Abstract: Hybridoma cell lines have been generated which produce and secrete monoclonal antibodies which selectively bind to the ceftiofur. These hybridomas may be obtained by using as an immunization agent or immunogen, desfuroyl ceftiofur which has been conjugated to an immunogenic carrier. Ceftiofur in biological samples may be detected and quantified by contacting the sample with the antibodies to form a ceftiofur/antibody immunocomplex when ceftiofur is present, which immunocomplex may then be detected. The monoclonal antibodies may also be incorporated into kits for the detection and quantification of ceftiofur.

Abstract: Immortal avian T lymphocyte cell lines which produce and secrete immune lymphokines are disclosed. These cell lines may be produced from T cells recovered from fowl which have been hyperimmunized in vivo. The activated T cells are first exposed in vitro to a mitogen effective for secondary stimulation thereof, and then virally transformed to produce an immortal cell line. When cultured in vitro, the cell lines produce and secrete immune lymphokines which may be administered to fowl to increase their resistance to infections.

Abstract: A method of combatting microbial infections, including Escherichia coli and Salmonella infections, in immature birds by in ovo administration of an immune lymphokine (ILK) containing preparation in an amount effective to combat microbial infections in immature birds. A method of combatting microbial infections in immature birds by upregulating the production of endogenous Interleukin-8 (IL-8) in the bird. A method of combatting microbial infections in immature birds by in ovo administration of immune lymphokine (ILK).

Abstract: Immortal avian T lymphocyte cell lines which produce and secrete immune lymphokines are disclosed. These cell lines may be produced from T cells recovered from fowl which have been hyperimmunized in vivo. The activated T cells are first exposed in vitro to a mitogen effective for secondary stimulation thereof, and then virally transformed to produce an immortal cell line. When cultured in vitro, the cell lines produce and secrete immune lymphokines which may be administered to fowl to increase their resistance to infections.

Abstract: Hybridoma cell lines have been produced which secrete monoclonal antibodies that bind hygromycin B and are effective to detect hygromycin B levels as low as about 0.7 ppm. These monoclonal antibodies may be used for the detection and quantitative determination of trace amounts of hygromycin B in samples, especially in animal tissue and feed material.

Abstract: A defined probiotic or composition of anaerobic bacteria effective for controlling or inhibiting Salmonella colonization of fowl. The probiotic includes populations or cultures of substantially biologically pure bacteria, which bacteria include:(a) at least one Lactobacillus species;(b) one or both of:Lactococcus lactis, andCitrobacter freundii; and(c) at least one of:one or more Enterococcus species,one or more Bifidobacterium species,one or more Propionibacterium species, andone or more Escherichia species.In use, the probiotic is administered to the subject fowl in an amount effective for inhibiting Salmonella colonization thereof. The invention also relates to a novel method for isolating probiotics which are effective for controlling or inhibiting Salmonella colonization of fowl, from fecal droppings or cecal contents of adult fowl. The droppings or cecal contents are combined with a culture medium and incubated without dilution (i.e. batch culture) under anaerobic conditions.

Abstract: Hybridoma cell lines have been generated which produce and secrete monoclonal antibodies which selectively bind bovine haptoglobin, either in its native state or bound to hemoglobin and/or albumin. These hybridomas may be obtained by using as an immunization agent or immunogen, bovine haptoglobin that has been complexed to hemoglobin and optionally albumin. Total bovine haptoglobin, including both native and bound to hemoglobin and/or albumin, present in biological samples may be detected and quantified by contacting the sample with the antibodies to form a bovine haptoglobin/antibody immunocomplex when the bovine haptoglobin is present, which immunocomplex may then be detected. The monoclonal antibodies may also be incorporated into kits for the detection and quantification of bovine haptoglobin.

Abstract: A defined probiotic or composition of anaerobic bacteria effective for controlling or inhibiting Salmonella colonization of fowl. The probiotic includes populations or cultures of 29 substantially biologically pure bacteria. In use, the probiotic is administered to the subject fowl in an amount effective for increasing resistance to Salmonella colonization thereof.The invention also relates to a novel method for isolating probiotics which are effective for controlling or inhibiting Salmonella colonization of domestic animals, from fecal droppings, cecal contents or large intestines of the adult animals. The droppings, cecal contents or large intestine are combined with a culture medium and incubated without dilution (i.e. batch culture) under anaerobic conditions. Following this preliminary incubation, the resultant culture is subjected to continuous flow conditions until a steady state is achieved, after which time the steady state culture may be recovered for use as a probiotic.

Abstract: Hybridoma cell lines have been produced which produce and secrete monoclonal antibodies which bind salinomycin and are effective to detect salinomycin levels as low as about 0.174 to about 0.793 ng. These monoclonal antibodies may be used for the detection and quantitative determination of very low levels of salinomycin in samples, especially in animal tissue and feed material.

Abstract: A defined probiotic or composition of anaerobic bacteria effective for controlling or inhibiting Salmonella colonization of fowl. The probiotic includes populations or cultures of substantially biologically pure bacteria, which bacteria include:(a) at least one Lactobacillus species;(b) one or both of:Lactococcus lactis, andCitrobacter freundii; and(c) at least one of:one or more Enterococcus species,one or more Bifidobacterium species,one or more Propionibacterium species, andone or more Escherichia species.In use, the probiotic is administered to the subject fowl in an amount effective for inhibiting Salmonella colonization thereof.The invention also relates to a novel method for isolating probiotics which are effective for controlling or inhibiting Salmonella colonization of fowl, from fecal droppings or cecal contents of adult fowl. The droppings or cecal contents are combined with a culture medium and incubated without dilution (i.e. batch culture) under anaerobic conditions.

Abstract: Salmonella colonization of fowl may be effectively controlled or inhibited using a defined composition of anaerobic bacteria (i.e., probiotic). the probiotic includes a first culture of substantially pure anaerobic bacteria effective for fermenting lactic acid to produce volatile organic acids including acetic, propionic and/or butyric acids. A second culture of at least one anaerobic bacteria effective for fermenting carbonhydrates to produce lactic acid is also employed in combination with the above-mentioned first cuture. Suitable organisms for use in this first culture include bacteria of the genera Veillonella, Megasphaera, and mixtures thereof. These first and second cultures are administered to the subject fowl in amounts effective for inhibiting Salmonella colonization thereof. In one embodiment, the above-mentioned probiotic may be combined with a conventional feed, providing a novel feed product which may be orally ingested by the fowl.

Abstract: An apparatus for encapsulating additives in resealed erythrocytes using dialysis is disclosed. A specimen holder, provided with vertical rotation means, is immersed in a dialyzing solution. Means are also provided for: agitation, cooling, heating, temperature control and measurement of the dialyzing solution.

Abstract: A process for encapsulating additives in resealed erythrocytes from the blood of certain mammals. The invention provides a method of preparing material in substantial quantities, to be employed as vaccine which, when reinjected into these mammals, provides a systemic protection against bloodsucking insects or other parasites.

Abstract: An apparatus and a process for encapsulating additives in resealed erythrocytes from the blood of certain mammals. The invention provides a means of preparing material in substantial quantities, to be employed as vaccine which, when reinjected into these mammals, provides a systemic protection against blood-sucking insects or other parasites.