Patents by Inventor John J. Wille, Jr.

John J. Wille, Jr. has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Protein-free defined media for the growth of normal human keratinocytes
    Patent number: 7037721
    Abstract: Improvements are made to a novel media that replace the requirement for all protein growth factors by the addition to the medium of physiological concentrations of retinyl acetate. The media are serum-free, companion cell or feeder layer-free and organotypic, matrix free solutions for the cultivation of clonally competent basal keratinocytes. The media and methods are useful in the production of epidermal epithelial tissue that is suitable for skin grafting.
    Type: Grant
    Filed: October 23, 2000
    Date of Patent: May 2, 2006
    Assignee: Hy-Gene Biomedical, Inc.
    Inventor: John J. Wille, Jr.
  • Hepes based basal nutrient medium for the isolation and culturing of stem cells
    Patent number: 6162643
    Abstract: Novel media and methods are disclosed for the growth of stem cells. The media are serum-free, companion cell or feeder layer free and organotypic, matrix free solutions for the isolation and cultivation of clonally competent basal epithelial cells. The media and methods of the invention are also useful in the production of epithelial tissues such as epidermis, cornea, gingiva and ureter.
    Type: Grant
    Filed: March 18, 1999
    Date of Patent: December 19, 2000
    Assignee: Hy-Gene Biomedical Corporation
    Inventor: John J. Wille, Jr.
  • Process and media for the growth of human cornea and gingiva
    Patent number: 5912175
    Abstract: Media and methods are disclosed for the in vitro formation of a histologically complete human epithelium. The media are serum-free, companion cell or feeder layer free and organotypic, matrix free solutions for the isolation and cultivation of clonally competent basal epithelial cells. The media and methods of the invention are useful in the production of epithelial tissues such as epidermis, cornea, gingiva and ureter.
    Type: Grant
    Filed: August 13, 1998
    Date of Patent: June 15, 1999
    Assignee: Hy-Gene, Inc.
    Inventor: John J. Wille, Jr.
  • Process and media for the growth of human epithelia
    Patent number: 5834312
    Abstract: Novel media and methods are disclosed for the in vitro formation of a histologically complete human epithelium. The media are serum-free, companion cell or feeder layer free and organotypic, matrix free solutions for the isolation and cultivation of clonally competent basal epithelial cells. The media and methods of the invention are useful in the production of epithelial tissues such as epidermis, cornea, gingiva and ureter.
    Type: Grant
    Filed: July 15, 1997
    Date of Patent: November 10, 1998
    Assignee: Hy-Gene, Inc.
    Inventor: John J. Wille, Jr.
  • Method for the formation of a histologically-complete skin substitute
    Patent number: 5292655
    Abstract: Methods and formulations are disclosed for the in vitro formation of a histologically complete human epidermis in a serum-free, companion cell or cell feeder layer-free, and organotypic matrix-free culture system commencing with the isolation and cultivation of a unique population of clonally-competent basal epidermal cells and ending with the formation of a functional, histologically complete, human squamous epithelium. The formation of a histologically complete human epidermis is accomplished in a serum-free medium, without companion-cells or feeder layer cells or any organotypic support using a multi-step process that is controlled by manipulating the growth and differentiation factors requisite to the sequential development of a usable, functional, and completely differentiated epidermis.
    Type: Grant
    Filed: January 29, 1990
    Date of Patent: March 8, 1994
    Inventor: John J. Wille, Jr.