Abstract: A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, an allele specific primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and optionally an enzyme having 3??5? exonuclease activity when the primer is non-hydrolyzable, which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on such detection.

Abstract: The present invention describes methods of using terminal-phosphate-labeled nucleotides in the presence of a manganese salt to enhance their substrate properties towards various enzymes. Particularly described are methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases, in the presence of a manganese salt. Further provided are manganese complexes of terminal-phosphate-labeled nucleotides as well as terminal-phosphate-labeled nucleotides with new linkers with enhanced substrate properties.

Abstract: An improved wick-holder for mounting a felt lubricating wick is provided with a passive inertia-activated pump mounted for submersion in the axle-cap lubricant-reservoir at a level that ensures that for all levels of the reservoir, the pump inlet is submerged in the lubricant of the reservoir. In a first embodiment, the passive inertia-activated pump takes the form of a piston-type pump, while in a second embodiment, the passive inertia-activated pump takes the form of a diaphragm pump. In either embodiment, the inlet of the passive inertia-activated pump is submersed in the lubricant-reservoir, and the output of the passive inertia-activated pump is directed to the surface-interface between the felt wick and the axle journal surface-area to increase the lubrication thereof over and above that which normally ensues owing to the conventional capillary action of the felt wick.

Abstract: An improved sleeve bearing for locomotive traction motor that provides additional lubrication over and above that of the one central wick currently used. The sleeve bearing is provided with a recirculation system comprised of either at least two additional wicks located near the 6:00 operating location or multiple oil communication and delivery grooves passageways communicating with the oil flinger grooves placed for the purpose of collecting and providing a mechanism for providing additional lubrication for extending the oil-wetted envelope available for supporting the truck-axle journal in the axial direction.

Abstract: An improved wick-holder for mounting a felt lubricating wick of a lubricating system for lubricating the axle journal surface supported in a support bearing of a traction motor used in a locomotive is provided with a plurality of oil-reservoir louver pockets or splash-cups molded or pressed into the wick holder which store oil from the time that the oil reservoir was full or near its maximum height and which store splashed oil during normal operational use, and delay the run off of the oil to increase the oil saturation of the wick housed within the wick holder. The oil reservoir pockets are located above the minimum serviceable oil level, and are designed to improve and increase oil saturation of the wick. As a result of the increased oil saturation, the wick are, therefore, capable of delivering increased amounts of oil to the axle journal area.

Abstract: An anode for a rechargeable lithium cell comprises carbon nanotubes that contain within them an element that can form alloys or compounds reversibly with lithium over a range of compositions. The element within the nanotubes may be aluminum or tin. These carbon nanotubes are bound together to form a coherent layer with a polymeric binder, such as a polymer of viniylidene fluoride. A cell with such an anode should have improved capacity and improved reversibility, because the nanotubes provide a stabilizing framework for the alloy.

Abstract: A contour or profile of a bore of a locomotive traction-motor support bearing structure, where such profile preserves the currently center-loading of the upper load zone but moves inboardly the lower load zone to a more general central location. The profile of the bore for the support bearing, according to the invention, takes into account not only truck-axle bending due to locomotive weight, but also that from motor tilt through bearing clearances, and couple action on the axle from heavy radial loads on PE support bearing and adjacent axle gear. The bore is configured such that the upper surface is horizontal, but the lower surface slopes downwardly in the outboard direction at an angle based on a function dependent upon the three misalignment factors.

Abstract: An improved sleeve bearing for locomotive traction motor that provides additional lubrication over and above that of the one central wick currently used. The sleeve bearing is provided with a recirculation system comprised of either at least two additional wicks located near the 6:00 operating location or multiple oil communication and delivery grooves passageways communicating with the oil flinger grooves placed for the purpose of collecting and providing a mechanism for providing additional lubrication for extending the oil-wetted envelope available for supporting the truck-axle journal in the axial direction.

Abstract: A contour or profile of a bore of a locomotive traction-motor support bearing structure, where such profile preserves the currently center-loading of the upper load zone but moves inboardly the lower load zone to a more general central location. The profile of the bore for the support bearing, according to the invention, takes into account not only truck-axle bending due to locomotive weight, but also that from motor tilt through bearing clearances, and couple action on the axle from heavy radial loads on PE support bearing and adjacent axle gear. The bore is configured such that the upper surface is horizontal, but the lower surface slopes downwardly in the outboard direction at an angle based on a function dependent upon the three misalignment factors.

Abstract: A machine for forming filled sealed pouches from a continuously moving sealable web of material includes a first sealing station for forming a longitudinal seal along a side edge of the web, and a horizontal sealing station for forming a plurality of transverse seals in the web. The horizontal sealing station includes a pair of conveyors each carrying jaws that mate along a sealing path to form the transverse seals. A method of forming filled sealed pouches from a continuously moving sealable web of material is also described.

Abstract: The present invention describes methods of using terminal-phosphate-labeled nucleotides in the presence of a manganese salt to enhance their substrate properties towards various enzymes. Particularly described are methods of detecting a nucleic acid in a sample, based on the use of terminal-phosphate-labeled nucleotides as substrates for nucleic acid polymerases, in the presence of a manganese salt. Further provided are manganese complexes of terminal-phosphate-labeled nucleotides as well as terminal-phosphate-labeled nucleotides with new linkers with enhanced substrate properties.

Abstract: A method of characterizing a nucleic acid sample is provided that includes the steps of: (a) conducting a DNA polymerase reaction that includes the reaction of a template, an allele specific primer, at least one terminal phosphate-labeled nucleotide, DNA polymerase, and optionally an enzyme having 3′→5′ exonuclease activity when the primer is non-hydrolyzable, which reaction results in the production of labeled polyphosphate; (b) permitting the labeled polyphosphate to react with a phosphatase to produce a detectable species; (c) detecting the detectable species; and (d) characterizing the nucleic acid sample based on such detection.