Abstract: A process for sterilizing a solution and thereafter transferring it to a solution container for storage. A sterilizing filter, flexible tubing and the solution container are provided as a pre-sterilized unit with a label indicating that the unit is sterile. A source of solution to be transferred is connected to an inlet of the sterilizing filter, and the solution is sterilized by transferring it from the source and through the sterilizing filter to the container via the flexible tubing. The integrity of the filter is determined by introducing a pressurized gas into the filter and observing through a light-transmissive portion in the sterile unit for gas bubbles, which would indicate a loss of filter integrity and unit sterility.

Abstract: A vial and syringe connector assembly is provided for the sterile addition of fluid material to a sealed container having an injection-type access port. The assembly comprises a syringe connector which carries a hollow, injection needle having one pointed end for passing through such access port. The other needle end is blunt. An open mouthed vial for the fluid material has an elastomeric sealing piston disposed in the vial mouth and movable in sealing relation axially of the vial. A bore is also defined axially in the sealing piston of a size to receive the blunt end of the needle. An elastomeric diaphragm which is integral with the sealing piston occludes the bore. The vial and sealing piston are carried on the syringe connector with the blunt needle end positioned in the bore. The blunt needle end is used to rupture the diaphragm for fluid flow through the needle of the contents of the vial without accidental "coring" of the diaphragm and consequent obstruction of flow through the needle.