Abstract: A latch device is provided with a driver and a shadow latch. The driver has an input to accept a binary driver input signal, an input to accept a clock signal, and an input to accept a shadow-Q signal. The driver has an output to supply a binary Q signal equal to the inverse of the driver input signal, in response to the driver input signal, the shadow-Q signal, and the clock signal. The shadow latch has an input to accept the driver input signal, and an input to accept the clock signal. The shadow latch has an output to supply the shadow-Q signal equal to the inverted Q signal, in response to the driver input signal and clock signal.

Abstract: Disclosed herein is a pseudo single-phase flip-flop. The master section includes a pre-dissipation stage and a first keeper. The pre-dissipation stage discharges the first keeper to the mDb second binary value, and selectively charges the first keeper with the mDb first binary value in the master pass mode. The pre-dissipation stage selectively prevents the first keeper from charging to the mDb first binary value in response to one of the clock phases. The slave section includes a pre-charge stage, a second keeper, a post-dissipation stage, and a third keeper. The second keeper maintains a first binary value in a slave pass mode when the mDb signal has a second binary value. The second keeper supports the second binary value in the slave pass mode when the mDb signal has the first binary value. The third keeper maintains the Q signal binary value during the slave hold mode.

Abstract: A latch device is provided with a relay and a shadow latch. The relay has an input to accept a binary relay input signal, an input to accept a clock signal, an input to accept a shadow-Q signal, and an output to supply a binary Q signal value equal to the relay input signal value. The relay output is supplied in response to the relay input signal, the shadow-Q signal, and the clock signal. The shadow latch has an input to accept the relay input signal, an input to accept the clock signal, and an output to supply the shadow-Q signal with a value equal to an inverted Q signal value. The shadow latch output is supplied in response to the relay input signal and clock signal.

Abstract: A hazard-free minimal-latency flip-flop (HFML-FF) is provided. A master latch includes an input to accept a D1 signal, an input to accept a clock signal, an input to accept an inverted shadow-D2 signal, and an output to supply a D2 signal. The master latch has an input to accept a shadow-D1 signal, an input to accept the clock signal, and an output to supply a shadow-D2 signal and the inverted shadow-D2 signal. The slave latch has an input to accept the D2 signal, an input to accept the clock signal, an input to accept an inverted shadow-Q signal, and an output to supply a Q signal. The slave latch has an input to accept either the D2 signal or the shadow-D2 signal, an input to accept the clock signal, and an output to supply a shadow-Q signal and the inverted shadow-Q signal. The design may use clocked inverters or pass gates.

Abstract: The invention provides methods and compositions for normalizing and amplifying RNA populations. The methods generally comprise the steps of copying message RNA (mRNA) to form first single-stranded (ss) cDNA; converting the first ss-cDNA to first double-stranded (ds) cDNA; linearly amplifying the first ds-cDNA to form first amplified RNA (aRNA); tagging the 3? end of the first aRNA with a known sequence to form 3?-tagged first aRNA; copying the 3?-tagged first aRNA to form second ss-cDNA; and normalizing the mRNA and/or the first aRNA.

Abstract: The invention provides methods and compositions for defining a cell type, generally involving the steps of (a) amplifying the mRNA of a single cell of a heterogenous population of cells; (b) probing a comprehensive expression library with the amplified mRNA to define a gross expression profile of the cell; and (c) comparing the gross expression profile of the cell with a gross expression profile of one or more other cells to define a unique expression profile of the cell, wherein the unique expression profile of the cell provides a marker defining the cell type

Abstract: Nucleic acids are made by converting a primed single-stranded DNA to a double-stranded DNA by a method comprising the step contacting the single-stranded DNA with a DNA polymerase having 5′ exonuclease activity under conditions whereby the DNA polymerase converts the-single stranded DNA to the double-stranded DNA, wherein the single-stranded DNA is primed with oligonucleotide primer comprising a sequence complementary to the 3′ end of the single-stranded DNA, and at least one of the 5′ end of the primer and the single-stranded DNA comprises an RNA polymerase promoter joined to an upstream (5′) flanking moiety which protects the promoter from the 5′ exonuclease activity of the DNA polymerase.

Abstract: The invention provides methods and compositions relating to odorant receptors, including a general expression cloning methodology which is useful for identifying novel G protein-coupled receptors and a novel family of odorant receptors and related nucleic acids, ligands, agonists and antagonists. These compositions provide a wide variety of applications such as screening for related receptors, and by modulating the function of the disclosed receptors by modulating their expression or contacting them with agonists, antagonist or ligands modulating reproductive/sexual and non-sexual social behaviors mediated via the olfactory system, reproductive physiologies and olfactory system regulated feeding behaviors, migratory behaviors and events such as conception, implantation, estrous and menstruation.

Abstract: The invention provides methods and compositions for defining a cell type, generally involving the steps of (a) amplifying the mRNA of a single cell of a heterogenous population of cells; (b) probing a comprehensive expression library with the amplified mRNA to define a gross expression profile of the cell; and (c) comparing the gross expression profile of the cell with a gross expression profile of one or more other cells to define a unique expression profile of the cell, wherein the unique expression profile of the cell provides a marker defining the cell type

Abstract: The invention provides methods and compositions relating to odorant receptors, including a general expression cloning methodology which is useful for identifying novel G protein-coupled receptors and a novel family of odorant receptors and related nucleic acids, ligands, agonists and antagonists. These compositions provide a wide variety of applications such as screening for related receptors, and by modulating the function of the disclosed receptors by modulating their expression or contacting them with agonists, antagonist or ligands modulating reproductive/sexual and non-sexual social behaviors mediated via the olfactory system, reproductive physiologies and olfactory system regulated feeding behaviors, migratory behaviors and events such as conception, implantation, estrous and menstruation.

Abstract: Nucleic acids are made by adding a known nucleotide sequence to the 3' end of a first RNA having a known sequence at the 5' end to form a second RNA and reverse transcribing the second RNA to form a cDNA. In one embodiment, the first RNA is an amplified mRNA, the known sequence at the 5' end comprises a poly(T) sequence, the adding step comprises using a polyadenyltransferase to add a poly(A) sequence to the 3' end, the reverse transcribing step is initiated at a duplex region comprising the poly(T) sequence hybridized to the poly(A) sequence, the cDNA is converted to double-stranded cDNA by a polymerase initiating from a noncovalently joined duplex region, and the double-stranded cDNA is transcribed to form one or more third RNAs.

Abstract: The invention provides methods and compositions for defining a cell type, generally involving the steps of (a) amplifying the mRNA of a single cell of a heterogenous population of cells; (b) probing a comprehensive expression library with the amplified mRNA to define a gross expression profile of the cell; and (c) comparing the gross expression profile of the cell with a gross expression profile of one or more other cells to define a unique expression profile of the cell, wherein the unique expression profile of the cell provides a marker defining the cell type.

Abstract: A pull-up or pull-down transistor in an output buffer or the like is controlled to limit dI/dt and thus reduce noise. This control employs a series transistor in the driver circuit which has a modulating voltage applied to its gate. This modulating voltage is generated in a circuit which uses precision-timed clocks as a reference so that variations in electrical parameters (process, temperature and power supply dependent) will not cause variation in circuit noise. A dummy driver circuit is used to discharge a capacitor at a rate dependent upon the modulating voltage, and the capacitor voltage is compared with a reference at a time determined by the precision clock. The result of the comparison is used to increment or decrement the modulating voltage.