Abstract: This invention is directed to preparation and expression of synthetic genes encoding polypeptides containing protective epitopes of botulinum neurotoxin (BoNT). The invention is also directed to production of immunogenic peptides encoded by the synthetic genes, as well as recovery and purification of the immunogenic peptides from recombinant organisms. The invention is also directed to methods of vaccination against botulism using the expressed peptides.

Abstract: An apparatus for the delivery of toxicant to vermin includes an expandable conduit having an entry and exit for vermin, and which is expandable upon installation into an area determined to be suitable for vermin control. A toxicant is placeable in the expanded conduit such that vermin will contact the toxicant when passing through the conduit. In another apparatus, a toxicant is disposed within a conduit having an entry and exit for vermin. The toxicant is secured within the conduit to prevent spillage of the toxicant upon installation of the apparatus. The toxicant may be preloaded in a container. Methods for using the apparatuses are also described.