Patents by Inventor John M. Dubach
John M. Dubach has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 8765458Abstract: The invention provides coated sensors for detecting the presence of analytes. The sensor comprises one or more fluorescent sources, such as one or more quantum dots or one or more fluorescent dyes, a polymeric matrix, a surface coating, and one or more analyte sensing components. The surface coating may be a conformal polymeric film, permeable to the analyte, which may be deposited via a solventless process such as initiated chemical vapor deposition or photoinitiated chemical vapor deposition. The surface coating may increase the biocompatibility of the sensor, reduce nonspecific protein adsorption, and/or sequester functional sensor components within the sensor. The invention also provides methods for detecting the presence of an analyte with coated sensors of the invention.Type: GrantFiled: May 29, 2013Date of Patent: July 1, 2014Assignees: The Charles Stark Draper Laboratory, Inc., Massachusetts Institute of TechnologyInventors: Heather A. Clark, Karen K. Gleason, Salmaan Baxamusa, John M. Dubach
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Publication number: 20130260014Abstract: The invention provides coated sensors for detecting the presence of analytes. The sensor comprises one or more fluorescent sources, such as one or more quantum dots or one or more fluorescent dyes, a polymeric matrix, a surface coating, and one or more analyte sensing components. The surface coating may be a conformal polymeric film, permeable to the analyte, which may be deposited via a solventless process such as initiated chemical vapor deposition or photoinitiated chemical vapor deposition. The surface coating may increase the biocompatibility of the sensor, reduce nonspecific protein adsorption, and/or sequester functional sensor components within the sensor. The invention also provides methods for detecting the presence of an analyte with coated sensors of the invention.Type: ApplicationFiled: May 29, 2013Publication date: October 3, 2013Inventors: Heather A. Clark, Karen K. Gleason, Salmaan Baxamusa, John M. Dubach
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Patent number: 8470300Abstract: The invention provides coated sensors for detecting the presence of analytes. The sensor comprises one or more fluorescent sources, such as one or more quantum dots or one or more fluorescent dyes, a polymeric matrix, a surface coating, and one or more analyte sensing components. The surface coating may be a conformal polymeric film, permeable to the analyte, which may be deposited via a solventless process such as initiated chemical vapor deposition or photoinitiated chemical vapor deposition. The surface coating may increase the biocompatibility of the sensor, reduce nonspecific protein adsorption, and/or sequester functional sensor components within the sensor. The invention also provides methods for detecting the presence of an analyte with coated sensors of the invention.Type: GrantFiled: September 8, 2009Date of Patent: June 25, 2013Assignees: The Charles Stark Draper Laboratory, Inc., Massachusetts Institute of TechnologyInventors: Heather A. Clark, Karen K. Gleason, Salmaan Baxamusa, John M. Dubach
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Patent number: 8263358Abstract: The invention provides ion-selective sensors capable of selectively measuring ions, e.g., Na+, K+, Cl?, etc., in the cytosol of a single living cell. The sensor comprises one or more quantum dots or a fluorescent dye, a pH-sensitive dye, and optionally an ion-selective component such as an ionophore. These elements may, for example, be disposed in a polymer matrix. The polymer matrix comprises an internalizing moiety which enables the sensor to localize within the cytosol of a cell. The internalizing moiety comprises a small molecule or peptide such as an amine, antepennepedia, mastoparan, or melittin that react under acidic conditions to release a sensor from the confines of a endosome. Once in the cytosol the sensors may detect ionic analytes by selective ion extraction by the polymer, thereby inducing a pH change within the sensor which in turn changes the absorbance of the pH-sensitive dye. The change of absorbance may in turn attenuate the intensity of detectable emissions, e.g.Type: GrantFiled: October 14, 2008Date of Patent: September 11, 2012Assignee: The Charles Stark Draper Laboratory, Inc.Inventors: Heather A. Clark, Daniel I. Harjes, John M. Dubach
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Patent number: 8114662Abstract: The invention provides ion-selective sensors comprising quantum dots capable of selectively measuring ions, e.g., Na+, K+, Cl?, etc., in various environments, including in the cytosol of a living cell. Quantum dots are attractive probes for microscopy due to their photophysical advantages over fluorescent dyes, including prolonged photostability, brightness and quantum efficiency. In certain embodiments, a sensor comprises one or more quantum dots, a pH-sensitive dye, and optionally an ion-selective component such as an ionophore. These elements may, for example, be disposed in a polymer matrix. In certain embodiments, the sensors may detect ionic analytes by selective ion extraction by the polymer, thereby inducing a pH change within the sensor which in turn changes the absorbance of the pH-sensitive dye. The change of absorbance may in turn attenuate the intensity of detectable emissions, e.g., fluorescence, from the quantum dot by directly absorbing its fluorescence emission.Type: GrantFiled: July 31, 2007Date of Patent: February 14, 2012Assignee: The Charles Stark Draper Laboratory, Inc.Inventors: Heather A. Clark, Daniel I. Harjes, John M. Dubach
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Publication number: 20100221188Abstract: The invention provides coated sensors for detecting the presence of analytes. The sensor comprises one or more fluorescent sources, such as one or more quantum dots or one or more fluorescent dyes, a polymeric matrix, a surface coating, and one or more analyte sensing components. The surface coating may be a conformal polymeric film, permeable to the analyte, which may be deposited via a solventless process such as initiated chemical vapor deposition or photoinitiated chemical vapor deposition. The surface coating may increase the biocompatibility of the sensor, reduce nonspecific protein adsorption, and/or sequester functional sensor components within the sensor. The invention also provides methods for detecting the presence of an analyte with coated sensors of the invention.Type: ApplicationFiled: September 8, 2009Publication date: September 2, 2010Applicants: The Charles Stark Draper Laboratory, Inc., Massachusetts Institute of TechnologyInventors: Heather A. Clark, Karen K. Gleason, Salmaan Husain Baxamusa, John M. Dubach
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Publication number: 20090142274Abstract: The invention provides ion-selective sensors capable of selectively measuring ions, e.g., Na+, K+, Cl?, etc., in the cytosol of a single living cell. The sensor comprises one or more quantum dots or a fluorescent dye, a pH-sensitive dye, and optionally an ion-selective component such as an ionophore. These elements may, for example, be disposed in a polymer matrix. The polymer matrix comprises an internalizing moiety which enables the sensor to localize within the cytosol of a cell. The internalizing moiety comprises a small molecule or peptide such as an amine, antepennepedia, mastoparan, or melittin that react under acidic conditions to release a sensor from the confines of a endosome. Once in the cytosol the sensors may detect ionic analytes by selective ion extraction by the polymer, thereby inducing a pH change within the sensor which in turn changes the absorbance of the pH-sensitive dye. The change of absorbance may in turn attenuate the intensity of detectable emissions, e.g.Type: ApplicationFiled: October 14, 2008Publication date: June 4, 2009Applicant: The Charles Stark Draper Laboratory, Inc.Inventors: Heather A. Clark, Daniel I. Harjes, John M. Dubach
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Publication number: 20080131909Abstract: The invention provides ion-selective sensors comprising quantum dots capable of selectively measuring ions, e.g., Na+, K+, Cl?, etc., in various environments, including in the cytosol of a living cell. Quantum dots are attractive probes for microscopy due to their photophysical advantages over fluorescent dyes, including prolonged photostability, brightness and quantum efficiency. In certain embodiments, a sensor comprises one or more quantum dots, a pH-sensitive dye, and optionally an ion-selective component such as an ionophore. These elements may, for example, be disposed in a polymer matrix. In certain embodiments, the sensors may detect ionic analytes by selective ion extraction by the polymer, thereby inducing a pH change within the sensor which in turn changes the absorbance of the pH-sensitive dye. The change of absorbance may in turn attenuate the intensity of detectable emissions, e.g., fluorescence, from the quantum dot by directly absorbing its fluorescence emission.Type: ApplicationFiled: July 31, 2007Publication date: June 5, 2008Inventors: Heather A. Clark, Daniel I. Harjes, John M. Dubach