, wherein in the open position, the base opening is unobstructed allowing contents of the bowl exit the bowl via the base opening, and in the closed position, the seal portion blocks the base opening preventing contents from exiting the bowl.Type: GrantFiled: April 16, 2020Date of Patent: September 5, 2023Inventor: John Hughes, Jr.
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Publication number: 20200329718Abstract: A mixing apparatus may include a bowl defining at least one bowl opening for contents of the bowl to exit therefrom, and a valve assembly configured to selectively attach to the bowl at the bowl opening and including at least one base portion defining a base opening and configured to selectively receive a seal portion, the seal portion being movable between open and close positions, wherein in the open position, the base opening is unobstructed allowing contents of the bowl exit the bowl via the base opening, and in the closed position, the seal portion blocks the base opening preventing contents from exiting the bowl.Type: ApplicationFiled: April 16, 2020Publication date: October 22, 2020Inventor: John HUGHES, JR.
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Patent number: 8518670Abstract: The present invention provides thermostable enzymes, such as DNA polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (PCR).Type: GrantFiled: July 27, 2011Date of Patent: August 27, 2013Assignee: Life Technologies CorporationInventors: Adam Goldstein, A. John Hughes, Jr.
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Publication number: 20110306112Abstract: The present invention provides substantially pure reverse transcriptases, which are preferably substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in synthesizing, amplifying or sequencing nucleic acid molecules, including through the use of the polymerase chain reaction, particularly RT-PCR.Type: ApplicationFiled: May 13, 2011Publication date: December 15, 2011Applicant: LIFE TECHNOLOGIES, INC.Inventor: A. John Hughes, JR.
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Patent number: 8062848Abstract: The present invention provides thermostable enzymes, such as DNA polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (PCR).Type: GrantFiled: April 30, 2009Date of Patent: November 22, 2011Assignee: Life Technologies CorporationInventors: Adam Goldstein, A. John Hughes, Jr.
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Publication number: 20110281276Abstract: The present invention provides thermostable enzymes, such as DNA polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (PCR).Type: ApplicationFiled: July 27, 2011Publication date: November 17, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Adam S. Goldstein, A. John Hughes, JR.
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Publication number: 20090215126Abstract: The present invention provides thermostable enzymes, such as DNA polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (PCR).Type: ApplicationFiled: April 30, 2009Publication date: August 27, 2009Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Adam S. Goldstein, A. John Hughes, JR.
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Publication number: 20090155775Abstract: The invention relates to a substantially pure thermostable DNA polymerase from Thermotoga (Tne and Tma) and mutants thereof. The Tne DNA polymerase has a molecular weight of about 100 kilodaltons and is more thermostable than Taq DNA polymerase. The mutant DNA polymerase has at least one mutation selected from the group consisting of (1) a first mutation that substantially reduces or eliminates 3??5? exonuclease activity of said DNA polymerase; (2) a second mutation that substantially reduces or eliminates 5??3? exonuclease activity of said DNA polymerase; (3) a third mutation in the O helix of said DNA polymerase resulting in said DNA polymerase becoming non-discriminating against dideoxynucleotides. The present invention also relates to the cloning and expression of the wild type or mutant DNA polymerases in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes.Type: ApplicationFiled: June 8, 2007Publication date: June 18, 2009Applicant: INVITROGEN CORPORATIONInventors: Deb K. Chatterjee, A. John Hughes, JR.
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Publication number: 20090029436Abstract: The present invention provides substantially pure reverse transcriptases, which are preferably substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in synthesizing, amplifying or sequencing nucleic acid molecules, including through the use of the polymerase chain reaction, particularly RT-PCR.Type: ApplicationFiled: October 1, 2008Publication date: January 29, 2009Applicant: INVITROGEN CORPORATIONInventor: A. John Hughes, JR.
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Patent number: 6905858Abstract: The present invention provides thermostable enzymes, such as DNA polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (PCR).Type: GrantFiled: January 15, 2003Date of Patent: June 14, 2005Assignee: Invitrogen CorporationInventors: Adam S. Goldstein, A. John Hughes, Jr.
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Patent number: 6630333Abstract: The present invention provides substantially pure reverse transcriptases, which are preferably substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in synthesizing, amplifying or sequencing nucleic acid molecules including through the use of the polymerase chain reaction, particularly RT-PCR.Type: GrantFiled: March 23, 2000Date of Patent: October 7, 2003Assignee: Invitrogen CorporationInventor: A. John Hughes, Jr.
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Patent number: 6531301Abstract: The present invention provides thermostable enzymes, such as DNA polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (PCR).Type: GrantFiled: May 30, 2001Date of Patent: March 11, 2003Assignee: Invitrogen CorporationInventors: Adam S. Goldstein, A. John Hughes, Jr.
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Patent number: 6444424Abstract: The invention relates to a substantially pure thermostable DNA polymerase from Thermotoga neapolitana (Tne). The Tne DNA polymerase has a molecular weight of about 100 kilodaltons and is more thermostable than Taq DNA polymerase. The present invention also relates to the cloning and expression of the Tne DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes. The Tne DNA polymerase of the invention may be used in well-known DNA sequencing and amplification reactions.Type: GrantFiled: January 26, 1999Date of Patent: September 3, 2002Assignee: Invitrogen CorporationInventors: Deb K. Chatterjee, A. John Hughes, Jr.
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Patent number: 6245533Abstract: The present invention provides thermostable enzymes, such as DNA polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (PCR).Type: GrantFiled: January 14, 1999Date of Patent: June 12, 2001Assignee: Invitrogen CorporationInventors: Adam S. Goldstein, A. John Hughes, Jr.
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Patent number: 5939301Abstract: The invention relates to a substantially pure thermostable DNA polymerase from Thermotoga neapolitana (Tne) and mutants thereof. The Tne DNA polymerase has a molecular weight of about 100 kilodaltons and is more thermostable than Taq DNA polymerase. The mutant Tne DNA polymerase has at least one mutation selected from the group consisting of (1) a first mutation that substantially reduces or eliminates 3'.fwdarw.5' exonuclease activity of said DNA polymerase; (2) a second mutation that substantially reduces or eliminates 5'.fwdarw.3' exonuclease activity of said DNA polymerase; (3) a third mutation in the O helix of said DNA polymerase resulting in said DNA polymerase becoming non-discriminating against dideoxynucleotides. The present invention also relates to the cloning and expression of the wild type or mutant Tne DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes.Type: GrantFiled: October 2, 1995Date of Patent: August 17, 1999Assignee: Life Technologies, Inc.Inventors: A. John Hughes, Jr., Deb K. Chatterjee
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Patent number: 5912155Abstract: The invention relates to a substantially pure thermostable DNA polymerase from Thermotoga neapolitana (Tne). The Tne DNA polymerase has a molecular weight of about 100 kilodaltons and is more thermostable than Taq DNA polymerase. The present invention also relates to the cloning and expression of the Tne DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes. The Tne DNA polymerase of the invention may be used in well-known DNA sequencing and amplification reactions.Type: GrantFiled: January 9, 1995Date of Patent: June 15, 1999Assignee: Life Technologies, Inc.Inventors: Deb K. Chatterjee, A. John Hughes, Jr.
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Patent number: 5861295Abstract: The present invention provides thermostable enzymes, such as DNA polymerases and restriction endonucleases, that are substantially free from contamination with nucleic acids. The invention also provides methods for the production of these enzymes, and kits comprising these enzymes which may be used in amplifying or sequencing nucleic acid molecules, including through use of the polymerase chain reaction (PCR).Type: GrantFiled: January 2, 1997Date of Patent: January 19, 1999Assignee: Life Technologies, Inc.Inventors: Adam S. Goldstein, A. John Hughes, Jr.
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Patent number: H1531Abstract: The invention relates to a substantially pure thermostable DNA polymerase. Preferably, the DNA polymerase has a molecular weight of about 95 kilodaltons and is more thermostable than Taq DNA polymerase. The present invention also relates to cloning and expression of the DNA polymerase in E. coli, to DNA molecules containing the cloned gene, and to host cells which express said genes.Type: GrantFiled: April 18, 1994Date of Patent: May 7, 1996Inventors: Ilse I. Blumentals, Roger S. Lasken, Brian J. Schmidt, Mary C. Longo, A. John Hughes, Jr., Deb K. Chatterjee