Patents by Inventor John Mantlo

John Mantlo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method of efficient extraction of protein from cells
    Patent number: 9995745
    Abstract: Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.
    Type: Grant
    Filed: November 4, 2015
    Date of Patent: June 12, 2018
    Assignees: Arbor Vita Corporation, Becton, Dickinson and Company
    Inventors: Adrien P. Malick, Virginia M. Crews, Julie L. Rosales, Carrie S. Ferguson, Jeff H. Bruton, Robert J. Beadenkopf, John Mantlo
  • Method of protein extraction from cells
    Patent number: 9771398
    Abstract: Methods for producing a protein extract from cells, such as cells containing viral proteins, are provided. In general terms, the methods involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent, neutralizing the pH of the intermediate composition to produce the protein extract. Kits and compositions for practicing the subject methods are also provided.
    Type: Grant
    Filed: January 15, 2015
    Date of Patent: September 26, 2017
    Assignees: Arbor Vita Corporation, Becton, Dickinson and Company
    Inventors: Stephen Lovell, Lydia Blank, Virginia Crews, Nancy Hasse, Peter Lu, Johannes Schweizer, John Mantlo
  • METHOD OF EFFICIENT EXTRACTION OF PROTEIN FROM CELLS
    Publication number: 20160216258
    Abstract: Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.
    Type: Application
    Filed: November 4, 2015
    Publication date: July 28, 2016
    Inventors: Adrien P. Malick, Virginia M. Crews, Julie L. Rosales, Carrie S. Ferguson, Jeff H. Bruton, Robert J. Beadenkopf, John Mantlo
  • Method of efficient extraction of protein from cells
    Patent number: 9207240
    Abstract: Methods for producing a protein extract from cells, such as cells or cellular samples containing viral proteins, are provided. In general terms, the methods may involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent such as a polyoxyethylene alkyl ether, neutralizing the pH of the intermediate composition to produce the protein extract. Such methods can be used in conjunction with methods for detecting one or more target proteins in a sample, such as viral proteins. Systems, kits and compositions for practicing the subject methods are also provided.
    Type: Grant
    Filed: November 14, 2007
    Date of Patent: December 8, 2015
    Assignee: ARBOR VITA CORPORATION
    Inventors: Adrien P. Malick, Virginia M. Crews, Julie L. Rosales, Carrie S. Ferguson, Jeff H. Bruton, Robert J. Beadenkopf, John Mantlo
  • METHOD OF PROTEIN EXTRACTION FROM CELLS
    Publication number: 20150266928
    Abstract: Methods for producing a protein extract from cells, such as cells containing viral proteins, are provided. In general terms, the methods involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent, neutralizing the pH of the intermediate composition to produce the protein extract. Kits and compositions for practicing the subject methods are also provided.
    Type: Application
    Filed: January 15, 2015
    Publication date: September 24, 2015
    Inventors: Stephen Lovell, Lydia Blank, Virginia Crews, Nancy Hasse, Peter Lu, Johannes Schweizer, John Mantlo
  • Method of protein extraction from cells
    Patent number: 8962262
    Abstract: Methods for producing a protein extract from cells, such as cells containing viral proteins, are provided. In general terms, the methods involve: increasing the pH of the cells to a pH of at least about pH 10.0 to produce an intermediate composition, and then, in the presence of a non-ionic detergent, neutralizing the pH of the intermediate composition to produce the protein extract. Kits and compositions for practicing the subject methods are also provided.
    Type: Grant
    Filed: May 11, 2007
    Date of Patent: February 24, 2015
    Assignees: Arbor Vita Corporation, Becton, Dickinson and Company
    Inventors: Stephen Lovell, Lydia Blank, Virginia Crews, Nancy Hasse, Peter Lu, Johannes Schweizer, John Mantlo