Abstract: Peptides called receptor mediated permeabilizers (RMP) increase the permeability of the blood-brain barrier to molecules such as therapeutic agents or diagnostic agents. The permeabilizer A-7 or conformational analogues can be intravenously co-administered to a host together with molecules whose desired destination is the interstitial fluid compartment of the brain. Alternatively, the permeabilizer A-7 or conformational analogues can be administered sequentially with the molecule(s) of interest and these molecules can also be administered by routes other than intravascular. The permeabilizer A-7 or conformational analogues allow these molecules to penetrate the blood-brain barrier and arrive in the interstitial fluid.

Abstract: Polypeptides called receptor mediated permeabilizers (RMP) increase the permeability of the blood-brain barrier to molecules such as therapeutic agents or diagnostic agents. These receptor mediated permeabilizers are more efficacious than bradykinin in causing the blood-brain barrier to become more permeable. The permeabilizer A-7 or conformational analogues can be intravenously co-administered to a host together with molecules whose desired destination is the cerebrospinal fluid compartment of the brain. The permeabilizer A-7 or conformational analogues allow these molecules to penetrate the blood-brain barrier and arrive at this destination.

Abstract: Polypeptides called receptor mediated permeabilizers (RMP) increase the permeability of the blood-brain barrier to molecules such as therapeutic agents or diagnostic agents. These receptor mediated permeabilizers are more efficacious than bradykinin in causing the blood-brain barrier to become more permeable. The permeabilizer A-7 or conformational analogues can be intravenously co-administered to a host together with molecules whose desired destination is the cerebrospinal fluid compartment of the brain. The permeabilizer A-7 or conformational analogues allow these molecules to penetrate the blood-brain barrier and arrive at this destination.

Abstract: The synthesis of a new affinity medium, the p-aminophenyl-.gamma.-ester of 7-methylguanosine-5'-triphosphate coupled to Sepharose, for use in affinity chromatography is claimed. An improved method in terms of speed and purity for the isolation of messenger ribonucleic acid cap-binding protein is claimed. The synthesis of the p-aminophenyl-.gamma.-ester of 7-methylguanosine-5'-triphosphate is described.

Abstract: Alkylation processes wherein diazoalkane alkylation reagents are generated in situ and utilized to alkylate substrates are disclosed. These processes utilize the relatively slow base-catalyzed decomposition of an N-alkyl, N-nitrosourea to produce a corresponding diazoalkane alkylation reagent, followed by the much faster reaction between the alkylation reagent and the substrate to be alkylated. Generically, suitable ureas can be represented by the structural formula, ##EQU1## wherein R represents the alkyl moiety to be added to the substrate; R can be alkyl, cycloalkyl, heteroalkyl, aralkyl or heterocyclic.This process is useful in producing alkylated products without the concomitant disadvantage of building up significant amounts of diazoalkane alkylation reagents during the course of the reaction, many of which have deleterious properties.