Abstract: The present disclosure relates generally to compositions, methods, and systems for the characterization of binding characteristics of antigen binding molecules, e.g., antibodies, using single-cell immune profiling methodologies. The compositions, methods and systems described herein permit rapid, high-throughput identification and characterization of antigen binding molecules having desired properties.

Abstract: Provided herein are methods for identification of antigen binding molecules such as antibodies from a sample by exposing the antigen binding molecules to an antigen conjugated to an oligonucleotide.

Abstract: The present disclosure relates generally to compositions, methods, and systems for the characterization of T cell receptors and other antigen-binding molecules having T cell-like receptors, e.g., T cell receptor-like antibodies, using single-cell immune profiling methodologies. The compositions, methods and systems described herein permit rapid, high-throughput identification and characterization of T cell, and/or T cell-like, receptors having desired properties.

Abstract: Provided herein, inter alia, are methods and compositions for inhibiting DNase II-mediated degradation of reporter nucleic acid molecules coupled to antigens of interest, and thus improving sensitivity of single-cell immune profiling workflows,

Abstract: The present disclosure generally relates to compositions (e.g., nucleic acid constructs, vectors, plasmids, engineered cells, etc.) and methods using the same for selecting and/or making an antigen-binding molecule of interest (e.g., an immune receptor, an antibody or functional fragment thereof) with improved affinity to a target antigen.

Abstract: The present disclosure generally relates to methods and systems for obtaining epitope specify of candidate antibodies against one or more known antibodies. Further, the present disclosure also provides systems and methods for epitope binning.

Abstract: The present disclosure generally relates to methods and systems for engineering antibodies, and antigen-binding fragments thereof, to have altered characteristics. The present disclosure also provides a high-throughput method useful for identifying the engineered antibodies, or antigen-binding fragments thereof, that acquired the altered characteristics having performed the methods. These methods and systems have implications, for example, in the rapid development of biotherapeutics having desired and/or improved, e.g., affinity, specificity and/or activity-related, characteristics.

Abstract: The present invention teaches the implementation for a system of networked heterogenous signal capture and analysis sensor-enabled devices tethered in a cooperative multi-protocol wireless local area network (WLAN) providing an automated safety monitoring and response services during an active shooter situation. The present invention describes a method to leverage the standard sensors on most smartphones into a real-time swarming of localized tracking, monitoring and guidance networked to direct people to identified safe zones in the covered build and public venues. The system utilizes multi-device real-time two-way positioning/ranging with acoustic based source geo-location algorithm to pinpoint danger regions within the coverage area. The response system described in this invention activates automatically upon detection of discharge of any firearm in the protected area without manual intervention.

Abstract: The present disclosure relates generally to the field of immunology, and particularly relates to compositions, methods, and systems for the analysis and generation of antigen-binding molecules produced by immune cells obtained from tumor samples (e.g., antibodies produced by B cells obtained from tumor samples or TCRs produced by T cells obtained from tumor samples), using single-cell immune profiling methodologies, so as to produce recombinant antigen-binding molecules (e.g., antibodies, TCRs) with desired properties.

Abstract: Provided herein are methods and systems for measuring secreted antibodies or other analytes from single cells. The methods disclosed herein include the use of capture agents and reporter agents that bind to secreted antibodies, wherein the capture agent and/or the reporter agent can be analyte-specific and/or barcoded, and beads in partitions (e.g., droplets or wells) to measure secreted antibodies on a single cell basis. Further described herein are methods comprising the use of hydrogel-encapsulated cells (e.g., cell beads) to measure secreted and/or cellular analytes from single cells.

Abstract: Provided herein are methods for identification of antigen binding molecules such as antibodies from a sample by exposing the antigen binding molecules to an antigen conjugated to an oligonucleotide.

Abstract: The present disclosure provides methods, compositions, and systems for selective enzymatic gelation of cells, e.g., immune cells, contained in partitions.

Abstract: Provided herein are methods for enrichment from a library of nucleic acid sequences of interest using primers against identification sequences. The nucleic acid sequences of interest can be subsequently cloned, and the protein products can be analyzed.

Abstract: A method for grouping immune cells within an immune cell receptor sequence dataset, is disclosed. An immune cell receptor sequence dataset is obtained from a sample. The dataset includes a plurality of full-length immune cell receptor sequences. Each full-length immune cell receptor sequence can comprise of at least one heavy chain region sequence and one light chain region sequence. Each immune cell receptor sequence is associated with an individual immune cell in the sample. The immune cell receptor sequences associated with a first immune cell and a second immune cell from the sample is compared using a comparison protocol. The first immune cell and the second immune cell are identified as members of the same clonotype if one or more immune cell receptor sequence comparison criteria is met.

Abstract: A method for visualizing multi-antigen binding capabilities of a set of clonotype groups. Clonotype data that identifies a clonotype group derived from an immune cell sequence dataset (e.g., single cell or spatial dataset) is obtained. A set of interactions for the clonotype group is identified. An interaction in the set of interactions is between a set of cells in the clonotype group and a plurality of antigens in which each cell of the set of cells binds to the plurality of antigens. A binding diagram is generated for the clonotype group based on the set of interactions that has been identified. The binding diagram includes a set of interaction representations that visually represents the set of interactions for the clonotype group. An interaction representation in the set of interaction representations visually relates the plurality of antigens and visually indicates a number of cells in the set of cells that bind to the plurality of antigens.

Abstract: A system for receiving, transferring, and storing solar thermal energy. The system includes a concentrating solar energy collector, a transfer conduit, a thermal storage material, and an insulated container. The insulated container contains the thermal storage material, and the transfer conduit is configured to transfer solar energy collected by the solar energy collector to the thermal storage material through a wall of the insulated container.

Abstract: Provided herein are methods and systems for measuring secreted cytokines or other analytes from single cells. The methods disclosed herein include the use of analyte-specific and/or barcoded binding agents (e.g., antibodies) and beads in partitions (e.g., droplets or wells) to measure such analytes on a single cell basis. Further described herein are methods comprising the use of hydrogel-encapsulated cells (e.g., cell beads) to measure secreted and/or cellular analytes from single cells.

Abstract: The present disclosure relates in some aspects to methods and compositions for assessing the target binding capacity of a binding molecule, such as an antigen binding molecule, e.g., a monoclonal antibody. In some embodiments, provided herein is a method of analyzing binding of a barcode-labeled antibody to a target antigen expressed by a cell, wherein cells expressing the candidate antigens are partitioned and wherein detection of the barcoded nucleic acid molecule is indicative of the binding between the antibody or antigen-binding fragment thereof and the candidate antigen or epitope of the cell.

Abstract: A computer-implemented system and method for obtaining product related information. Information related to a product obtained from a plurality of different sources is transformed into processed product data with a plurality of levels. Callouts and contexts are identified in the processed product data. A product-to-chemical continuum is generated by creating callout-context pathway segments between the plurality of levels of the processed product data based on the callouts and contexts identified. A query request for product information is transformed into a set of context search parameters, which is used to traverse the product-to-chemical continuum through the callout-context pathway segments that span the plurality of levels. The product information that matches the set of context search parameters is extracted from the product-to-chemical continuum. The callout-context pathway segments reduce processing resources and time needed to obtain the product information.

Abstract: A system for receiving, transferring, and storing solar thermal energy. The system includes a concentrating solar energy collector, a transfer conduit, a thermal storage material, and an insulated container. The insulated container contains the thermal storage material, and the transfer conduit is configured to transfer solar energy collected by the solar energy collector to the thermal storage material through a wall of the insulated container.