Abstract: Methods and systems provided herein address current limitations of bisulfite-based methylation sequencing by improving the quality and accuracy of nucleic acid methylation sequencing and uses thereof for detection of disease. Methods that include minimally-destructive conversion methods for methylation sequencing as well as specialized UMI adapters provide for improved quality of sequencing libraries and sequencing information. Greater accuracy and more complete methylation-state information permits higher quality feature generation for use in machine learning models and classifier generation.

Abstract: The present disclosure provides methods and systems for screening or detecting a colorectal cancer or following colorectal disease progression that may be applied to cell-free nucleic acids such as cell-free DNA. The method may use detection of methylation signals within a single sequencing read in identified genomic regions as input features to train a machine learning model and generate a classifier useful for stratifying populations of individuals. The method may comprise extracting DNA from a cell-free sample obtained from a subject, converting the DNA for methylation sequencing, generating sequencing reads, and detecting colon proliferative cell disorder-associated signals in the sequencing information and training a machine learning model to provide a discriminator capable of distinguishing groups in a subject population such as healthy, cancer or distinguishing disease subtype or stage. The method may be used for, e.g.

Abstract: The present disclosure provides methods and systems for screening or detecting a colorectal cancer or following colorectal disease progression that may be applied to cell-free nucleic acids such as cell-free DNA. The method may use detection of methylation signals within a single sequencing read in identified genomic regions as input features to train a machine learning model and generate a classifier useful for stratifying populations of individuals. The method may comprise extracting DNA from a cell-free sample obtained from a subject, converting the DNA for methylation sequencing, generating sequencing reads, and detecting colon proliferative cell disorder-associated signals in the sequencing information and training a machine learning model to provide a discriminator capable of distinguishing groups in a subject population such as healthy, cancer or distinguishing disease subtype or stage. The method may be used for, e.g.

Abstract: A modular steering console. The modular steering console has a main console portion having a top side and a side. The top side includes holes for receiving bolts. The side of the main console portion has holes for receiving bolts. The modular steering console has one or more brackets having slots in a top side configured to be fastened to the main console top side by bolts through the slots to allow lateral adjustment of the brackets while allowing a side of the brackets and the side of the main console portion to sandwich a side of a boat.

Abstract: A molded boat with a seat drain. The boat includes a molded hull having been molded using a first material to cause the boat to have a front portion and a rear portion. The boat is molded with a bench, having been molded together with the hull using the first material. The bench includes a drain hole formed through, an otherwise solid face of the bench allowing water to flow from the front portion of the boat to the rear portion of the boat, wherein the drain hole formed through the bench is formed by using a tube molded into, and under the bench, the tube comprising a material having a higher melting point than the first material.

Abstract: Methods and systems provided herein address current limitations of bisulfate-based methylation sequencing by improving the quality and accuracy of nucleic acid methylation sequencing and uses thereof for detection of disease. Methods that include minimally-destructive conversion methods for methylation sequencing as well as specialized UMI adapters provide for improved quality of sequencing libraries and sequencing information. Greater accuracy and more complete methylation-state information permits higher quality feature generation for use in machine learning models and classifier generation.

Abstract: A molded boat with a seat drain. The boat includes a molded hull having been molded using a first material to cause the boat to have a front portion and a rear portion. The boat is molded with a bench, having been molded together with the hull using the first material. The bench includes a drain hole formed through, an otherwise solid face of the bench allowing water to flow from the front portion of the boat to the rear portion of the boat, wherein the drain hole formed through the bench is formed by using a tube molded into, and under the bench, the tube comprising a material having a higher melting point than the first material.

Abstract: An electro-hydraulic limited slip differential system for a vehicle includes an axle assembly having an axle housing having an aperture formed therein, and an electro-hydraulic limited slip differential assembly disposed within the axle housing. The limited slip differential assembly includes a differential case, a differential gear assembly disposed in the differential case, a clutch assembly having a clutch pack and a clutch actuator assembly, and a hydraulic fitting disposed at least partially within the aperture. The hydraulic fitting is coupled to a hydraulic port of the clutch actuator assembly.

Abstract: Provided herein are compositions and methods for predicting whether a subject with cancer will be responsive to treatment with an EGFR TKI and for the treatment of cancer in a subject.

Abstract: An article of manufacture including a through hole. The article of manufacture includes a body, wherein the body is a plastic molded body, and a tube molded into the body. The tube has a melting point higher than the plastic molded body.

Abstract: Box constructions suitable for packaging, storing and transporting small objects including flat rectangular objects of any size such as books, CD's, DVD's, and the like. The box construction has an outer member which surrounds an inner member. An object is packaged by attaching an object onto a surface of the inner member; positioning the inner member on the outer member; folding panel of the outer member around the object on the inner member; and attaching the panels of the outer member thus enclosing the object within the box construction.

Abstract: Box constructions suitable for packaging, storing and transporting small objects including flat rectangular objects of any size such as books, CD's, DVD's, and the like. The box construction has an outer member which surrounds an inner member. An object is packaged by attaching an object onto a surface of the inner member; positioning the inner member on the outer member; folding panel of the outer member around the object on the inner member; and attaching the panels of the outer member thus enclosing the object within the box construction.

Abstract: The present invention relates to a method of forming shape-retentive and shape-conforming aggregate wound dressings and biomaterials composed of gel nanoparticles and wound or bodily fluid in which the aggregates are held together by non-covalent bond physical forces such as, without limitation, hydrophobic-hydrophilic interactions and hydrogen bonds. The method comprises introducing a dry powder of gel nanoparticles to a wound site in which the nanoparticles absorb some of the blood or wound exudate and coalesce in situ into the claimed shape-retentive aggregate dressing. The method also comprises introducing the dry nanoparticle powder in or on a wet bodily tissue in vivo to form the claimed shape-retentive biomaterial. In addition, the method also comprises incorporating biomedical agents to produce medicated aggregate dressings or biomaterials for a variety of medical applications. This invention also relates to uses of the method of formation of the shape-retentive aggregates of gel nanoparticles.

Abstract: The present invention relates to a method of forming shape-retentive and shape-conforming aggregate wound dressings and biomaterials composed of gel nanoparticles and wound or bodily fluid in which the aggregates are held together by non-covalent bond physical forces such as, without limitation, hydrophobic-hydrophilic interactions and hydrogen bonds. The method comprises introducing a dry powder of gel nanoparticles to a wound site in which the nanoparticles absorb some of the blood or wound exudate and coalesce in situ into the claimed shape-retentive aggregate dressing. The method also comprises introducing the dry nanoparticle powder in or on a wet bodily tissue in vivo to form the claimed shape-retentive biomaterial. In addition, the method also comprises incorporating biomedical agents to produce medicated aggregate dressings or biomaterials for a variety of medical applications. This invention also relates to uses of the method of formation of the shape-retentive aggregates of gel nanoparticles.

Abstract: The present invention relates to a method of forming shape-retentive and shape-conforming aggregate wound dressings and biomaterials composed of gel nanoparticles and wound or bodily fluid in which the aggregates are held together by non-covalent bond physical forces such as, without limitation, hydrophobic-hydrophilic interactions and hydrogen bonds. The method comprises introducing a dry powder of gel nanoparticles to a wound site in which the nanoparticles absorb some of the blood or wound exudate and coalesce in situ into the claimed shape-retentive aggregate dressing. The method also comprises introducing the dry nanoparticle powder in or on a wet bodily tissue in vivo to form the claimed shape-retentive biomaterial. In addition, the method also comprises incorporating biomedical agents to produce medicated aggregate dressings or biomaterials for a variety of medical applications. This invention also relates to uses of the method of formation of the shape-retentive aggregates of gel nanoparticles.

Abstract: The present invention relates to a method of forming shape-retentive aggregates of gel particles in which the aggregates are held together by non-covalent bond physical forces such as, without limitation, hydrophobic-hydrophilic interactions and hydrogen bonds. The method comprises introducing a suspension of gel particles in a polar liquid at a selected concentration, wherein the gel particles have an absolute zeta potential, into a medium in which the absolute zeta potential of the gel particles is decreased, resulting in the gel particles coalescing into the claimed shape-retentive aggregate. This invention also relates to uses of the method of formation of the shape-retentive aggregates of gel particles.

Abstract: The present invention relates to a method of forming shape-retentive aggregates of gel particles in which the aggregates are held together by non-covalent bond physical forces such as, without limitation, hydrophobic-hydrophilic interactions and hydrogen bonds. The method comprises introducing a suspension of gel particles in a polar liquid at a selected concentration, wherein the gel particles have an absolute zeta potential, into a medium in which the absolute zeta potential of the gel particles is decreased, resulting in the gel particles coalescing into the claimed shape-retentive aggregate. This invention also relates to uses of the method of formation of the shape-retentive aggregates of gel particles.

Abstract: Disclosed is a new class of nanostructured polymeric materials comprising polymer gel nanoparticles that are covalently bonded through functional groups on the surfaces of neighboring particles. These nanoparticles may be prepared as suspensions in an aqueous or, non-aqueous environment. These gels have two unique and different structural networks; the primary network comprises crosslinked polymer chains in each individual particle, while the secondary network is a system of crosslinked nanoparticles. Particular polymer gel nanoparticle network compositions disclosed herein may function as carriers for controlled delivery of pharmaceuticals or other chemical agents, gel sensors and other commercial applications.