Abstract: The present disclosure relates to the discovery of compositions and methods for therapeutic immunization for SARS-CoV-2 infections and/or disease(s) associated with expression of Glypican-3 (GPC3), including but not limited to cancers such as hepatocellular carcinoma (HCC). Methods of the disclosure include a method of generating virus like vesicles (VLVs), VLVs comprising SARS-CoV-2 antigens from a high titer VLV producing vector, VLVs comprising GPC3 antigens from a high titer VLV producing vector, methods of treating, ameliorating, and/or preventing SARS-COV-2 infection, methods of inducing a memory T and B cell immune response against SARS-CoV-2 infection in a, methods of treating, ameliorating, and/or preventing GPC3 associated disease, and methods of inducing a memory T and B cell immune response against GPC3 in a subject.

Abstract: Railcar inspection systems, methods, and apparatuses are disclosed, including a railcar inspection portal. The railcar inspection portal includes a physical structure positioned around a railroad track, and through which a railcar can travel. The railcar inspection portal can include wheel detection sensors along the railroad track for detecting the presence of a railcar passing over the sensors. The sensors can transmit signals, corresponding to railcars passing over the sensors, to computing devices for determining railcar speeds. The railcar inspection portal can include imaging devices configured to capture images and readings of railcars passing through the inspection portal. Based on a determined speed corresponding to a passing railcar, the computing devices can control the imaging devices to capture specific areas or components of the passing railcar, or individual cars thereon.

Abstract: Methods, systems, and devices for treating osteochondral defects (OCDs) are disclosed. The disclosed methods and systems include collecting joint surface data using image-free methods, generating a three-dimensional (3D) healthy bone model based on the joint surface data and a database of healthy bone anatomies, defining the OCD boundary on the joint, generating a 3D implant model based on the 3D healthy bone model and the OCD boundary, manufacturing an implant based on the 3D implant model, generating an implantation plan, the resected cavity on the joint. The implant includes a 3D-printed titanium alloy substrate having a first and second porous layer separated by a nonporous layer. A polymer material is over-molded onto the second porous layer and treated to exhibit properties that mimic cartilage, while the first porous layer allows the implant to fuse to patient bone.

Abstract: Railcar inspection systems, methods, and apparatuses are disclosed, including a railcar inspection portal. The railcar inspection portal includes a physical structure positioned around a railroad track, and through which a railcar can travel. The railcar inspection portal can include wheel detection sensors along the railroad track for detecting the presence of a railcar passing over the sensors. The sensors can transmit signals, corresponding to railcars passing over the sensors, to computing devices for determining railcar speeds. The railcar inspection portal can include imaging devices configured to capture images and readings of railcars passing through the inspection portal. Based on a determined speed corresponding to a passing railcar, the computing devices can control the imaging devices to capture specific areas or components of the passing railcar, or individual cars thereon.

Abstract: Disclosed are compositions and kits useful for bone repair and reconstruction of bone defects such as bone voids and fractures. The compositions can include two reactive components that when mixed together form a polythiourethane that cures to a hard solid. Also disclosed are methods of making and using the compositions.

Abstract: Methods and systems for treating osteochondral defects (OCDs) are disclosed. The methods include collecting surface data of a joint using image-free methods, generating a three-dimensional (3D) healthy bone model based on the surface data and a database of healthy bone anatomies, defining a boundary of the OCD on the joint, and generating a 3D implant model based on the 3D healthy bone model and the boundary. The method may also include manufacturing an implant based on the 3D implant model, generating an implantation plan, resecting the joint according to the implantation plan, and placing the implant into the resected cavity. The 3D implant model may include a first and second porous layer separated by a nonporous layer. A polymer material is overmolded onto the second porous layer and treated to exhibit properties that mimic cartilage, while the first porous layer allows the implant to fuse to patient bone.

Abstract: Railcar inspection systems, methods, and apparatuses are disclosed, including a railcar inspection portal. The railcar inspection portal includes a physical structure positioned around a railroad track, and through which a railcar can travel. The railcar inspection portal can include wheel detection sensors along the railroad track for detecting the presence of a railcar passing over the sensors. The sensors can transmit signals, corresponding to railcars passing over the sensors, to computing devices for determining railcar speeds. The railcar inspection portal can include imaging devices configured to capture images and readings of railcars passing through the inspection portal. Based on a determined speed corresponding to a passing railcar, the computing devices can control the imaging devices to capture specific areas or components of the passing railcar, or individual cars thereon.

Abstract: Railcar inspection systems, methods, and apparatuses are disclosed, including a railcar inspection portal. The railcar inspection portal includes a physical structure positioned around a railroad track, and through which a railcar can travel. The railcar inspection portal can include wheel detection sensors along the railroad track for detecting the presence of a railcar passing over the sensors. The sensors can transmit signals, corresponding to railcars passing over the sensors, to computing devices for determining railcar speeds. The railcar inspection portal can include imaging devices configured to capture images and readings of railcars passing through the inspection portal. Based on a determined speed corresponding to a passing railcar, the computing devices can control the imaging devices to capture specific areas or components of the passing railcar, or individual cars thereon.

Abstract: An orthopaedic implant comprising a titanium substrate having silver deposited thereon, wherein the silver is operable to be eluted at a rate of at least 0.25 ?g/cm2 24 h?1, for at least 14 consecutive days, in use. The invention also extends to a method of producing an orthopaedic implant and use of the same.

Abstract: A method of incorporating silver and/or copper into a biomedical implant includes: providing an implant having an outer surface; depositing silver and/or copper onto the outer surface of the implant; diffusing the silver and/or copper into a subsurface zone adjacent the outer surface; and oxidizing or anodizing the implant after the diffusion step to form an oxidized or anodized layer that contains at least some amount of elemental silver, elemental copper or silver or copper ions or compounds.

Abstract: Methods, systems, and devices for treating osteochondral defects (OCDs) are disclosed. The disclosed methods and systems include collecting joint surface data using image-free methods, generating a three-dimensional (3D) healthy bone model based on the joint surface data and a database of healthy bone anatomies, defining the OCD boundary on the joint, generating a 3D implant model based on the 3D healthy bone model and the OCD boundary, manufacturing an implant based on the 3D implant model, generating an implantation plan, the resected cavity on the joint. The implant includes a 3D-printed titanium alloy substrate having a first and second porous layer separated by a nonporous layer. A polymer material is over-molded onto the second porous layer and treated to exhibit properties that mimic cartilage, while the first porous layer allows the implant to fuse to patient bone.

Abstract: Methods and systems for treating osteochondral defects (OCDs) are disclosed. The methods include collecting surface data of a joint using image-free methods, generating a three-dimensional (3D) healthy bone model based on the surface data and a database of healthy bone anatomies, defining a boundary of the OCD on the joint, and generating a 3D implant model based on the 3D healthy bone model and the boundary. The method may also include manufacturing an implant based on the 3D implant model, generating an implantation plan, resecting the joint according to the implantation plan, and placing the implant into the resected cavity. The 3D implant model may include a first and second porous layer separated by a nonporous layer. A polymer material is overmolded onto the second porous layer and treated to exhibit properties that mimic cartilage, while the first porous layer allows the implant to fuse to patient bone.

Abstract: Methods, systems, and devices for the treatment of osteochondral defects (OCDs) are disclosed. The disclosed method and systems include collecting surface data of a joint using image-free methods, generating a three-dimensional (3D) healthy bone model based on the surface data of the joint and a database of healthy bone anatomies, defining of the boundary of the OCD on the joint, generating a 3D implant model based on the 3D healthy bone model and the boundary of the OCD on the joint, manufacturing an implant based on the 3D implant model, generating an implantation plan, resecting the joint according to the implantation plan, and placing the implant into the resected cavity on the joint. The disclosed devices include two or more distinct segments with distinct structures optimized for bone or cartilage growth and configured to receive distinct injectable biologic enhancement for targeted bone or cartilage growth.

Abstract: Methods, systems, and devices for the treatment of osteochondral defects (OCDs) are disclosed. The disclosed method and systems include collecting surface data of a joint using image-free methods, generating a three-dimensional (3D) healthy bone model based on the surface data of the joint and a database of healthy bone anatomies, defining of the boundary of the OCD on the joint, generating a 3D implant model based on the 3D healthy bone model and the boundary of the OCD on the joint, manufacturing an implant based on the 3D implant model, generating an implantation plan, resecting the joint according to the implantation plan, and placing the implant into the resected cavity on the joint. The disclosed devices include two or more distinct segments with distinct structures optimized for bone or cartilage growth and configured to receive distinct injectable biologic enhancement for targeted bone or cartilage growth.

Abstract: An orthopaedic implant comprising a titanium substrate having silver deposited thereon, wherein the silver is operable to be eluted at a rate of at least 0.25 ?g/cm2 24 h-1, for at least 14 consecutive days, in use. The invention also extends to a method of producing an orthopaedic implant and use of the same.

Abstract: Orthopedic implants having antimicrobial properties and methods of producing such orthopedic implants are provided. In various embodiments, the present disclosure pertains to orthopedic implants that comprise a metal substrate and a surface coating comprising chlorhexidine or a salt of chlorhexidine on a surface of the metal substrate. In various embodiments, the present disclosure also pertains to methods of making orthopedic implants that comprise: (a) pre-treating a metal substrate to form a pretreated metal substrate and (b) applying a coating comprising chlorhexidine or a salt of chlorhexidine on a surface of the pretreated metal substrate.

Abstract: An orthopaedic implant comprising a titanium substrate having silver deposited thereon, wherein the silver is operable to be eluted at a rate of at least 0.25 ?g/cm2 24h-1, for at least 14 consecutive days, in use. The invention also extends to a method of producing an orthopaedic implant and use of the same.

Abstract: A multi-layer, fiber-reinforced composite orthopaedic fixation device having a design selected based on a desired characteristic of the orthopaedic fixation device. The design may be selected according to a model of the device, the model defining design constraints, and the design may comprise a pattern of the fiber angle for each layer. The selection of a design may be analyzed using finite element analysis to determine whether the design will comprise the desired characteristic.

Abstract: Devices, systems, and methods for applying a bioactive coating to an exterior surface of an implant are disclosed. In some embodiments, the bioactive coating may be applied to the surfaces of the implant within the operating room at the time of implantation. In one embodiment, the implant may be a temporary spacer used to temporary replace an implant in a patient suffering from an infection. The temporary spacer being, for example, an antibacterial material for fighting the infection. In some embodiments, the method includes providing a mold of the implant, and providing the bioactive coating within the mold. The method may further include inserting the implant into the mold so that the exterior surface of the implant contacts the bioactive coating, and then removing the implant from the mold.

Abstract: Devices, systems, and methods for applying a bioactive coating to an exterior surface of an implant are disclosed. In some embodiments, the bioactive coating may be applied to the surfaces of the implant within the operating room at the time of implantation. In one embodiment, the implant may be a temporary spacer used to temporary replace an implant in a patient suffering from an infection. The temporary spacer being, for example, an antibacterial material for fighting the infection. In some embodiments, the method includes providing a mold of the implant, and providing the bioactive coating within the mold. The method may further include inserting the implant into the mold so that the exterior surface of the implant contacts the bioactive coating, and then removing the implant from the mold.