Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Abstract: The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest.

Abstract: Embodiments of the present invention are directed to methods and devices/systems for amplifying genetic material and may include providing a water-in-oil emulsion in a continuous flow. The emulsion may include a plurality of water droplets comprising microreactors. Each of the plurality of microreactors may include a single bead capable of capturing a nucleic acid template, a single species nucleic acid template and sufficient reagents to amplify the copy number of the nucleic acid template. The method also includes flowing the emulsion across a first temperature zone and a second lower temperature zone to thermally process the microreactors to amplify the nucleic acid template by polymerase chain reaction.

Abstract: Embodiments of the present invention are directed to methods and devices/systems for amplifying genetic material and may include providing a water-in-oil emulsion in a continuous flow. The emulsion may include a plurality of water droplets comprising microreactors. Each of the plurality of microreactors may include a single bead capable of capturing a nucleic acid template, a single species nucleic acid template and sufficient reagents to amplify the copy number of the nucleic acid template. The method also includes flowing the emulsion across a first temperature zone and a second lower temperature zone to thermally process the microreactors to amplify the nucleic acid template by polymerase chain reaction.

Abstract: A connector for connecting segments of one or more pneumatic tubes including a first member, a second member and a sealing element. The first member and second member are configured to interconnect with each other with the sealing element therebetween. The first member and second member each include at least one cooperating nipple which is insertable into a corresponding port formed through the sealing element. An airtight interference fit is formed between each port and the associated parts of the nipples inserted therein. The connector may also include a mechanism which identifies a type of sensor attached to one of the members to a monitor or other host device attached to the other member. Preferably, the connector also includes an alignment mechanism to ensure proper polarity of pluralities of tube segments associated with each of the first and second members. The connector may also include a keying structure for ensuring that only compatible devices are coupled thereby.

Abstract: An airway valve for re-breathing, a ventilator circuit including same and a method of operating the airway valve. The airway valve includes a housing having first and second primary passages extending through the wall thereof and mutually directly communicating within the housing through a spring-biased valve assembly in the form of first and second diaphragms respectively and simultaneously movable in tandem within the housing into and out of contact with first and second valve seats on a valve body with which the first primary passage communicates. The valve assembly also controls diversion of air flow between the first primary passage and the second primary passage into and through an enlarged volume defined by a re-breathing loop external to the housing via first and second diversion passages, which also extend through the wall of the housing.

Abstract: A stackable mushroom tray having a plurality of curved ribs that extend near but do not contact sidewalls of the tray. Open corner receptacles are formed at each corner of the tray to receive a spacer. Support feet are formed within each corner of the tray to receive a top surface of the spacer. Raised corrugations are placed between the curved ribs with a plurality of holes between the raised corrugations. A lip is formed around the perimeter of the sidewalls. The tray is preferably made of a composite material such as a plastic and fibrous material that can be easily molded. The structure and configuration of the tray permits a relatively thin walled tray to be used, yet is extremely rigid and can be stacked easily while maintaining lateral stability. The tray can be easily manufactured, relatively inexpensively, provides additional growing space, and requires virtually no assembly, greatly simplifying mushroom growing.

Abstract: A heat recovery device is disclosed which is adapted for use in the drain conduit of standard shower installations having a hot water line, a cold water line, means for mixing water from both lines to deliver water at a suitable temperature to a shower head, and a drain conduit for disposing of waste water passing through the shower installation, the heat recovery device transferring heat from the waste water to cold water flowing to the cold water line. The heat recovery device has a first conduit connected into the drain conduit of the shower installation, and a second conduit connected into the cold water line leading into the shower installation and either wrapped around or inserted within the first conduit so that the second conduit is in operative heat transfer relationship with warm waste water passing through the first conduit, so that the incoming cold water is preheated by heat recovered from the waste water and transferred to the cold water.

Abstract: A method for controlling a tape motor using a microcontroller for feeding tape in correspondence to printing of an indeterminate length of printing on the tape by a print drum utilizes a sensor for indicating a tape condition which changes in accordance with the engagement of a printing portion of the print drum with the tape. An optical sensor and slotted disk provide signals indicative of the rotation of a motor shaft of the tape motor. At least first and second counters are provided for counting signal pulses from said optical sensor corresponding to the passage of slots during rotation of the motor. From these counts and the indication of printing by the print drum, the micro controller can develop the timing of control signals for controlling the motor in correspondence to the length of printing on the tape.

Abstract: A thermally insulated floating beverage container holding device is disclosed which has an elongate body member having a peripheral side wall and a bottom wall closing the side wall to form a chamber for receiving a typical round bottle or aluminum can beverage container therein. The side wall has an upper portion that is substantially thicker than the remaining portion of the side wall to provide floation for the holding device, and a weight is suitably secured to the bottom of the holding device to proide sufficient stability that the device holds a beverage container upright both when immersed in water so that neither will beverage spill from the container nor will water get into the container to contaminate the beverage, and when the device is placed on a dry surface.

Abstract: Apparatus for opening an envelope having a front panel, a back panel and a closing flap. The apparatus includes: a device for supporting the envelope in a substantially horizontal plane wherein the back panel is situated above the front panel; a device for holding the closing flap below the back panel; an opening horn for separating the back panel from the front panel, the opening horn being pivotable in a vertical plane; and a device for causing the horn to initially contact the closing flap and to pivot the horn into the envelope between the front and back panels, and to raise the horn inside the envelope, whereby the front and back panels are separated.

Abstract: A method for sequencing the exiting of a cut tape segment from a tape roll feeding, cutting and ejecting apparatus of a mailing machine having a tape roll drive and cut tape segment ejection device and in which a cut tape segment from the tape roll is ejected in an opposite direction from the feeding direction of the feeding of tape of the tape roll drive as it feeds the tape for printing includes the steps of feeding the tape in the feeding direction for a first predetermined time period for extending a predetermined length of tape, further feeding the tape in the feeding direction to create a trail edge margin to assure that the tape is not cut shorter than a minimum length, printing on the tape; then feeding the tape in the opposite direction until one of a first and second conditions occurs, the first condition being the sensing of a leading edge of the tape by a sensor and the second condition being feeding the tape in the opposite direction beyond a second predetermined time period, then at times when t

Abstract: A tape roll rewind device includes a rotatable tape spool with tape wound thereon; and a device for 1) storing energy in response to rotation of the tape spool in a first direction, 2) storing a maximum amount of energy regardless of the amount of rotation of the tape spool in the first direction, and 3) utilizing the stored energy to rotate the tape spool in a second direction to rewind tape onto the tape spool upon the ending of rotation of the tape spool in the first direction.