Abstract: An electrochemical cell for converting metal salt or metal oxide to metal comprises: a) a mixture comprising an electrolyte and metal salt or metal oxide; b) an anode submerged in the mixture; c) a cathode partially submerged in the mixture and moveable along a closed loop path; and d) a harvester disposed at an exposed portion of the cathode outside of the mixture, wherein an electrical charge supplied to the electrochemical cell reduces the metal salt or metal oxide to metal at and disposed onto the cathode, and wherein the harvester removes the metal from the exposed portion of the cathode. Methods and systems for converting metal salt or metal oxide to metal are also disclosed including continuous methods and systems.

Abstract: Certain Cas9 molecules are capable of discriminately cleaving nucleic acid based upon its epigenetic pattern. These molecules can be either naturally occurring or engineered. They can be used to diagnose and treat disease.

Abstract: The present disclosure relates to the field of gene genome editing. In particular, it relates to the provision of a CRISPR-Cas class 2 type V prime editing system, including a prime editor, a prime editor complex, a prime editing guide RNA system as well as the means and methods for the modification of a nucleic acid sequence of interest with the prime editing system of the present invention.

Abstract: Provided herein are recombinant antigen receptors, for example chimeric antigen receptors (CARs), that comprise modified cytoplasmic domains that provide improved signalling and thereby provide improved performance and safety. Also provided are polynucleotides encoding the recombinant antigen receptors, vectors comprising the polynucleotides, and engineered immune cells comprising the vectors and/or polynucleotides. The invention further provides methods for engineering immune cells to express the recombinant antigen receptors. Improved recombinant antigen receptor signalling is also provided by co-expressing a first recombinant antigen receptor and a second recombinant antigen receptor or co-expressing a recombinant antigen receptor and a protein involved in transducing the signal from the activated recombinant antigen receptor.

Abstract: A clustered regularly interspaced short palindromic repeat (CRISPR)-associated complex for adaptive antiviral defence (Cascade); the Cascade protein complex comprising at least CRISPR-associated protein subunits Cas7, Cas5 and Cas6 which includes at least one subunit with an additional amino acid sequence possessing nucleic acid or chromatin modifying, visualising, transcription activating or transcription repressing activity. The Cascade complex with additional activity is combined with an RNA molecule to produce a ribonucleoprotein complex. The RNA molecule is selected to have substantial complementarity to a target sequence. Targeted ribonucleoproteins can be used as genetic engineering tools for precise cutting of nucleic acids in homologous recombination, non-homologous end joining, gene modification, gene integration, mutation repair or for their visualisation, transcriptional activation or repression.

Abstract: When a user accesses a seller webpage for an item for which expedited shipping is available, data regarding this access is provided to a separate fulfillment service that is able to deliver the item. A control on the webpage, that may be provided to the seller by the fulfillment service, presents an initial estimated delivery time based on the network address of the user's device and a known location of the item. The user may provide input to the control to access a user account, and a location for the user account may be used to update the estimated delivery time. If the item is purchased, the fulfillment service may determine payment information for the user account and process payment and delivery of the item. If an additional item not fulfilled by the fulfillment service is also purchased, data for this purchase is provided to the seller website for processing.

Abstract: The present application discloses systems, methods, and computer-readable media that utilize computer-implemented models to monitor surplus provider device slots and transmit digital invitations to provider devices to join an online mode by dynamically leveraging previous transportation provider device requests within a threshold rejection time span. For instance, the disclosed systems can identify rejected provider devices that requested to be in an online mode of a transportation matching application within a geographical area. Then the disclosed systems can monitor device activity over computer networks and determine a number of surplus provider device slots corresponding to the geographical region. Furthermore, the disclosed systems can select provider devices (that were rejected within a threshold rejection time span) from the previously rejected requests.

Abstract: The disclosure provides CARs (CARs) that specifically bind to CD70. The disclosure further relates to engineered immune cells comprising such CARs, CAR-encoding nucleic acids, and methods of making such CARs, engineered immune cells, and nucleic acids. The disclosure further relates to therapeutic methods for use of these CARs and engineered immune cells comprising these CARs for the treatment of a condition associated with malignant cells expressing CD70 (e.g., cancer).

Abstract: Nucleotide sequences of interest, which may be as yet unknown sequences, comprised in a biological sample may often be present in diminishingly small amounts, meaning there are difficulties in detecting, sequencing and identifying these sequences. A method of enrichment of the sequences of interest prior to sequencing of a sample overcomes the problem. In such a method a library of nucleic acid guides is generated from a portion of the sample itself, and the guides are used with a guide-dependent endonuclease such as an Argonaute, usually a prokaryotic Argonaute (pAgo) in a reaction which cleaves the nucleic acids recognised by the guides in another portion of the same sample, but which spares the low abundance sequences for which no guides have been generated. In this way a sample enriched for rarer or low abundance sequences is provided and used in subsequent steps of detection of sequences present, including sequencing of the enriched sample.

Abstract: An example method of radiation therapy in a radiation therapy system that includes a gantry with a treatment-delivering X-ray source and an imaging X-ray source mounted thereon is described. The method includes rotating the gantry in a first direction at a first rotational velocity about an open bore and concurrently rotating an annular support structure at a second rotational velocity about the open bore, wherein the second rotational velocity is less than the first rotational velocity. While continuing to rotate the gantry in the first direction about the open bore from a first position to a treatment position, the method also includes generating multiple images of a target volume disposed in the bore using the imaging X-ray source. Upon rotating the gantry to the treatment position, the method includes initiating delivery of a treatment beam to the target volume with the treatment-delivering X-ray source.

Abstract: Methods, systems, and apparatus, including computer programs encoded on a computer storage medium, for generating guidance data to be provided to a target agent interacting with an environment. In one aspect, a method comprises: generating a prompt to be provided to a generative model based at least in part on: (i) one or more target trajectories representing interactions of the target agent with the environment, and (ii) a plurality of reference trajectories representing interactions of each of a plurality of reference agents with the environment, wherein each of the plurality of reference agents differ from the target agent; and generating the guidance data for the target agent using the generative model while the generative model is conditioned on the prompt; and providing the guidance data to the target agent.

Abstract: The invention relates to a protein having at least 50% sequence identity with SEQ ID NO:1, and its use in in vitro transcription methods. The invention further relates to a nucleic acid molecule encoding the protein and to a host cell expressing the protein.

Abstract: The present invention relates to a support device for assisting a user in showering or toileting. The support device comprises a seat comprising a seating surface for supporting a weight of the user; a plurality of legs attached to the seat; a pair of handles configured to attach to substantially opposite side portions of the seat, each handle comprising a pair of end portions for attaching to the seat; and a securing element for securing each handle to the seat; wherein each of the substantially opposite side portions of the seat comprises a plurality of receptacles for snugly fitting the end portions of a respective handle, and wherein the support device is configured such that a portion of the user's weight on the handles of the support device applies a force that has a force component directed into each receptacle and parallel to a central axis of each receptacle.

Abstract: A mounting assembly (100) for mounting a camera (2) at a construction (1) and a related method are disclosed. A mounting part (200) is arranged to be mounted at the construction (1); and an attachment part (300) is arranged to be attached to the camera (2). Mounting plates (221, 222) comprises a respective open slot (231, 232), and a respective rotation through hole (241, 242). Guiding plates (321, 322) comprises a respective guiding element (331, 332) and a respective center through hole (341, 342) that is located to be capable of being aligned with the respective rotation through hole (241, 242) of said each mounting plate (221, 222) when the respective guiding element (331, 332) is located in the respective open slot (231, 232).

Abstract: A filter cleaning unit for a vacuum cleaner is provided, in particular a construction site vacuum cleaner, wherein the vacuum cleaner includes a turbine for generating a suction air flow and a filter for filtering the suction air flow. A suction channel is provided between the turbine and the filter, wherein the suction channel can be divided by a separating element into at least a first suction part channel and a second suction part channel, wherein the filter cleaning unit has at least one valve unit for opening or closing the suction part channels. A vacuum cleaner with such a filter cleaning unit, and a method for cleaning a filter of a vacuum cleaner with a filter cleaning unit is also provided. A controllable filter cleaning can be provided, in which the cleaning effect can be reinforced by the advantageous interaction of the filter cleaning unit and its components.

Abstract: A dispensing apparatus is provided. The apparatus includes multiple dosing stations each having an output opening, multiple fall ducts mounted to a mounting beam of a fall duct conveyor and multiple collectors connected to a collector conveyor. Each fall duct includes a first part and an opposing second part detachably connected to the first part, the second part having multiple inlet openings that align with the output openings of the dosing stations, wherein the first and second parts define a space for solids falling from the dosing stations. The collector conveyor moves the collectors together with the fall ducts moved by the fall duct conveyor, wherein the collectors collect the solids falling from the dosing stations.

Abstract: The disclosure generally pertains to systems and methods for use of a vehicle to conduct a site survey. An example method can include receiving navigation guidance to move a vehicle along a pre-defined path on a property for executing a site survey operation of the property. The site survey operation can include a staking procedure performed by a robotic arm attached to the vehicle, an image capture procedure performed by use of a camera in the vehicle, and/or a subterranean scanning procedure for detecting a buried object. In an example implementation, the staking procedure can include inserting at a first location on the property, a stake having affixed thereon, a barcode label. A barcode scanner can be used to read the barcode label for obtaining information associated with an object present at the first location.

Abstract: Provided herein are constitutively active chimeric cytokine receptors (CACCRs). When present on chimeric antigen receptor (CAR)-bearing immune cells, such CACCRs allow for increased immune cell activation, proliferation, persistence, and/or potency. Also provided are methods of making and using the CACCRs described herein.

Abstract: Provided herein are recombinant antigen receptors, for example chimeric antigen receptors (CARs), that comprise modified cytoplasmic domains that provide improved signalling and thereby provide improved performance and safety. Also provided are polynucleotides encoding the recombinant antigen receptors, vectors comprising the polynucleotides, and engineered immune cells comprising the vectors and/or polynucleotides. The invention further provides methods for engineering immune cells to express the recombinant antigen receptors. Improved recombinant antigen receptor signalling is also provided by co-expressing a first recombinant antigen receptor and a second recombinant antigen receptor or co-expressing a recombinant antigen receptor and a protein involved in transducing the signal from the activated recombinant antigen receptor.

Abstract: This disclosure relates to compounds, pharmaceutical compositions comprising them, and methods of using the compounds and compositions for treating diseases related to glioma-associated oncogene (Gli) expression. More particularly, this disclosure relates to bicyclic compounds and pharmaceutical compositions thereof, methods of inhibiting Gli expression with these compounds, and methods of treating diseases related to Gli expression.