Abstract: A crosslinked polymeric bead comprising a polymer having from 0.5 mole percent to 2 mole percent crosslinker. The bead has a diameter no greater than 200 ?m, no void spaces having a diameter greater than 5 ?m, and less than 5 weight percent of organic extractables.

Abstract: The present invention provides a method for producing a free flowing and/or non clumping resin that includes prior to drying, shrinking the resin under agitation. In one variant of the invention, the is a resin used in solid phase synthesis of peptides. In another aspect the invention provides a process of making a peptide using the free flowing resin described herein, and a polypeptide made using the process. Various therapeutics are then be made using the polypepdite.

Abstract: A method for functionalizing a crosslinked polymer bead. The method comprises the steps of: (a) swelling the bead in a first solvent or solvent mixture to a volume from 200% to 310% of its volume when dry; and (b) contacting the bead with a functionalizing reagent in a second solvent or solvent mixture capable of swelling the bead to a volume from 200% to 310% of its volume when dry.

Abstract: The present invention relates to an improved process for making commercial quantities of a polypeptide or fragment thereof, e.g. a T-20 or a T-1249 composition, or a fragment of a T-20 or a T-1249 composition. One variant of a T-20 composition is known as Fuzeon™ enfuvirtide. The improvement includes using a low void space resin, a resin made from a copolymer having less than 5% organic extractables, a resin made using a chloride corrosion resistant filter, resin beads functionalized using a nitro-compound, resins made using jetting techniques, and resins made using seed expansion techniques. In yet other variants, the invention provides a composition made using the processes described herein.

Abstract: The present invention relates to a process for making a biologically active substance or therapeutic agent free of a chlorotrityl chloride linker-resin. The process includes reacting an activated amino acid or activated amino acid derivative with a substituted or unsubstituted trityl alcohol resin to obtain a resin-CT-AA product; and, reacting the resin-CT-AA product with other building blocks of the biologically active substance or therapeutic agent to obtain said biologically active substance or therapeutic. A product created by this process is also provided herein. The invention is particularly useful to create T-20 and T-1249 therapeutics.

Abstract: The present invention provides a method for producing a free flowing and/or non clumping resin that includes prior to drying, shrinking the resin under agitation. In one variant of the invention, the is a resin used in solid phase synthesis of peptides. In another aspect the invention provides a process of making a peptide using the free flowing resin described herein, and a polypeptide made using the process. Various therapeutics are then be made using the polypepdite.

Abstract: A crosslinked polymer bead which, when: (i) functionalized with a 2-chlorotrityl chloride group; (ii) coupled with Leu to 0.65 mmol/g; and (iii) coupled with Glu(t-Bu); allows coupling of FMOC-Lys(BOC)-OH at an amount of 1.5 equivalents in the presence of 1.5 equivalents of HOBT, 1.5 equivalents of DIEA and 1.5 equivalents of HBTU, to be completed, as determined by the Kaiser test, in no more than 35 minutes.

Abstract: A method for functionalizing a crosslinked polymer bead. The method comprises the steps of: (a) swelling the bead in a first solvent or solvent mixture to a volume from 200% to 310% of its volume when dry; and (b) contacting the bead with a functionalizing reagent in a second solvent or solvent mixture capable of swelling the bead to a volume from 200% to 310% of its volume when dry.

Abstract: A crosslinked polymeric bead comprising a polymer having from 0.5 mole percent to 2 mole percent crosslinker. The bead has a diameter no greater than 200 &mgr;m, no void spaces having a diameter greater than 5 &mgr;m, and less than 5 weight percent of organic extractables.

Abstract: The present invention relates to a resin cleaning method using fewer solvents.

Abstract: An improved method for measuring the chromatographic properties, such as excluded volume, of hydrophobic polymer substrates is disclosed. Use of 0.05-1 micron sized crosslinked ionically-charged polymer particles, especially emulsion-form or ground polymer particles, as a large-molecule probe, allows environmentally friendly aqueous-based solvent systems to be used as mobile phases to characterize hydrophobic polymer supports for use in analytical or preparative scale chromatographic applications. This method eliminates the use of non-polar organic solvents that is required when conventional non-polar large-molecule probes (such as polystyrene) are used to characterize chromatographic media.

Abstract: Macroporous polymers having selected porosity and permeability characteristics that provide rigid polymer matrices suitable for use in medium and high pressure reversed phase liquid chromatography (RPC) are disclosed. A method for preparing the polymers using selected mixed porogens in selected proportions relative to the monomer phase is also disclosed. The polymers are especially useful as stationary phases in large scale chromatography columns without developing increased pressures during prolonged use, while maintaining good chromatographic performance for targeted biomolecules, such as insulin.

Abstract: Macroporous polymers having selected porosity and permeability characteristics that provide rigid polymer matrices suitable for use in medium and high pressure reversed phase liquid chromatography (RPC) are disclosed. A method for preparing the polymers using selected mixed porogens in selected proportions relative to the monomer phase is also disclosed. The polymers are especially useful as stationary phases in large scale chromatography columns without developing increased pressures during prolonged use, while maintaining good chromatographic performance for targeted biomolecules, such as insulin.

Abstract: An improved method for measuring the chromatographic properties, such as excluded volume, of hydrophobic polymer substrates is disclosed. Use of 0.05-1 micron sized crosslinked ionically-charged polymer particles, especially emulsion-form or ground polymer particles, as a large-molecule probe, allows environmentally friendly aqueous-based solvent systems to be used as mobile phases to characterize hydrophobic polymer supports for use in analytical or preparative scale chromatographic applications. This method eliminates the use of non-polar organic solvents that is required when conventional non-polar large-molecule probes (such as polystyrene) are used to characterize chromatographic media.