Abstract: The invention relates generally to multispecific polypeptides having constrained CD3 binding. In some embodiments, the multispecific polypeptides contain cleavable linkers that, when cleaved, results in dual effector functions. Also provided are methods of making and using these multispecific polypeptides in a variety of therapeutic, diagnostic and prophylactic indications.

Abstract: An injection device includes a housing having a proximal end and a distal end, the housing defining a distal opening, and a first opening in a side of the housing and between the proximal and distal ends; a propellant disposed inside the housing and spaced from the distal end; and a movable member disposed inside the housing and between the distal end and the propellant, wherein the propellant is in fluid communication with the movable member and the first opening.

Abstract: Injection devices comprising multiple sections, e.g., modular components, are disclosed. In some embodiments the injection system comprises an upper section comprising a compressed gas reservoir, and one or more lower sections, e.g., modular components, containing one or more compositions to be injected. Methods of making and using the injection devices are also disclosed.

Abstract: An in vivo biosensor disposed upon a subject comprising an electrochemical cell having a plurality of electrodes and a computer-controlled voltage source incorporating a potentiostat that is generative of a poise potential regime, which computer-controlled voltage source is operationally coupled to a computing device that: computes an output current whose magnitude is proportional to an amount of an analyte in a bodily fluid of the subject; and, adjusts the output current for drift due to biofouling at points in time greater than or equal to an induction period; and, outputs the amount of the analyte by transducing the adjusted output current. Methods and algorithms for adjusting the output current for drift due to biofouling are provided.

Abstract: Electroactive pores, devices including one or more electroactive pores are described, and methods of delivering therapeutic agents using one or more electroactive pores are described.

Abstract: An injection device includes a housing having a proximal end and a distal end, the housing defining a distal opening, and a first opening in a side of the housing and between the proximal and distal ends; a propellant disposed inside the housing and spaced from the distal end; and a movable member disposed inside the housing and between the distal end and the propellant, wherein the propellant is in fluid communication with the movable member and the first opening.

Abstract: This invention provides methods and apparatus for performing microanalytic and microsynthetic analyses and procedures. Specifically, the invention provides a microsystem platform for use with a micromanipulation device to manipulate the platform by rotation, thereby utilizing the centripetal force resulting from rotation of the platform to motivate fluid movement through microchannels embedded in the microplatform. The microsystem platforms of the invention are also provided having microfluidics components, resistive heating elements, temperature sensing elements, mixing structures, capillary and sacrificial valves, and methods for using these microsystems platforms for performing biological, enzymatic, immunological and chemical assays. An electronic spindle designed rotor capable of transferring electrical signals to and from the microsystem platforms of the invention is also provided.

Abstract: This invention provides methods and apparatus for performing microanalytic and microsynthetic analyses and procedures. Specifically, the invention provides a microsystem platform for use with a micromanipulation device to manipulate the platform by rotation, thereby utilizing the centripetal force resulting from rotation of the platform to motivate fluid movement through microchannels embedded in the microplatform. The microsystem platforms of the invention are also provided having microfluidics components, resistive heating elements, temperature sensing elements, mixing structures, capillary and sacrificial valves, and methods for using these microsystems platforms for performing biological, enzymatic, immunological and chemical assays. An electronic spindle designed rotor capable of transferring electrical signals to and from the microsystem platforms of the invention is also provided.

Abstract: Electroactive pores, devices including one or more electroactive pores are described, and methods of delivering therapeutic agents using one or more electroactive pores are described.

Abstract: This invention provides methods and apparatus for performing microanalytic and microsynthetic analyses and procedures. Specifically, the invention provides a microsystem platform for use with a micromanipulation device to manipulate the platform by rotation, thereby utilizing the centripetal force resulting from rotation of the platform to motivate fluid movement through microchannels embedded in the microplatform. The microsystem platforms of the invention are also provided having microfluidics components, resistive heating elements, temperature sensing elements, mixing structures, capillary and sacrificial valves, and methods for using these microsystems platforms for performing biological, enzymatic, immunological and chemical assays. An electronic spindle designed rotor capable of transferring electrical signals to and from the microsystem platforms of the invention is also provided.

Abstract: An injection device includes a housing having a proximal end and a distal end, the housing defining a distal opening, and a first opening in a side of the housing and between the proximal and distal ends; a propellant disposed inside the housing and spaced from the distal end; and a movable member disposed inside the housing and between the distal end and the propellant, wherein the propellant is in fluid communication with the movable member and the first opening.

Abstract: This system provides methods and apparatus for performing microanalytic and microsynthetic analyses and procedures. Specifically, the system provides a microsystem platform for use with a micromanipulation device to manipulate the platform by rotation, thereby utilizing the centripetal force resulting from rotation of the platform to motivate fluid movement through microchannels embedded in the microplatform. The microsystem platforms of the system are also provided having microfluidics components, resistive heating elements, temperature sensing elements, mixing structures, capillary and sacrificial valves, and methods for using these microsystems platforms for performing biological, enzymatic, immunological and chemical assays. An electronic spindle designed rotor capable of transferring electrical signals to and from the microsystem platforms of the system is also provided.

Abstract: This invention provides methods and apparatus for performing microanalytic and microsynthetic analyses and procedures. Specifically, the invention provides a microsystem platform for use with a micromanipulation device to manipulate the platform by rotation, thereby utilizing the centripetal force resulting from rotation of the platform to motivate fluid movement through microchannels embedded in the microplatform. The microsystem platforms of the invention are also provided having microfluidics components, resistive heating elements, temperature sensing elements, mixing structures, capillary and sacrificial valves, and methods for using these microsystems platforms for performing biological, enzymatic, immunological and chemical assays. An electronic spindle designed rotor capable of transferring electrical signals to and from the microsystem platforms of the invention is also provided.

Abstract: An injection device includes a housing having a proximal end and a distal end, the housing defining a distal opening, and a first opening in a side of the housing and between the proximal and distal ends; a propellant disposed inside the housing and spaced from the distal end; and a movable member disposed inside the housing and between the distal end and the propellant, wherein the propellant is in fluid communication with the movable member and the first opening.

Abstract: This invention provides methods and apparatus for performing microanalytic and microsynthetic analyses and procedures. Specifically, the invention provides a microsystem platform for use with a micromanipulation device to manipulate the platform by rotation, thereby utilizing the centripetal force resulting from rotation of the platform to motivate fluid movement through microchannels embedded in the microplatform. The microsystem platforms of the invention are also provided having microfluidics components, resistive heating elements, temperature sensing elements, mixing structures, capillary and sacrificial valves, and methods for using these microsystems platforms for performing biological, enzymatic, immunological and chemical assays. An electronic spindle designed rotor capable of transferring electrical signals to and from the microsystem platforms of the invention is also provided.

Abstract: This invention provides methods and apparatus for performing microanalytic and microsynthetic analyses and procedures. Specifically, the invention provides a microsystem platform for use with a micromanipulation device to manipulate the platform by rotation, thereby utilizing the centripetal force resulting from rotation of the platform to motivate fluid movement through microchannels embedded in the microplatform. The microsystem platforms of the invention are also provided having microfluidics components, resistive heating elements, temperature sensing elements, mixing structures, capillary and sacrificial valves, and methods for using these microsystems platforms for performing biological, enzymatic, immunological and chemical assays. An electronic spindle designed rotor capable of transferring electrical signals to and from the microsystem platforms of the invention is also provided.

Abstract: This invention provides methods and apparatus for performing microanalytic and microsynthetic analyses and procedures. Specifically, the invention provides a microsystem platform for use with a micromanipulation device to manipulate the platform by rotation, thereby utilizing the centripetal force resulting from rotation of the platform to motivate fluid movement through microchannels embedded in the microplatform. The microsystem platforms of the invention are also provided having microfluidics components, resistive heating elements, temperature sensing elements, mixing structures, capillary and sacrificial valves, and methods for using these microsystems platforms for performing biological, enzymatic, immunological and chemical assays. An electronic spindle designed rotor capable of transferring electrical signals to and from the microsystem platforms of the invention is also provided.

Abstract: A point-of-care analyzer unit characterized by a disposable sensor module, a capability of cooperation with multiple fluid sources for providing multi-point calibration, which sources are detachably connectable with the sensor module, and a waste reservoir integral with the sensor module operable to receive and house multiple sets of complete test sequences comprising multiple calibration fluids sets and an analyte sample.

Abstract: A sensor and a method are disclosed for the potentiometric determination of the ion content or activity of a sample and the concentration of other components (e.g., glucose, urea, triglycerides, enzymes, drugs) of a sample. The sensor comprises ion-selective electrodes which are held in a frame and have porous material between them; the porous material provides a means to establish ionic flow between the electrodes. Each ion-selective electrode is comprised of a membrane which is selectively permeable to the ion or other substance whose activity or concentration is being determined; a reference electrode; and an internal reference material.

Abstract: In an electrode for determining the potassium ion content of a liquid sample to be tested, the electrode including a membrane having incorporated therein an ion selective component, the improvement in which the ion specific component is a compound of the formula: ##STR1## wherein each R.sub.1, R.sub.2, R.sub.3, R.sub.4, R.sub.5, R.sub.6, R.sub.7, and R.sub.8 is hydrogen or an alkyl group containing between one and about 20 carbon atoms, p is 0 or 1, n is one or more, and, each s and r, independently, is 1, 2, or 3, provided that, when p is 1, n is 8 or more.