Patents by Inventor John W. Dubendorff

John W. Dubendorff has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cloning and expression of autogenes encoding RNA polymerases of T7-like bacteriophages
    Patent number: 5830694
    Abstract: This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods.
    Type: Grant
    Filed: November 8, 1996
    Date of Patent: November 3, 1998
    Assignee: Associated Universities, Inc.
    Inventors: F. William Studier, John W. Dubendorff
  • Cloning and expression of autogenes encoding RNA poly,erases of T7-like bacteriophages
    Patent number: 5824528
    Abstract: This invention relates to the cloning and expression of autogenes encoding RNA polymerases of T7 and T7-like bacteriophages, in which the RNA polymerase gene is transcribed from a promoter which is recognized by the encoded RNA polymerase. Cloning of T7 autogenes was achieved by reducing the activity of the RNA polymerase sufficiently to permit host cell growth. T7 RNA polymerase activity was controlled by combining two independent methods: lac-repression of the recombinant lac operator-T7 promoter in the autogene and inhibition of the polymerase by T7 lysozyme. Expression systems for producing the RNA polymerases of T7 and other T7-like bacteriophages, and expression systems for producing selected gene products are described, as well as other related materials and methods.
    Type: Grant
    Filed: August 15, 1994
    Date of Patent: October 20, 1998
    Assignee: Associated Universities, Inc.
    Inventors: F. William Studier, John W. Dubendorff