Patents by Inventor John R. Zysk

John R. Zysk has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Nucleic acid encoding the growth hormone releasing hormone receptor
    Patent number: 5612470
    Abstract: Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromotography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH-R of sufficient purity to conduct sequencing of the receptor. Cloning of a gene encoding for polypeptides (protein or fragments thereof) having GHRH-R activity is accomplished using a bacterial host, and the cloned gene is expressed in a mammalian cell line.
    Type: Grant
    Filed: September 18, 1992
    Date of Patent: March 18, 1997
    Assignees: American Cyanamid Company, The University of Virginia Patent Foundation
    Inventors: Michael O. Thorner, Bruce D. Gaylinn, John R. Zysk, Kevin R. Lynch, Jeffrey K. Harrison
  • Nucleic acid encoding the growth hormone releasing hormone receptor
    Patent number: 5591641
    Abstract: Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromotography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH-R of sufficient purity to conduct sequencing of the receptor. Cloning of a gene encoding for polypeptides (protein or fragments thereof) having GHRH-R activity is accomplished using a bacterial host, and the cloned gene is expressed in a mammalian cell line.
    Type: Grant
    Filed: September 15, 1992
    Date of Patent: January 7, 1997
    Assignees: American Cyanamid Company, The University of Virginia Patent Foundation
    Inventors: Michael O. Thorner, Bruce D. Gaylinn, John R. Zysk, Kevin R. Lynch, Jeffrey K. Harrison
  • Nucleic acid molecule encoding the porcine growth hormone receptor
    Patent number: 5583010
    Abstract: Growth hormone releasing hormone (GHRH) receptor binding has been characterized using a unique binding assay utilizing iodinated GHRH probes. Photoaffinity GHRH probes have been constructed which allow for photolabeling and characterization of the receptor. In addition, high affinity biotinylated GHRH analogs have been constructed. Solubilization of GHRH-R/GHRH complexes and extraction of specifically bound GHRH using a mild detergent solution, followed by affinity chromotography, leads to a substantially purified GHRH-R isolate. Electrophoretic treatment of the GHRH-R isolate produces GHRH-R of sufficient purity to conduct sequencing of the receptor. Cloning of a gene encoding for polypeptides (protein or fragments thereof) having GHRH-R activity is accomplished using a bacterial host, and the cloned gene is expressed in a mammalian cell line.
    Type: Grant
    Filed: June 10, 1993
    Date of Patent: December 10, 1996
    Assignees: American Cyanamid Company, The University of Virginia Patent Foundation
    Inventors: William R. Baumbach, John R. Zysk, Michael O. Thorner, Bruce D. Gaylinn, Kevin R. Lynch, Jeffrey K. Harrison
  • Purified active somatostatin receptor
    Patent number: 5331094
    Abstract: The invention relates to a purified somatostatin receptor which is purified at least about 30,000-fold over the receptor which is membrane-bound. The invention also provides pharmaceutical compositions containing the receptor and antibodies to the receptor.
    Type: Grant
    Filed: October 19, 1992
    Date of Patent: July 19, 1994
    Assignee: American Cyanamid Company
    Inventors: Cecil M. Eppler, John R. Zysk, Martin J. Corbett, Hong-Ming Shieh
  • Receptors method for purification of G protein-linked
    Patent number: 5225543
    Abstract: The receptor:ligand complex is applied to a solid phase substrate which binds the complex by specifically binding the ligand. The receptor is dissociated from the substrate bound receptor:ligand complex with an eluant capable of releasing the receptor from the ligand and leaving the ligand bound to the solid phase substrate. GTP containing eluants are effective. The receptor is recovered from the eluate and optionally subjected to further purification.
    Type: Grant
    Filed: March 28, 1991
    Date of Patent: July 6, 1993
    Assignee: American Cyanamid Company
    Inventors: Cecil M. Eppler, Hong-Ming Shieh, John R. Zysk, Martin J. Corbett