Abstract: The present disclosure provide modified yeast that produce increased quantities of RNA bioactive molecules and methods of producing the same. Also provided are methods and uses of the yeast for biocontrol and disease protection.

Abstract: The present disclosure provides yeast transformed with a nucleic acid molecule (GAT1) to reduce nitrogen catabolite repression of asparagine transport/degradation and/or overexpress genes (ASP1 or ASP3) encoding cell-wall or extracellular proteins involved in asparagine degradation and/or genes (AGP1 or GNP1 or GAP1) encoding proteins involved in asparagine transport under food preparation/processing conditions. The genetically modified yeast has enhanced ability to reduce acnlamide concentration in foods prepared by heating. Also provided are methods and uses of the transgenic yeast for reducing acnlamide in a food product and food products having reduced acrylamide content prepared using the transgenic yeast.

Abstract: The present disclosure provides yeast transformed with a nucleic acid molecule (GAT1) to reduce nitrogen catabolite repression of asparagine transport/degradation and/or overexpress genes (ASP1 or ASP3) encoding cell-wall or extracellular proteins involved in asparagine degradation and/or genes (AGP1 or GNP1 or GAP1) encoding proteins involved in asparagine transport under food preparation/processing conditions. The genetically modified yeast has enhanced ability to reduce acnlamide concentration in foods prepared by heating. Also provided are methods and uses of the transgenic yeast for reducing acnlamide in a food product and food products having reduced acrylamide content prepared using the transgenic yeast.

Abstract: The invention provides a Saccharomyces cerevisiae strain that is transformed to constitutively express DUR3, encoding a urea transporter protein, under the control of the phosphoglycerate kinase (PGK1) promoter and terminator sequences, resulting in reduced nitrogen catabolite repression, wherein said transformed yeast strain may be further transformed to constitutively express a urea degradation enzyme, such as urea carboxylase-allophanate hydrolase or urea amidolyase, also resulting in reduced nitrogen catabolite repression, a method for generating said strain, and a method and use of said strain to produce a fermented beverage or food product with a reduced ethyl carbamate concentration of less than 30 ppb.