Abstract: The present invention is a DNA expression vector comprising: a toxP: a mutant toxO that blocks Fe-mediated regulation of gene expression; and a DNA sequence encoding a protein, wherein the toxP and the mutant toxO regulate expression of the DNA segment encoding the protein. It is preferred that DNA expression vectors of the present invention include DNA sequences encoding a signal peptide so that a protein expressed is attached to the signal peptide prior to processing. Novel proteins are produced off of the DNA expression vector of the present invention.

Abstract: An inflatable enclosure for amusement purposes and/or promoting physical activity comprising: inflatable means being formed of a plurality of interconnected panels of an inflatable material, taking the form of a chamber, when inflated, said chamber having, an interior, exterior, side walls, front wall, back wall , a floor and a ceiling whereon at least one panel thereof, there is a window of transparent material, blower means for supplying air at a predetermined rate and pressure to said inflatable means, blower aperture means disposed on one of said interconnected panels forming one of said side wall, to allow said blower means to be in contact with said inflatable means, at least one vent means located on each of said panels forming the side walls and the front walls, to provide venting of said air in said chamber of predetermined rate not causing substantial underinflation and/or overinflation, at least one amusement floating means disposed in the interior of said chamber, air actuated entrance/exit area s

Abstract: An inflatable enclosure for amusement purposes and/or promoting physical activity comprising:inflatable means forming a chamber when inflated,blower means for supplying air at a predetermined rate and at a predetermined pressure to inflate said inflatable means, to form a chamber,floating means located in said chamber, such that when said inflatable means is inflated by said blower means, said air supplied at a predetermined rate causes said floating means to float around in said chamber.