Abstract: The invention relates to proteinase inhibitors and in particular cystatins which have been modified so as to enhance the effectiveness of synthetically manufactured counterparts. The modifications include either site-directed alterations in the structure of the protein and/or the production of hybrid molecules.

Abstract: The invention relates to proteinase inhibitors and in particular cystatins which have been modified so as to enhance their effective or synthetically manufactured counterparts. The modification including either site-directed alterations in the structure of the protein and/or the production of hybrid molecules.

Abstract: A method for combating crop pests or animal parasites is provided by contacting them with a peptide which has a molecular weight of 12,000 Dalton or less, which is able to exert its effect after entry into the neurons of the crop pest or in the synaptic cleft and which is able to be taken up through retrograde neuronal transport starting from a sense organ.

Abstract: This invention seeks to provide means by which transgenic plants harbour within their genome a chimaeric gene which when expressed produces a protein having a cytotoxic effect. Cytotoxicity is achieved by way of a protein or part thereof capable of blocking cellular protein synthesis, such as a maize ribosome inactivating protein or part thereof. The chimaeric gene may comprise a promoter operably linked to a sequence encoding a protein capable of blocking cellular protein synthesis such that the promoter is inducible at and/or adjacent to a target site.

Abstract: This invention seeks to provide means by which a necrotic effect may be induced in specific cells of a plant. The plant is transformed with a chimaeric gene which encodes a pokeweed antiviral protein and a promoter which acts in response to a specific stimulus so as to facilitate expression of the pokeweed antiviral protein in specific cells of the plant.

Abstract: Disclosed herein is a substantially pure nucleic acid sequence encoding a mammalian 35 kDa non-dystrophin component (&dgr;-sarcoglycan) of the dystrophin-glycoprotein complex. Also disclosed are the amino acid sequence and an immunogenic peptide of &dgr;-sarcoglycan. The peptide when used to immunize a mammal, stimulates the production of antibodies which bind specifically to the &dgr;-sarcoglycan. Methods to identify mutations in the &dgr;-sarcoglycan gene associated with autosomal recessive limb-girdle muscular dystrophy are also disclosed. The identification of such mutations enables the design of nucleic acid probes which hybridize specifically to a mutant form of &dgr;-sarcoglycan, or the complement thereof, but not to the DNA of the wild-type form of the gene (or the complement thereof), under stringent hybridization conditions. Such probes are useful, for example, in connection with the diagnosis of autosomal recessive limb-girdle muscular dystrophy.

Abstract: Disclosed herein is a substantially pure nucleic acid sequence encoding a mammalian 35 kDa non-dystrophin component (.delta.-sarcoglycan) of the dystrophin-glycoprotein complex. Also disclosed are the amino acid sequence and an immunogenic peptide of .delta.-sarcoglycan. The peptide when used to immunize a mammal, stimulates the production of antibodies which bind specifically to the .delta.-sarcoglycan. Methods to identify mutations in the .delta.-sarcoglycan gene associated with autosomal recessive limb-girdle muscular dystrophy are also disclosed. The identification of such mutations enables the design of nucleic acid probes which hybridize specifically to a mutant form of .delta.-sarcoglycan, or the complement thereof, but not to the DNA of the wild-type form of the gene (or the complement thereof), under stringent hybridization conditions. Such probes are useful, for example, in connection with the diagnosis of autosomal recessive limb-girdle muscular dystrophy.

Abstract: A method of controlling nematodes, the method includes the steps of identification of a gene induced within a successfully infected plant by nematode infection of said plant and modifying the gene to confer nematode resistance to the plant.

Abstract: The present invention provides an evaluation strategy for the execution of SQL queries involving recursion and table queues in a relational database management system. The table queue is established for the query in the memory of the computer, and tuples are generated by tuple-producing operators in the query for storage into the table queue. As operators of the query read the stored tuples from the table queue, a first end-of-file condition, termed a Temporary End-Of-File, occurs when all of the tuples currently stored in the table queue have been retrieved and control is returned to the operator reading from the table queue. A retry request for additional tuples is generated by the operator through the table queue to the tuple-producing operators. If the tuple-producing operators can generate additional tuples, then these additional tuples are stored into the table queue and control is returned to the operator reading from the table queue in response to the retry request.

Abstract: Cross-linked atelopeptide collagen that is substantially free of residual cross-linking agent is prepared by: reconstituting atelopeptide collagen from solution by neutralizing the solution at a reduced temperature and a hypotonic ionic strength; cross-linking the reconstituted fibers in an aqueous medium at a concentration of 0.1 to 10 mg/ml with glutaraldehyde under conditions that produce cross-linked collagen that when in suspension in physiological saline at a concentration of 35 mg/ml exhibits a shear viscosity whose log varies linearly with the log of the shear rate and is approximated by the formulalog .eta..ltoreq.-0.96 log .gamma.+2.3where .gamma. is the shear rate in sec.sup.-1, log .gamma. is in the range of -6 to +2 and .eta. is the viscosity of the suspension in Pascal-sec; optionally quenching the cross-linking reaction with an amino acid; and separating the cross-linked atelopeptide collagen from the reaction mixture.