Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

Abstract: A continuous alpha monitor includes an air intake mechanism, which in turn includes an air mover and an air flowrate monitor, an air intake prefilter that limits particulates in the air intake mechanism to an aerodynamic diameter of 10 microns or less, and a particle size detector mounted downstream of the air intake prefilter, the air particle size detector providing an airborne dust concentration and a first distribution of aerodynamic diameters of particulates in air passing the prefilter, the particulates including depleted uranium particulates. The monitor further includes a sample filter web that collects the particulates; a solid state detector that detects alpha radiation emitted by the collected particulates; a processor that executes machine instructions embodied on a non-transient computer-readable storage medium to compute a dust loading on the sample filter web; and the processor computes an indication of alpha concentration detected by the detector mechanism.

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

Abstract: A glass article comprising an alkali aluminosilicate glass that is formable by down-draw processes for example slot- and fusion-draw to thicknesses of 125 ?m or less and is capable of being chemically strengthened by ion-exchange to achieve a compressive stress at its surface of at least 950 MPa, in some embodiments at least about 1000 MPa, and in other embodiments at least about 1100 MPa. The high surface compressive stress allows the glass to retain net compression and thus contain surface flaws when the glass is subjected to bending around a tight radius. The glass may be used in foldable display applications.

Abstract: A continuous alpha monitor includes an air intake mechanism, which in turn includes an air mover and an air flowrate monitor, an air intake prefilter that limits particulates in the air intake mechanism to an aerodynamic diameter of 10 microns or less, and a particle size detector mounted downstream of the air intake prefilter, the air particle size detector providing an airborne dust concentration and a first distribution of aerodynamic diameters of particulates in air passing the prefilter, the particulates including depleted uranium particulates. The monitor further includes a sample filter web that collects the particulates; a solid state detector that detects alpha radiation emitted by the collected particulates; a processor that executes machine instructions embodied on a non-transient computer-readable storage medium to compute a dust loading on the sample filter web; and the processor computes an indication of alpha concentration detected by the detector mechanism.

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

Abstract: A continuous alpha monitor includes an air intake mechanism, which in turn includes an air mover, an air intake prefilter that limits particulates in the air intake mechanism to an aerodynamic diameter of 10 microns or less, and a particle detector mounted downstream of the air intake prefilter, the air particle detector providing a first airborne dust concentration and a first distribution of aerodynamic diameters of particulates in air passing the prefilter, the particulates including depleted uranium particulates. The monitor further includes a movable sample filter web that collects the particulates; a solid state detector that detects alpha radiation emitted by the collected particulates; a processor that executes machine instructions embodied on a non-transient computer-readable storage medium to compute a dust loading on the sample filter web; and the processor computes an indication of alpha concentration detected by the solid state detector.

Abstract: A continuous alpha monitor includes an air intake mechanism, which in turn includes an air mover and an air flowrate monitor, an air intake prefilter that limits particulates in the air intake mechanism to an aerodynamic diameter of 10 microns or less, and a particle size detector mounted downstream of the air intake prefilter, the air particle size detector providing a distribution of aerodynamic diameters of particulates in air passing the prefilter, the particulates including depleted uranium particulates. The monitor further includes a sample filter mechanism that collects the particulates; a detector mechanism that detects alpha radiation emitted by the collected particulates; a dust loading mechanism that computes a dust thickness on the sample filter mechanism; and an output mechanism that provides an indication of alpha concentration detected by the detector mechanism.

Abstract: Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. Nanoparticle compositions resulting from these methods are also provided.

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

Abstract: Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. Nanoparticle compositions resulting from these methods are also provided.

Abstract: Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. Nanoparticle compositions resulting from these methods are also provided.

Abstract: A nanoparticle having one or more phosphonates or phosphinate ligands on their surface can be converted into nanoparticles with sulfonate ligands on their surface, by contacting the phosphonate-containing nanoparticles with a silylsulfonate reagent. Such nanoparticles are activated toward reactions with nucleophilic groups; thus the method provides activated nanoparticles, and methods of making and using them to produce modified nanoparticles.

Abstract: Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are polymerase-nanoparticle conjugates including a polymerase linked to a nanoparticle, wherein the conjugate has polymerase activity. Such conjugates can exhibit reduced aggregation and improved stochiometries wherein the average biomolecule:nanoparticle ratio approaches or equals 1:1. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing.

Abstract: Provided is an optical in vivo contrast agent comprising a fluorescent polymeric microsphere, wherein the micro-sphere is impregnated with a dye having an excitation and emission spectrum compatible with in vivo imaging, and wherein the microsphere is coated with a block copolymer.

Abstract: Provided is reagents and methods for non-invasive in vivo imaging wherein the reagents comprise targeted carrier molecules conjugated to a NIB reporter molecule. In one aspect the targeted carrier molecule is an antibody, or fragment thereof that has specificity for an antigen in a living body, animal or human. In one embodiment the antibodes are anti-cancer/tumor marker antibodies, organ specific antibodies, tissue specific antibodies, cell type specific antibodies, cell surface specific antibodies, anti-viral antibodies, anti-bacterial antibodies and anti-pathogenic antibodies. The NIP reporter molecules are any fluorescent reporter molecule compatible with in vivo imaging and generally having an excitation wavelength of at least 580 nm.

Abstract: The described embodiments may provide a method of fabricating a chemical detection device. The method may comprise forming a microwell above a CMOS device. The microwell may comprise a bottom surface and sidewalls. The method may further comprise applying a first chemical to be selectively attached to the bottom surface of the microwell, forming a metal oxide layer on the sidewalls of the microwell, and applying a second chemical to be selectively attached to the sidewalls of the microwell. The second chemical may lack an affinity to the first chemical.

Abstract: Provided herein are methods for making water-soluble nanoparticles comprising a core/shell nanocrystal that is coated with a surface layer comprising enough hydrophilic ligands to render the nanoparticle water soluble or water dispersable. Methods for crosslinking molecules on the surface of a nanoparticle, which methods can be used on the above water-soluble nanoparticles also are provided. Nanoparticle compositions resulting from these methods are also provided.

Abstract: A nanoparticle having one or more phosphonates or phosphinate ligands on their surface can be converted into nanoparticles with sulfonate ligands on their surface, by contacting the phosphonate-containing nanoparticles with a silylsulfonate reagent. Such nanoparticles are activated toward reactions with nucleophilic groups; thus the method provides activated nanoparticles, and methods of making and using them to produce modified nanoparticles.