Abstract: A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.

Abstract: A method of inseminating an animal including flowing a stream of a population of sperm cells through a channel, differentiating the sperm cells into two subpopulations of X-chromosome containing sperm cells and Y-chromosome containing sperm cells, selecting a desired subpopulation, ablating an undesired subpopulation, and collecting both the subpopulations of sperm cells including the desired subpopulation and the ablated undesired subpopulation together, wherein the collected population of sperm cells is used to fertilize an egg.

Abstract: This disclosure concerns a method of providing a hydrophobic coating on a microfluidic chip that promotes the discrete flow of at least one liquid. It includes applying the hydrophobic coating onto an area of the microfluidic chip. The disclosure further includes a microfluidic chip that provides discrete flow of at least one liquid.

Abstract: An apparatus and method of identifying objects includes: a microfluidic chip in which are disposed a plurality of channels, the micro-fluidic chip including: a main fluid channel into which a sample fluid mixture of objects to be identified is introduced; a plurality of sheath fluid channels into which sheath fluids are introduced, the sheath fluids which orient the objects in the main fluid channel in a predetermined direction while still maintaining laminar flow in the main fluid channel; an interrogation apparatus which detects and interrogates the oriented objects in the main fluid channel; and a focused energy apparatus which performs an action on the objects.

Abstract: An apparatus and method of identifying objects includes: a microfluidic chip in which are disposed a plurality of channels, the microfluidic chip including: a main fluid channel into which a sample fluid mixture of objects to be identified is introduced; a plurality of sheath fluid channels into which sheath fluids are introduced, the sheath fluids which orient the objects in the main fluid channel in a predetermined direction while still maintaining laminar flow in the main fluid channel; an interrogation apparatus which detects and interrogates the oriented objects in the main fluid channel; and a focused energy apparatus which performs an action on the objects.

Abstract: An apparatus and method of identifying objects includes: a microfluidic chip in which are disposed a plurality of channels, the microfluidic chip including: a main fluid channel into which a sample fluid mixture of objects to be identified is introduced; a plurality of sheath fluid channels into which sheath fluids are introduced, the sheath fluids which orient the objects in the main fluid channel in a predetermined direction while still maintaining laminar flow in the main fluid channel; an interrogation apparatus which detects and interrogates the oriented objects in the main fluid channel; and a focused energy apparatus which performs an action on the objects.

Abstract: An apparatus and method of identifying objects includes: a microfluidic chip in which are disposed a plurality of channels, the microfluidic chip including: a main fluid channel into which a sample fluid mixture of objects to be identified is introduced; a plurality of sheath fluid channels into which sheath fluids are introduced, the sheath fluids which orient the objects in the main fluid channel in a predetermined direction while still maintaining laminar flow in the main fluid channel; an interrogation apparatus which detects and interrogates the oriented objects in the main fluid channel; and a focused energy apparatus which performs an action on the objects.

Abstract: A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.

Abstract: A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.

Abstract: A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels.

Abstract: A method and system are described for collating all avenues of communication through a single source. A user provides communication information to a service provider. The service provider associates this communication information with an identification code. The user then provides the identification code to new contacts instead of the user's communication information. When the contact tries to communicate with the user, the identification code directs the contact to the service provider. The service provider checks to see if the contact is permitted to communicate with the user. If permission is granted, the service provider routes the communication to the user, preferably in a format specified by the user.

Abstract: Method affecting the interior of human flesh, providing modulated power signal to at least one solenoid to create a modulated solenoid action delivered through a mechanical interface to the human flesh to create a modulated audio effect into the interior. Providing the modulated power signal may include receiving an audio signal to create the modulated power signal, which may include fetching a down-converted audio signal and the audio signal from a memory device and/or frequency-down-converting the audio signal to create the down-converted audio signal. Receiving the audio signal may further include solenoid amplifying the down-converted signal to create the modulated power signal. The modulated audio effect into the interior of the human flesh, the modulated power signal and the down-converted audio signal are products of this method. Apparatus implementing the solenoid amplifying, receiving the audio signal, frequency-down-converting the audio signal in a variety of configurations.

Abstract: A centrifugal device comprising: a rotor having a rotor shaft having a longitudinal rotor axis; a holder having an access end, and a holder axis; a location mechanism for locating the holder within the centrifugal device, such that the holder is rotatable about the rotor axis, with the holder axis forming a non-zero angle with the rotor shaft axis, the location mechanism accurately locating the access end of the holder within the centrifugal device, wherein the location mechanism comprises a holding pin having an axis that is substantially perpendicular to the rotor axis, the holding pin being rotatable about the rotor axis.

Abstract: A centrifugal device comprising: a rotor having a rotor shaft having a longitudinal rotor axis; a holder having an access end, and a holder axis; location means for locating the holder within the centrifugal device, such that the holder is rotatable about the rotor axis, with the holder axis forming a non-zero angle with the rotor shaft axis, the location means accurately locating the access end of the holder within the centrifugal device, wherein the location means comprises a holding pin having an axis that is substantially perpendicular to the rotor axis, the holding pin being rotatable about the rotor axis.

Abstract: Method affecting the interior of human flesh, providing modulated power signal to at least one solenoid to create a modulated solenoid action delivered through a mechanical interface to the human flesh to create a modulated audio effect into the interior. Providing the modulated power signal may include receiving an audio signal to create the modulated power signal, which may include fetching a down-converted audio signal and the audio signal from a memory device and/or frequency-down-converting the audio signal to create the down-converted audio signal. Receiving the audio signal may further include solenoid amplifying the down-converted signal to create the modulated power signal. The modulated audio effect into the interior of the human flesh, the modulated power signal and the down-converted audio signal are products of this method. Apparatus implementing the solenoid amplifying, receiving the audio signal, frequency-down-converting the audio signal in a variety of configurations.

Abstract: A method of preventing undesirable vehicle behavior in a vehicle torque control system that includes determining an expected axle torque based on a vehicle speed, a powerplant profile, an accelerator pedal position and/or a cruise control signal. The method also includes estimating an actual axle torque of a powerplant based on a powerplant maximum torque, a throttle position and powertrain inefficiencies and converting the expected axle torque to an expected acceleration and the actual axle torque to an actual acceleration based on a wheel radius and a road load. The method further includes regulating a torque output of a powerplant when a rate-of-change in a difference between the expected acceleration and the estimated actual acceleration is greater than a first threshold.

Abstract: A method and system for evaluating internal business investments by estimating decision-factor variations provides a powerful decision-making tool for deciding whether or not to invest in a particular internal business option or for selecting between business investment alternatives. The investment may be a capital investment, a program investment, personnel investment or other business strategy. Variations in cost, benefit, strategic alignment and risk values input to the business decision tool are used to adjust the business value of a business case. The variations can be estimated from data gathered from surveys of personnel rating a confidence or perceived variation from the input values, variations may be estimated in the computed business value due to interdependencies to other adopted or non-adopted business cases, or variations may be estimated based on actual historical collected data on business case performance.

Abstract: In an arrangement for a tandem cold mill in order to compensate for "speed effect," each stand has a roll force memory unit and an oil film roll force controller unit which operate together, and in conjunction with a tensiometer, to maintain a relatively constant roll gap during the acceleration and deceleration phases of the mill. In threading, in tailing out, and in a full run speed of the mill, the roll force memory unit is constantly operating to obtain a "lock on" roll force reference for the stand prior to the acceleration or deceleration phase. A roll force error signal, which is the difference between a roll force reference of the roll force memory unit and an instantaneous roll force, enters the oil film roll controller. A proportional integrator type controller in the oil film roll force controller unit changes the roll force error signal within a limit of +25% of the desired tension.

Abstract: A method for making delivery gauge corrections at low mill speeds during threading and tailing out of a workpiece by varying the interstand tension of the workpiece. The tension between the last two stands is used with a desired tension and the delivery AGC by tension mode of an existing delivery automatic gauge control (AGC) to change the speed of the downstream stands. During the threading and tailing out phases, the delivery AGC by speed is turned off and set to zero. In the full run phase, the interstand regulators are changed to a tension by roll gap mode, and an existing delivery automatic gauge control (AGC) by speed is used to provide a stand speed reference for the downstream stands. In the full run phase, the delivery (AGC) by tension is turned off an set to zero. In the tailing out phase optionally, the tension between each stand is used in a similar manner to provide a speed reference change for each stand immediately downstream from where a tension controller is located.