Abstract: Compositions that are plants, seeds or crops that have a combination of defective BADC genes and genes for making hydroxy fatty acids produced normal levels of oil containing specialty fatty acids, and exhibited an increases in total oil accumulation, increase in absolute hydroxy (specialized) fatty acid accumulation per seed and/or per plant and/or per unit land area. Defective BADC genes and genes for synthesizing hydroxy fatty acids are combined to produce specialized fatty acids without substantially slowing production of endogenous fatty acids. Methods are also described for increasing production of unusual fatty acids and increasing total fatty acid levels in plants by a mechanism involving combining defective BADC genes and genes for making hydroxy fatty acids to produce steady or increased levels of oil containing the increased specialty products as described herein.

Abstract: The invention provides mutant or variant oleosin polypeptides having one or more amino acid substitutions, particularly one or more arginine substitution for lysine, and having one or more amino acid deletions. The mutant oleosin polypeptides provide for higher triacylglycerol compared to wild type oleosin, including when the mutant oleosin is expressed in plants. Also provided are polynucleotides encoding the mutant oleosin(s), constructs and host cells comprising the polynucleotides, methods for producing oil bodies comprising the mutant oleosin(s) and for producing oil in host cells and plants. The invention also relates to plants, particularly transgenic or recombinantly engineered plants, expressing one or more of the mutant oleosin polypeptides, as well as seeds and oil bodies derived from the plants.

Abstract: The invention provides wild type stearoyl-ACP type desaturase, and its mutants, particularly T117R and D280K, for converting oleoyl-ACP, the normal product of the stearoyl-ACP desaturase, to a vicinal diol, erythro 9, 10 dihydroxy stearate. The invention provides mutant or variant stearoyl-ACP type desaturase polypeptides having one or more amino acid substitutions, particularly one or more substitution at amino acid 117 and/or amino acid 280, of the plastid enzyme polypeptide. The mutant polypeptides provide for higher vicinal diol, particularly 9, 10 dihydroxy stearate, compared to wild type stearoyl-acyl carrier protein (ACP) desaturase, including when the mutant stearoyl-ACP type desaturase is expressed in host cells. Also provided are polynucleotides encoding the mutant stearoyl-ACP type desaturase, constructs and host cells comprising the polynucleotides, methods for producing a vicinal diol, erythro 9, 10 dihydroxy stearate, in host cells.

Abstract: Heterologous expression of E. coli cyclopropane synthase in genotypic and phenotypic fad2fae1 plants facilitates accumulation of cyclopropane fatty acids. Co-expression of Sterculia foetida transferases, including lysophosphatidic acid acyltransferase, diacylglycerol acyltransferase (DGAT), and Phospholipid Diacyl Glycerol Acyltransferase (PDAT), with E. coli cyclopropane synthase further enhances cyclopropane fatty acid accumulation in fad2fae1 plant seeds.

Abstract: The invention provides combining certain genetic mutations in plants to increase sugar levels in the vegetative tissues of the plants. The specific combinations of mutations yield increased oil production in the vegetative tissues. In one embodiment, a plant comprises two mutations, wherein the two mutations are an adg1 mutation and a suc2 mutation. In one embodiment, a plant comprises three mutations, wherein the three mutations are an adg1 mutation, a suc2 mutation and a sdp1 mutation, or comprises an adg1 mutation, a suc2 mutation and a tt4 mutation. In one embodiment, the plant comprises four mutations, wherein the four mutations are an adg1 mutation, a suc2 mutation, an sdp1 mutation, and a tgd1 mutation, or comprises an adg1 mutation, a suc2 mutation, a tt4 mutation and a tgd1 mutation.

Abstract: Compositions and methods include genetically encoding and expressing a novel ?9-18:0-ACP desaturase in plant cells. In some embodiments, nucleic acid molecules encode the novel ?9-18:0-ACP desaturase. In other embodiments, amino acid sequences have ?9-18:0-ACP desaturase activity. Methods can involve expression of ?9-18:0-ACP desaturase in plant cells, plant materials, and whole plants for the purpose of increasing the amount of unusual fatty acids in whole plants, plant seeds, and plant materials, for example, seeds.

Abstract: The present disclosure provides methods and compositions for genetic transformation of Lemnaceae species.

Abstract: The present invention discloses a novel gene encoding a transporter protein trigalactosyldiacylglycerol-5 (TGD5), mutations thereof and their use to enhance TAG production and retention in plant vegetative tissue.

Abstract: In the tgd1-1 mutant that displays substantially enhanced TAG synthesis and turnover, disruption of SUGAR-DEPENDENT1 (SDP1) TAG lipase or PEROXISOMAL TRANSPORTER1 (PXA1) severely decreases FA turnover, leading to an increase in leaf TAG content up to 9% of dry weight and total leaf lipid by three-fold. The membrane lipid content and composition of tgd1-1 sdp1-4 and tgd1-1 pxa1-2 double mutants are altered and they are compromised in growth and development and fertility.

Abstract: Compositions and methods include genetically encoding and expressing a novel ?9-18:0-ACP desaturase in plant cells. In some embodiments, nucleic acid molecules encode the novel ?9-18:0-ACP desaturase. In other embodiments, amino acid sequences have ?9-18:0-ACP desaturase activity. Methods can involve expression of ?9-18:0-ACP desaturase in plant cells, plant materials, and whole plants for the purpose of increasing the amount of unusual fatty acids in whole plants, plant seeds, and plant materials, for example, seeds.

Abstract: Heterologous expression of E. coli cyclopropane synthase in genotypic and phenotypic fad2fae1 plants facilitates accumulation of cyclopropane fatty acids. Co-expression of Sterculia foetida transferases, including lysophosphatidic acid acyltransferase, diacylglycerol acyltransferase (DGAT), and Phospholipid Diacyl Glycerol Acyltransferase (PDAT), with E. coli cyclopropane synthase further enhances cyclopropane fatty acid accumulation in fad2fae1 plant seeds.

Abstract: The present disclosure provides methods and compositions for genetic transformation of Lemnaceae species.

Abstract: Compositions and methods include genetically encoding and expressing a novel ?9-18:0-ACP desaturase in plant cells. In some embodiments, nucleic acid molecules encode the novel ?9-18:0-ACP desaturase. In other embodiments, amino acid sequences have ?9-18:0-ACP desaturase activity. Methods can involve expression of ?9-18:0-ACP desaturase in plant cells, plant materials, and whole plants for the purpose of increasing the amount of unusual fatty acids in whole plants, plant seeds, and plant materials, for example, seeds.

Abstract: In the tgd1-1 mutant that displays substantially enhanced TAG synthesis and turnover, disruption of SUGAR-DEPENDENT1 (SDP1) TAG lipase or PEROXISOMAL TRANSPORTER1 (PXA1) severely decreases FA turnover, leading to an increase in leaf TAG content up to 9% of dry weight and total leaf lipid by three-fold. The membrane lipid content and composition of tgd1-1 sdp1-4 and tgd1-1 pxa1-2 double mutants are altered and they are compromised in growth and development and fertility.

Abstract: The present invention discloses a novel gene encoding a transporter protein trigalactosyldiacylglycerol-5 (TGD5), mutations thereof and their use to enhance TAG production and retention in plant vegetative tissue.

Abstract: A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.

Abstract: The present invention is related to compositions and methods for enhanced synthesis of hydrocarbons, particularly, but not limited to, alkanes. The invention, in one embodiment, utilizes the co-expression of a hydrogen peroxide metabolizing enzyme in the presence of an aldehyde decarbonylase enzyme to relieve hydrogen peroxide inhibition of the aldehyde decarbonylase enzyme by hydrogen peroxide. In a preferred embodiment a catalase-aldehyde decarbonylase expression construct and fusion peptide is used. The present invention also relates to microorganisms engineered to express said enzymes and to produce hydrocarbon molecules.

Abstract: A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.

Abstract: Methods and means are provided to increase the oil content of plants, particularly oleaginous plants by preventing feedback inhibition by 18:1-Coenzyme A or 18:1-Acyl Carrier Protein of the acetyl CoA-carboxylase enzyme in cells of these plants in various manners.

Abstract: A nucleotide construct comprising a nucleotide sequence that forms a stem and a loop, wherein the loop comprises a nucleotide sequence that modulates expression of a target, wherein the stem comprises a nucleotide sequence that modulates expression of a target, and wherein the target modulated by the nucleotide sequence in the loop and the target modulated by the nucleotide sequence in the stem may be the same or different. Vectors, methods of regulating target expression, methods of providing a cell, and methods of treating conditions comprising the nucleotide sequence are also disclosed.