Patents by Inventor John Verruto

John Verruto has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20240052001
    Abstract: Disclosed herein are mutant photosynthetic microorgnaisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.
    Type: Application
    Filed: September 27, 2023
    Publication date: February 15, 2024
    Inventors: Eric R. Moellering, Nicholas Bauman, Randor R. Radakovits, Roberto Spreafico, Fedor Kuzminov, Imad Ajjawi, Saheed Imam, Andrew Schultz, Kathleen Kwok, Moena Aqui, Jennifer Nominati, John Verruto, Shaun Bailey
  • Patent number: 11787843
    Abstract: Disclosed herein are mutant photosynthetic microorganisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.
    Type: Grant
    Filed: February 17, 2021
    Date of Patent: October 17, 2023
    Assignee: Viridos, Inc.
    Inventors: Eric R. Moellering, Nicholas Bauman, Randor R. Radakovits, Roberto Spreafico, Fedor Kuzminov, Imad Ajjawi, Saheed Imam, Andrew Schultz, Kathleen Kwok, Moena Aqui, Jennifer Nominati, John Verruto, Shaun Bailey
  • Patent number: 11434493
    Abstract: The invention provides important tools for the use of Chlorophyte organisms, including regulatory sequences useful for the expression of transgenes in such organisms. Also provided are vectors and expression cassettes containing promoters and/or terminators disclosed herein for expression of transgenes in Chlorophyte organisms. Methods of using these tools are also provided.
    Type: Grant
    Filed: February 4, 2021
    Date of Patent: September 6, 2022
    Assignee: Viridos, Inc.
    Inventors: John Verruto, Moena Aqui
  • Patent number: 11339399
    Abstract: The present invention provides cell lines for high efficiency genome editing using cas/CRISPR systems, methods of generating such cells lines, and methods of generating mutations in the genome of an organism using such cell lines.
    Type: Grant
    Filed: December 31, 2015
    Date of Patent: May 24, 2022
    Assignee: Viridos, Inc.
    Inventors: John Verruto, Eric Moellering
  • Publication number: 20220025390
    Abstract: The present application provides novel algal regulatory elements including inducible nitrate/nitrite promoter sequences and terminator sequences. The application further discloses DNA constructs comprising these novel regulatory elements, and recombinant microorganisms comprising these regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. Methods disclosed in the present application are suited for inducible expressions of genes, such as a transgene or a native gene in algal species.
    Type: Application
    Filed: October 12, 2021
    Publication date: January 27, 2022
    Inventors: John Verruto, Jessica Weir
  • Patent number: 11162106
    Abstract: The present application provides novel algal regulatory elements including inducible nitrate/nitrite promoter sequences and terminator sequences. The application further discloses DNA constructs comprising these novel regulatory elements, and recombinant microorganisms comprising these regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. Methods disclosed in the present application are suited for inducible expressions of genes, such as a transgene or a native gene in algal species.
    Type: Grant
    Filed: December 18, 2019
    Date of Patent: November 2, 2021
    Assignee: Synthetic Genomics, Inc.
    Inventors: John Verruto, Jessica Weir
  • Patent number: 11124798
    Abstract: The present invention provides mutant microorganisms having attenuated expression of a gene encoding a polypeptide that includes a TPR domain, wherein the mutant microorganisms have higher lipid productivity and/or exhibit increased partitioning of carbon to lipid as compared to wild type microorganisms from which they are derived. Also provided are methods of producing lipids using the mutant microorganisms, guide RNAs, and nucleic acid constructs used for producing mutant microorganisms.
    Type: Grant
    Filed: December 7, 2018
    Date of Patent: September 21, 2021
    Assignee: Synthetic Genomics, Inc.
    Inventors: John Verruto, Eric Moellering, Imad Ajjawi
  • Publication number: 20210238611
    Abstract: The invention provides important tools for the use of Chlorophyte organisms, including regulatory sequences useful for the expression of transgenes in such organisms. Also provided are vectors and expression cassettes containing promoters and/or terminators disclosed herein for expression of transgenes in Chlorophyte organisms. Methods of using these tools are also provided.
    Type: Application
    Filed: February 4, 2021
    Publication date: August 5, 2021
    Inventors: John Verruto, Moena Aqui
  • Publication number: 20210188924
    Abstract: Disclosed herein are mutant photosynthetic microorganisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.
    Type: Application
    Filed: February 17, 2021
    Publication date: June 24, 2021
    Inventors: Eric R. Moellering, Nicholas Bauman, Randor R. Radakovits, Roberto Spreafico, Fedor Kuzminov, Imad Ajjawi, Saheed Imam, Andrew Schultz, Kathleen Kwok, Moena Aqui, Jennifer Nominati, John Verruto, Shaun Bailey
  • Patent number: 10968259
    Abstract: Disclosed herein are mutant photosynthetic microorgnaisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.
    Type: Grant
    Filed: December 29, 2017
    Date of Patent: April 6, 2021
    Assignee: Synthetic Genomics, Inc.
    Inventors: Eric R. Moellering, Nicholas Bauman, Randor R. Radakovits, Roberto Spreafico, Fedor Kuzminov, Imad Ajjawi, Saheed Imam, Andrew Schultz, Kathleen Kwok, Moena Aqui, Jennifer Nominati, John Verruto, Shaun Bailey
  • Publication number: 20210017530
    Abstract: The present invention provides cell lines for high efficiency genome editing using cas/CRISPR systems, methods of generating such cells lines and methods of generating mutations in the genome of an organism using such cell lines.
    Type: Application
    Filed: September 30, 2020
    Publication date: January 21, 2021
    Inventors: John Verruto, Eric Moellering
  • Publication number: 20200199607
    Abstract: The present application provides novel algal regulatory elements including inducible nitrate/nitrite promoter sequences and terminator sequences. The application further discloses DNA constructs comprising these novel regulatory elements, and recombinant microorganisms comprising these regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. Methods disclosed in the present application are suited for inducible expressions of genes, such as a transgene or a native gene in algal species.
    Type: Application
    Filed: December 18, 2019
    Publication date: June 25, 2020
    Inventors: John Verruto, Jessica Greiner
  • Publication number: 20190177738
    Abstract: The present invention provides mutant microorganisms having attenuated expression of a gene encoding a polypeptide that includes a TPR domain, wherein the mutant microorganisms have higher lipid productivity and/or exhibit increased partitioning of carbon to lipid as compared to wild type microorganisms from which they are derived. Also provided are methods of producing lipids using the mutant microorganisms, guide RNAs, and nucleic acid constructs used for producing mutant microorganisms.
    Type: Application
    Filed: December 7, 2018
    Publication date: June 13, 2019
    Inventors: John Verruto, Eric Moellering, Imad Ajjawi
  • Publication number: 20180186842
    Abstract: Disclosed herein are mutant photosynthetic microorgnaisms having an attenuated SGI1 gene. The mutants have reduced chlorophyll and increased productivity with respect to wild type cells. Also disclosed are methods of using such mutants for producing biomass or bioproducts, and methods of screening for such mutants.
    Type: Application
    Filed: December 29, 2017
    Publication date: July 5, 2018
    Inventors: Eric R. Moellering, Nicholas Bauman, Randor R. Radakovits, Roberto Spreafico, Fedor Kuzminov, Imad Ajjawi, Saheed Imam, Andrew Schultz, Kathleen Kwok, Moena Aqui, Jennifer Nominati, John Verruto, Shaun Bailey
  • Publication number: 20170073695
    Abstract: The present invention provides cell lines for high efficiency genome editing using cas/CRISPR systems, methods of generating such cells lines, and methods of generating mutations in the genome of an organism using such cell lines.
    Type: Application
    Filed: December 31, 2015
    Publication date: March 16, 2017
    Inventors: John Verruto, Eric Moellering
  • Patent number: 9017975
    Abstract: The invention relates to acyl-CoA-independent methods of producing fatty alcohols in recombinant host cells engineered to express an alcohol-forming acyl-ACP reductase. The recombinant host cells may be photosynthetic microorganisms, such as cyanobacteria. Isolated nucleic acid molecules, vectors, and recombinant host cells expressing an alcohol-forming acyl-ACP reductase, and systems for producing fatty alcohols via an acyl-CoA-independent pathway, are also provided. Also provided are microorganisms engineered for the secretion of fatty acids and fatty acid derivatives, including fatty alcohols, and methods of producing fatty acid derivatives using such engineering microorganisms.
    Type: Grant
    Filed: September 27, 2012
    Date of Patent: April 28, 2015
    Assignee: Synthetic Genomics, Inc.
    Inventors: Erik Holtzapple, Robert C. Brown, Rekha Seshadri, Jennifer Coppersmith, John Verruto
  • Patent number: 8309323
    Abstract: This invention describes genes, metabolic pathways, microbial strains and methods to produce 2,6-dimethyloctane as an advanced biofuel from renewable feedstocks.
    Type: Grant
    Filed: November 13, 2008
    Date of Patent: November 13, 2012
    Assignee: Synthetic Genomics, Inc.
    Inventors: Kevin V. Martin, Stephen Picataggio, Paul Roessler, John Verruto, Kevin Watts
  • Publication number: 20110160501
    Abstract: This invention describes genes, metabolic pathways, microbial strains and methods to produce 2,6-dimethyloctane as an advanced biofuel from renewable feedstocks.
    Type: Application
    Filed: November 13, 2008
    Publication date: June 30, 2011
    Inventors: Kevin V. Martin, Stephen Picataggio, Paul Roessler, John Verruto, Kevin Watts