Patents by Inventor John Rambosek
John Rambosek has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 6174706Abstract: DNA encoding triol polyketide synthase (TPKS) has been isolated, purified and sequenced. Expression vectors comprising TPKS, cells transformed with the expression vectors, and processes employing the transformed cells are provided.Type: GrantFiled: January 8, 1998Date of Patent: January 16, 2001Assignee: Merck & Co., Inc.Inventors: Victor A. Vinci, Michael J. Conder, Phyllis C. McAda, Christopher D. Reeves, John Rambosek, Charles Ray Davis, Lee E. Hendrickson
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Patent number: 6071713Abstract: A bioprocess for preparing adipoyl-7-ACA comprising the steps: (a) transforming cells of a strain of Penicillium chrysogenum which produces isopenicillin N with an expression vector containing DNA encoding an enzyme, having expandase activity capable of accepting adipoyl 6-APA as a substrate, an enzyme having hydroxylase activity capable of accepting adipoyl-7-ADCA as a substrate and an enzyme having acetyl transferase activity capable of accepting adipoyl 7-ADAC as a substrate; (b) culturing the transformed cells from step a) in a suitable culture medium containing an adipate feedstock, wherein said cells produce adipoyl 6-APA; and (c) culturing the transformed cells producing adipoyl 6-APA of step b) under conditions suitable for expression of said DNA encoding enzyme, thereby producing the end product adipoyl-7-ACA.Type: GrantFiled: June 28, 1999Date of Patent: June 6, 2000Assignee: Gist-Brocades B.V.Inventors: Michael J. Conder, John A. Rambosek, Phyllis C. McAda, Christopher D. Reeves
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Patent number: 6017726Abstract: A process for making 7-aminodeacetyl-cephalosporanic acid (7-ADAC).Type: GrantFiled: April 17, 1997Date of Patent: January 25, 2000Assignee: Gist-Brocades B.V.Inventors: Michael J. Conder, John A. Rambosek, Phyllis C. McAda, Christopher D. Reeves
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Patent number: 5879914Abstract: This invention relates to recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous.Type: GrantFiled: April 14, 1995Date of Patent: March 9, 1999Assignee: Energy BioSystems CorporationInventors: John Rambosek, Chris S. Piddington, Brian R. Kovacevich, Kevin D. Young, Sylvia A. Denome
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Patent number: 5849541Abstract: DNA encoding triol polyketide synthase (TPKS) from Aspergillus terreus has been isolated, purified and sequenced. Expression vectors comprising said DNA, cells transformed with the expression vectors, and processes employing the transformed cells are provided.Type: GrantFiled: August 23, 1996Date of Patent: December 15, 1998Assignee: Merck & Co., Inc.Inventors: Victor A. Vinci, Michael J. Conder, Phyllis C. McAda, Christopher D. Reeves, John Rambosek, Charles Ray Davis, Lee E. Hendrickson
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Patent number: 5834286Abstract: The present invention provides a recombinant combination strain which is capable of over-expressing at least two different genes under two separate promoters in filamentous fungi. The genes encode phytase and pH 2.5 acid phosphatase. Mixtures containing desired ratios of the two enzymes are prepared by recombinant DNA techniques. The enzyme mixtures show a cooperative effect in the degradation of phytic acid and its salts. The preferred ratios of the two enzymes are from about 3:1 to about 16:1.Type: GrantFiled: May 24, 1995Date of Patent: November 10, 1998Assignee: Rohm Enzyme Finland OyInventors: Helena K. M. Nevalainen, Marja T. Paloheimo, Richard B. Fagerstrom, Arja S. K. Miettinen-Oinonen, Marja K. Turunen, John A. Rambosek, Christopher S. Piddington, Christine S. Houston, Michael A. Cantrell
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Patent number: 5830733Abstract: The present invention provides a nucleic acid molecule encoding a phytase. The present invention also provides a nucleic acid molecule encoding a pH 2.5 acid phosphatase. Also provided are vectors, host cells, and a method of overexpressing phytate degrading enzymes.Type: GrantFiled: March 1, 1996Date of Patent: November 3, 1998Assignee: Rohm Enzyme Finland OyInventors: Helena K. M. Nevalainen, Marja T. Paloheimo, Arja S. K. Miettinen-Oinonen, Tuula K. Torkkeli, Michael Cantrell, Christopher S. Piddington, John A. Rambosek, Marja K. Turunen, Richard B. Fagerstrom, Christine S. Houston
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Patent number: 5780292Abstract: A highly efficient overexpression system for phytase and pH 2.5 acid phosphatase in Trichoderma is described. This system results in enzyme compositions that are especially useful in the animal feed industry.Type: GrantFiled: July 31, 1992Date of Patent: July 14, 1998Assignee: Alko Group Ltd.Inventors: Helena K. M. Nevalainen, Marja T. Paloheimo, Aria S. K. Miettinen-Oinonen, Tuula K. Torkkeli, Michael Cantrell, Cristopher S. Piddington, John A. Rambosek, Marja K. Turunen, Richard B. Fagerstrom
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Patent number: 5744350Abstract: DNA encoding triol polyketide synthase (TPKS) has been isolated, purified and sequenced. Expression vectors comprising TPKS, cells transformed with the expression vectors, and processes employing the transformed cells are provided.Type: GrantFiled: May 25, 1995Date of Patent: April 28, 1998Assignee: Merck & Co., Inc.Inventors: Victor A. Vinci, Michael J. Conder, Phyllis C. McAda, Christopher D. Reeves, John Rambosek, Charles Ray Davis, Lee E. Hendrickson
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Patent number: 5629171Abstract: Important intermediates for preparing cephalosporin antibiotics, 7-amino-cephalosporanic acid (7-ACA) and 7-aminodeacetylcephalosporanic acid (7-ADAC), are prepared by a novel bioprocess in which a transformed Penicillium chrysogenum strain is cultured in the presence of an adipate feedstock to produce adipoyl-6-APA (6-amino penicillanic acid); followed by the in situ expression of the following genes with which the P. chrysogenum has been transformed:1) an expandase gene, whose expression product converts the adipoyl-6-APA by ring expansion to adipoyl-7-ADCA;2) an hydroxylase gene whose expression product converts the 3-methyl side chain of adipoyl-7-ADCA to 3-hydroxymethyl, to give the first product, 7-aminodeacetylcephalosporanic acid (7-ADAC); and3) an acetyltransferase gene whose expression product converts the 3-hydroxymethyl side chain to the 3-acetyloxymethyl side chain of 7-ACA. The final product, 7-ACA, is then prepared by cleavage of the adipoyl side chain using an adipoyl acylase.Type: GrantFiled: May 11, 1995Date of Patent: May 13, 1997Assignee: Gist-Brocades B.V.Inventors: Michael J. Conder, John A. Rambosek, Phyllis C. McAda, Christopher D. Reeves
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Patent number: 5578478Abstract: This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous.Type: GrantFiled: April 14, 1995Date of Patent: November 26, 1996Assignee: Energy BioSystems CorporationInventors: John Rambosek, Chris S. Piddington, Brian R. Kovacevich, Kevin D. Young, Sylvia A. Denome
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Patent number: 5559005Abstract: Important intermediates for preparing cephalosporin antibiotics, 7-amino-cephalosporanic acid (7-ACA) and 7-aminodeacetylcephalosporanic acid (7-ADAC), are prepared by a novel bioprocess in which a transformed Penicillium chrysogenum strain is cultured in the presence of an adipate feedstock to produce adipoyl-6-APA (6-amino penicillanic acid); followed by the in situ expression of the following genes with which the P. chrysogenum has been transformed:1) an expandase gene, e.g., from Cephalosporium acremonium, whose expression product converts the adipoyl-6-APA by ring expansion to adipoyl-7-ADCA;2) an hydroxylase gene whose expression product converts the 3-methyl side chain of adipoyl-7-ADCA to 3-hydroxymethyl, to give the first product, 7-aminodeacetylcephalosporanic acid (7-ADAC); and3) an acetyltransferase gene whose expression product converts the 3-hydroxymethyl side chain to the 3-acetyloxymethyl side chain of 7-ACA.Type: GrantFiled: May 27, 1994Date of Patent: September 24, 1996Assignee: Merck & Co., Inc.Inventors: Michael J. Conder, Phyllis C. McAda, John A. Rambosek, Christopher D. Reeves
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Patent number: 5356801Abstract: This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous.Type: GrantFiled: July 9, 1993Date of Patent: October 18, 1994Assignee: Energy BioSystems CorporationInventors: John Rambosek, Chris S. Piddington, Brian R. Kovacevich, Kevin D. Young, Sylvia A. Denome
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Patent number: 5318896Abstract: An important intermediate for preparing cephalosporin antibiotics, 7-aminodesacetoxy cephalosporanic acid (7-ADCA), is prepared by a novel bioprocess in which a transformed Penicillium chrysogenum strain is cultured in the presence of an adipate feedstock to produce adipoyl-6-APA (6-amino penicillanic acid); and the in situ expression of an expandase gene, e.g., from Streptomyces clavuligerus, with which the P. chrysogenum has been transformed, converts the adipoly-6-APA by ring expansion to adipoyl-7-ADCA. The final product, 7-ADCA, is then prepared by cleavage of the adipoyl side chain using an adipoyl acylase. The entire synthesis, accordingly, is carried out using bioprocesses, and is efficient and economical.Type: GrantFiled: August 28, 1992Date of Patent: June 7, 1994Assignee: Merck & Co., Inc.Inventors: Michael J. Conder, Phyllis C. McAda, John A. Rambosek
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Patent number: 5229274Abstract: A process for the one-step conversion of cephalosporin C and derivatives thereof to the corresponding 7-aminocephalosporanic acid and derivatives comprising treating said cephalosporin C and derivatives with a cephalosporin C amidase enzyme of a recited sequence, the DNA encoding said enzyme, and expression thereof in a suitable host, e.g., Bacillus species under the control of a suitable promoter.Type: GrantFiled: January 13, 1992Date of Patent: July 20, 1993Assignee: Merck & Co., Inc.Inventors: Mark S. Crawford, David B. Finkelstein, John A. Rambosek
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Patent number: 5104800Abstract: A process for the one-step conversion of cephalosporin C and derivatives thereof to the corresponding 7-aminocephalosporanic acid and derivatives comprising treating said cephalosporin C and derivatives with a cephalosporin C amidase enzyme of a recited sequence, the DNA encoding said enzyme, and expression thereof in a suitable host, e.g., Bacillus species under the control of a suitable promoter.Type: GrantFiled: June 27, 1989Date of Patent: April 14, 1992Assignee: Merck & Co., Inc.Inventors: Mark S. Crawford, David B. Finkelstein, John A. Rambosek