Patents by Inventor John J. Mekalanos

John J. Mekalanos has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 5599537
    Abstract: A vector capable of integrating into the chromosome of Salmonella including a first DNA sequence encoding a heterologous protein, a second DNA sequence encoding a marker, and a third DNA sequence encoding a phoP regulatory region regulated gene product necessary for virulence, the third DNA sequence being mutationally inactivated.
    Type: Grant
    Filed: July 9, 1993
    Date of Patent: February 4, 1997
    Assignees: The General Hospital Corporation, President and Fellows of Harvard College
    Inventors: Samuel I. Miller, III, John J. Mekalanos
  • Patent number: 5571688
    Abstract: A reporter system relating to in vivo expression technology was devised to aid in the identification and study of genes that display temporal or spatial patterns of expression during infection of host tissues. The method of this invention comprises constructing a strain or pool of strains of a microorganism which contains an artificial cointegrate comprising a reporter gene flanked by direct repeats of sequences to which a resolvase enzyme binds, thus catalyzing excision of the reporter gene, and further contains a coding sequence under the control of a promoter sequence which encodes transcripts, the expression of which are easily monitored in vitro and which result in a permanent genetic change, excision of the reporter gene, that is heritable and easily detectable subsequent to induction of the synthetic operon.
    Type: Grant
    Filed: February 27, 1995
    Date of Patent: November 5, 1996
    Inventors: John J. Mekalanos, Andrew Camilli
  • Patent number: 5512452
    Abstract: A reporter system relating to in vivo expression technology was devised to aid in the identification and study of genes that display temporal or spatial patterns of expression during infection of host tissues. The method of this invention comprises integrating a site-specific DNA recombinase expression vector, and a reporter gene that is permanently removable by the recombinase, by way of homologous recombination into a microorganism's chromosome and inducing the expression of a synthetic operon which encodes transcripts, the expression of which are easily monitored in vitro and which result in a permanent genetic change, excision of the reporter gene, that is heritable and easily detectable subsequent to induction of the synthetic operon.
    Type: Grant
    Filed: September 28, 1993
    Date of Patent: April 30, 1996
    Assignee: President and Fellows of Harvard College
    Inventors: John J. Mekalanos, Andrew Camilli
  • Patent number: 5434065
    Abstract: A genetic system termed in vivo expression technology was devised that positively selects for microbial genes that are specifically induced when microbes infect their host. The method of this invention comprises complementing the growth of an auxotrophic or antibiotic sensitive microorganism by integrating an expression vector by way of homologous recombination into the auxotrophic or antibiotic sensitive microorganism's chromosome and inducing the expression of a synthetic operon which encodes transcripts, the expression of which are easily monitored both in vitro and in vivo.
    Type: Grant
    Filed: May 6, 1993
    Date of Patent: July 18, 1995
    Assignee: President and Fellows of Harvard College
    Inventors: Michael J. Mahan, John J. Mekalanos, James M. Slauch
  • Patent number: 5330753
    Abstract: The gene encoding the TcpA pilus has been cloned. It encodes a protein useful in live, killed-cell, and synthetic vaccines. Protein production is enhanced by specific medium conditions.
    Type: Grant
    Filed: March 23, 1992
    Date of Patent: July 19, 1994
    Assignee: President and Fellows of Harvard College
    Inventors: John J. Mekalanos, Ronald K. Taylor
  • Patent number: 5098998
    Abstract: The gene encoding the TcpA pilus has been cloned. It encodes a protein useful in live, killed-cell, and synthetic vaccines. Protein production is enhanced by specific medium conditions.
    Type: Grant
    Filed: April 29, 1988
    Date of Patent: March 24, 1992
    Assignee: President and Fellows of Harvard College
    Inventors: John J. Mekalanos, Ronald K. Taylor
  • Patent number: 4882278
    Abstract: A genetically stable nontoxinogenic form of the Ogawa 395 strain of Vibrio cholerae which has a deletion mutation in both copies of the A.sub.1 -peptide-encoding gene, resulting in the loss of a gene sequence required for expression of a toxic A.sub.1 peptide is disclosed, as well as plasmids and methods for making the strain. The strain is useful as a live or dead oral vaccine.
    Type: Grant
    Filed: April 29, 1983
    Date of Patent: November 21, 1989
    Assignee: President and Fellows of Harvard College
    Inventor: John J. Mekalanos