Abstract: The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, 1,4-butanediol, or other product pathway and being capable of producing 4-hydroxybutyrate, 1,4-butanediol, or other product, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, 1,4-butanediol, or other product or related products using the microbial organisms.

Abstract: The instant invention is directed to a stopper rod spatial control mechanism for use in combination with a bottom discharge holding reservoir for molten metal having a nozzle. The mechanism comprises a base, an outer tube and an inner tube. The outer tube has a longitudinal axis which is perpendicular to the base. The outer tube is swingably mounted about an axis parallel to and spaced away from the longitudinal axis. The inner tube is telescopically mounted within the outer tube. The inner tube is reciprocally movable along the longitudinal axis and is rotatable about the longitudinal axis. A servomotor is mounted at a lower end of the outer tube. A ball screw is connected to and rotated by the motor. A ball nut is affixed to the inner tube and cooperates with the ball screw. A boom having a first end and a second end is affixed to the inner tube adjacent its first end and rotates with the inner tube about the longitudinal axis. A stopper rod depends from the second end of the boom.