Abstract: The present invention provides a variety of related proteomics analytical modalities that are open-ended, rapid, convenient and suitable for implementation in a high throughput parallel assay system. Specificity-determining compositions and methods are disclosed for use in proteomics. These compositions and methods provide a protein resolved from other protein species contained in a sample fluid, in its native, biologically functional conformation. The present invention provides a specificity-determining substrate that forms a complex with a protein molecule in a homogenous fashion. The specificity-determining substrate includes a specificity-determining ligand bound to a support, wherein optionally the substrate further includes a spacer bound between the ligand and the support. In addition a complex is provided that includes a specificity-determining substrate and a protein molecule.

Abstract: A method for removing proteins and unwanted aggregated DNA from biological media containing nucleic acids by subjecting the starting material to a water insoluble complex consisting of ProCipitate™ and protein interspersed with ferric oxide particles to a magnetic force.

Abstract: There is provided water insoluble cross-linked polycarboxylic acid having at least two strands in a strand skeleton of the formula: ##STR1## wherein one carbonyl group of at least one maleoyl moiety thereof in each strand is covalently linked to a--HN.[(H).sub.p (CH).sub.2.(OH).sub.m ].NH-- moietyto provide the presence therein of at least one cross linked moiety of the formula: ##STR2## wherein R is hydrogen or lower alkylene or lower alkoxy of 1-4 carbon atoms, or phenyl, x and y are integers, z is an integer of 1-6, p is 0 or an integer up to z-1, m is 0 or an integer up to z, and w is an integer from 2-12. There is disclosed a method of making such polycarboxylic acid as well as methods of utilizing same to remove proteins from aqueous media containing same to provide a matrix. Methods are disclosed for recovering proteins from said matrix without denaturing them and methods for recovering the polycarboxylic acid from said matrix.

Abstract: There is provided a process for the preparation of a highly reactive hydrophobic silica capable of lipophilic binding of lipids present in aqueous media, in particular body fluids. The hydrocarbon substituted silica utilized in the first step of this process may be prepared from hydrophilic silica, suitably pyrogenic silica. This is then treated with a volatile hydrocarbon substituted silicon compound. The next step of the process comprises treating the substituted hydrophobic silica with an alcohol. In order to enable the thus produced material to selectively adsorb lipids in the presence of protein, to the substantial non absorption of the proteins (other than lipoproteins), a further step is carried out. This comprises treating with aqueous ammonia or amines wherein substituents on the amine nitrogen may be the same or different.

Abstract: A method of adsorbing from a solution comprising a biological sample viruses which retain their viability and infectivity. The method comprises adjusting the pH of said solution to pH 6.0 to 8.0; adding an effective amount of a water insoluble cross-linked polycarboxylic acid polymer ("WCPP") into said solution in a volume:volume ratio of WCPP to solution of 100:1 to 1:10,000 to form a WCPP-solution mixture; incubating said WCPP-solution mixture for a time sufficient to immobilize said viruses on said WCPP forming a WCPP-virus matrix; and separating said matrix from said solution. This novel method is suitable for removing, purifying, recovering and analyzing viable viruses as well as viral components such as viral proteins and nucleic acids.

Abstract: There is provided water insoluble cross-linked polyhydroxy polycarboxylic acid having at least two strands each having a strand skeleton of the formula: ##STR1## wherein one carbonyl group of at least one maleoyl moiety thereof in each strand is covalently linked to a--HN.[(H).sub.p (CH).sub.z.(OH).sub.m ].NH-- moietyto provide the presence therein of at least one cross linking moiety of the formula: ##STR2## wherein R is hydrogen or lower alkylene or lower alkoxy of 1-4 carbon atoms, or phenyl, z is an integer of 1-4, p is 0 or an integer up to z-1, m is 1 or an integer up to z, wherein the ratio of cross-links to poly (alkylene carbonic acid) strands is between about 1 and about 200 to 2. There is disclosed a method of making such polyhydroxy polycarboxylic acid as well as methods of utilizing same to remove proteins from aqueous media containing same to provide a matrix.

Abstract: There is provided water insoluble cross-linked polyhydroxy polycarboxylic acid having at least two strands each having a strand skeleton of the formula: ##STR1## wherein one carbonyl group of at least one maleoyl moiety thereof in each strand is covalently linked to a--HN.[(H).sub.p (CH).sub.z.(OH).sub.m ].NH-- moietyto provide the presence therein of at least one cross linking moiety of the formula: ##STR2## wherein R is hydrogen or lower alkylene or lower alkoxy of 1-4 carbon atoms, or phenyl, z is an integer of 1-4, p is 0 or an integer up to z-1, m is 1 or an integer up to z, wherein the ratio of cross-links to poly (alkylene carbonic acid) strands is between about 1 and about 200 to 2. There is disclosed a method of making such polyhydroxy polycarboxylic acid as well as methods of utilizing same to remove proteins from aqueous media containing same to provide a matrix.

Abstract: There is provided water insoluble cross-linked polyhydroxy polycarboxylic acid having at least two strands each having a strand skeleton of the formula: ##STR1## wherein one carbonyl group of at least one maleoyl moiety thereof in each strand is covalently linked to a--HN.[(H).sub.p (CH).sub.z.(OH).sub.m ].NH-- moietyto provide the presence therein of at least one cross linking moiety of the formula: ##STR2## wherein R is hydrogen or lower alkylene or lower alkoxy of 1-4 carbon atoms, or phenyl, z is an integer of 1-4, p is 0 or an integer up to z-1, m is 1 or an integer up to z, wherein the ratio of cross-links to poly (alkylene carbonic acid) strands is between about 1 and about 200 to 2. There is disclosed a method of making such polyhydroxy polycarboxylic acid as well as methods of utilizing same to remove proteins from aqueous media containing same to provide a matrix.

Abstract: Binding assay reagents for use in optical assay systems are disclosed. Assays, such as immunoassays, employing the reagent are also disclosed. The reagent is comprised of paired, associated polypeptides one of which has multiple optically active dye labels. The binding assay reagents exhibit enhanced binding activity over that of the dye labelled polypeptides alone and also enhance the sensitivity of the binding assay by providing increased amounts of optical label.

Abstract: Process for the determination of carcinoembryonic antigen (CEA) in a sample of serum or plasma. The process comprises contacting a slightly acidic buffered sample of the serum or plasma with hydrophilic silica at room temperature, separating the silica from the sample and carrying out a radioimmunoassay for carcinoembryonic antigen on said sample.

Abstract: At least two incompatible solutions are simultaneously charged into a container maintained at a temperature substantially below the freezing temperature of each solution in such predetermined amounts that the separate charges of solution freeze on the walls and are then lyophilized.

Abstract: This invention relates to immunologic compositions comprising pyruvic aldehyde stabilized erythrocytes sensitized with an antigen by coupling of the stabilized erythrocytes to the antigen with bifunctional molecules, and to methods for preparing these compositions. Also disclosed are antiserum compositions which may be utilized with the described stable, sensitized erythrocytes of this invention as reagents in hemagglutination tests, and methods for their preparation. The invention further comprises lyophilization of these compositions and siliconized vials for use with the reagents of this invention.

Abstract: A mixture of at least two liquid compositions, each having a material which is incompatible in the presence of moisture with a material in the other liquid composition, is cooled to a temperature slightly above its freezing point. The cooled mixture is charged into a container cooled substantially below the freezing point of the mixture so the mixture freezes instantly. The frozen mixture is lyophilized to provide a mass of dry matter.

Abstract: A method is disclosed for the purification of the beta subunit of human chorionic gonadotrophin. Also disclosed is an antiserum composition and method of producing same using spleen immunization to obtain antiserum compositions having high antibody titers.