Patents by Inventor John R. Melki

John R. Melki has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Isothermal strand displacement amplification using primers containing a non-regular base
    Patent number: 8431347
    Abstract: The invention is directed to a method for isothermal DNA amplification comprising providing to the DNA to be amplified an amplification mix comprising a first primer at least partially complementary to a region of DNA and containing a non-regular base, a second primer at least partially complementary to a region of DNA and containing a non- regular base, a DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises a non-regular base in double-stranded DNA and causes a nick or excises a base in one DNA strand at or near the non-regular base; and amplifying the DNA substantially without thermal cycling.
    Type: Grant
    Filed: May 25, 2006
    Date of Patent: April 30, 2013
    Assignee: Human Genetic Signatures Pty Ltd
    Inventors: Douglas Spencer Millar, John R. Melki, Ailsa Grigg
  • Assay for screening for potential cervical cancer
    Patent number: 8343738
    Abstract: The invention relates to an assay for determining a health state of a subject using a combination of detecting the presence of a virus and detecting the presence of a genomic target or marker indicative of a health state.
    Type: Grant
    Filed: September 14, 2006
    Date of Patent: January 1, 2013
    Assignee: Human Genetic Signatures Pty. Ltd.
    Inventors: Douglas Spencer Millar, John R. Melki, George L. Gabor Miklos
  • BISULPHITE TREATMENT OF RNA
    Publication number: 20100286379
    Abstract: The invention relates to a method for bisulphite treating RNA comprising reacting RNA with a bisulphite reagent at 50-90° C. for 5-180 minutes so as to form treated RNA and recovering the treated RNA.
    Type: Application
    Filed: December 4, 2008
    Publication date: November 11, 2010
    Applicant: HUMAN GENETIC SIGNATURES PTY LTD.
    Inventors: Douglas Spencer Millar, John R. Melki
  • Isothermal Strand Displacement Amplification Using Primers Containing a Non-Regular Base
    Publication number: 20100221785
    Abstract: The invention is directed to a method for isothermal DNA amplification comprising providing to the DNA to be amplified an amplification mix comprising a first primer at least partially complementary to a region of DNA and containing a non-regular base, a second primer at least partially complementary to a region of DNA and containing a non-regular base, a DNA polymerase, an enzyme capable of strand displacement, an enzyme that recognises a non-regular base in double-stranded DNA and causes a nick or excises a base in one DNA strand at or near the non-regular base; and amplifying the DNA substantially without thermal cycling.
    Type: Application
    Filed: May 25, 2006
    Publication date: September 2, 2010
    Applicant: HUMAN GENETIC SIGNATURES PTY LTD
    Inventors: Douglas Spencer Millar, John R. Melki, Geoffrey W. Grigg, Ailsa Grigg
  • ASSAY FOR GENE EXPRESSION
    Publication number: 20100092972
    Abstract: An assay for gene expression comprising treating RNA with an agent such as bisulphate that substantially removes secondary structure of the RNA; and measuring the presence or amount of treated RNA so as to obtain an indication of gene expression. The invention also includes use of oligonucleotide, PNA, LNA or INA probes in the assay.
    Type: Application
    Filed: March 14, 2008
    Publication date: April 15, 2010
    Applicant: HUMAN GENETIC SIGNATURES PTY LTD.
    Inventors: Douglas Spencer Millar, John R. Melki, Geoffrey W. Grigg
  • ASSAY FOR A HEALTH STATE
    Publication number: 20100041013
    Abstract: The invention relates to an assay for determining a health state of a subject using a combination of detecting the presence of a virus and detecting the presence of a genomic target or marker indicative of a health state.
    Type: Application
    Filed: September 14, 2006
    Publication date: February 18, 2010
    Applicant: HUMAN GENETIC SIGNATURES PTY LTD.
    Inventors: Douglas Spencer Millar, John R. Melki, George Gabor L. Miklos